Development and Validation of HPLC method for simultaneous estimation of Rifampicin and Ofloxacin using experimental design
A accurate, precise and robust isocratic HPLC method has been developed and validated for simultaneous determination of Rifampicin and Ofloxacin. The chromatographic separation was carried out on Kinetex C18, 100 A Phenomenex column with a mixture of 0.03M Potassium dihydrogen phosphate buffer pH 3....
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Journal of Taibah University for Science |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/16583655.2018.1548748 |
| _version_ | 1818148108314345472 |
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| author | Purvi Shah Tosha Pandya Mukesh Gohel Vaishali Thakkar |
| author_facet | Purvi Shah Tosha Pandya Mukesh Gohel Vaishali Thakkar |
| author_sort | Purvi Shah |
| collection | DOAJ |
| description | A accurate, precise and robust isocratic HPLC method has been developed and validated for simultaneous determination of Rifampicin and Ofloxacin. The chromatographic separation was carried out on Kinetex C18, 100 A Phenomenex column with a mixture of 0.03M Potassium dihydrogen phosphate buffer pH 3.0: Acetonitrile (55:45) as mobile phase at 230 nm. The retention times were 2.91 and 4.87 min for Ofloxacin and Rifampicin, respectively. Calibration plots were linear over the concentration range 1–5 and 2–10 µg/ml for Rifampicin and Ofloxacin, respectively. The method was validated for linearity, sensitivity accuracy, precision, and robustness. Percent recoveries were found to be close to 100% with low variability. Fractional factorial design with four factors was chosen for robustness testing. The volume of acetonitrile and flow rate showed significant effect on retention factor of both the drugs and asymmetry factor of ofloxacin. The method may be adopted for routine analysis at industry. |
| first_indexed | 2024-12-11T12:45:54Z |
| format | Article |
| id | doaj.art-d1873d844e2648478304a2eaabcaf186 |
| institution | Directory Open Access Journal |
| issn | 1658-3655 |
| language | English |
| last_indexed | 2024-12-11T12:45:54Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Taibah University for Science |
| spelling | doaj.art-d1873d844e2648478304a2eaabcaf1862022-12-22T01:06:50ZengTaylor & Francis GroupJournal of Taibah University for Science1658-36552019-12-0113114615410.1080/16583655.2018.15487481548748Development and Validation of HPLC method for simultaneous estimation of Rifampicin and Ofloxacin using experimental designPurvi Shah0Tosha Pandya1Mukesh Gohel2Vaishali Thakkar3Anand Pharmacy CollegeAnand Pharmacy CollegeAnand Pharmacy CollegeAnand Pharmacy CollegeA accurate, precise and robust isocratic HPLC method has been developed and validated for simultaneous determination of Rifampicin and Ofloxacin. The chromatographic separation was carried out on Kinetex C18, 100 A Phenomenex column with a mixture of 0.03M Potassium dihydrogen phosphate buffer pH 3.0: Acetonitrile (55:45) as mobile phase at 230 nm. The retention times were 2.91 and 4.87 min for Ofloxacin and Rifampicin, respectively. Calibration plots were linear over the concentration range 1–5 and 2–10 µg/ml for Rifampicin and Ofloxacin, respectively. The method was validated for linearity, sensitivity accuracy, precision, and robustness. Percent recoveries were found to be close to 100% with low variability. Fractional factorial design with four factors was chosen for robustness testing. The volume of acetonitrile and flow rate showed significant effect on retention factor of both the drugs and asymmetry factor of ofloxacin. The method may be adopted for routine analysis at industry.http://dx.doi.org/10.1080/16583655.2018.1548748rifampicinofloxacinfractional factorial designhigh performance liquid chromatographyvalidation |
| spellingShingle | Purvi Shah Tosha Pandya Mukesh Gohel Vaishali Thakkar Development and Validation of HPLC method for simultaneous estimation of Rifampicin and Ofloxacin using experimental design Journal of Taibah University for Science rifampicin ofloxacin fractional factorial design high performance liquid chromatography validation |
| title | Development and Validation of HPLC method for simultaneous estimation of Rifampicin and Ofloxacin using experimental design |
| title_full | Development and Validation of HPLC method for simultaneous estimation of Rifampicin and Ofloxacin using experimental design |
| title_fullStr | Development and Validation of HPLC method for simultaneous estimation of Rifampicin and Ofloxacin using experimental design |
| title_full_unstemmed | Development and Validation of HPLC method for simultaneous estimation of Rifampicin and Ofloxacin using experimental design |
| title_short | Development and Validation of HPLC method for simultaneous estimation of Rifampicin and Ofloxacin using experimental design |
| title_sort | development and validation of hplc method for simultaneous estimation of rifampicin and ofloxacin using experimental design |
| topic | rifampicin ofloxacin fractional factorial design high performance liquid chromatography validation |
| url | http://dx.doi.org/10.1080/16583655.2018.1548748 |
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