Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz)
KT/HAK/KUP (KUP) family is responsible for potassium ion (K+) transport, which plays a vital role in the response of plants to abiotic stress by maintaining osmotic balance. However, our understanding of the functions of the KUP family in the drought-resistant crop cassava (Manihot esculenta Crantz)...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-01-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2018.00017/full |
| _version_ | 1819067654496845824 |
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| author | Wenjun Ou Xiang Mao Chao Huang Weiwei Tie Yan Yan Zehong Ding Chunlai Wu Zhiqiang Xia Wenquan Wang Shiyi Zhou Kaimian Li Kaimian Li Wei Hu |
| author_facet | Wenjun Ou Xiang Mao Chao Huang Weiwei Tie Yan Yan Zehong Ding Chunlai Wu Zhiqiang Xia Wenquan Wang Shiyi Zhou Kaimian Li Kaimian Li Wei Hu |
| author_sort | Wenjun Ou |
| collection | DOAJ |
| description | KT/HAK/KUP (KUP) family is responsible for potassium ion (K+) transport, which plays a vital role in the response of plants to abiotic stress by maintaining osmotic balance. However, our understanding of the functions of the KUP family in the drought-resistant crop cassava (Manihot esculenta Crantz) is limited. In the present study, 21 cassava KUP genes (MeKUPs) were identified and classified into four clusters based on phylogenetic relationships, conserved motifs, and gene structure analyses. Transcriptome analysis revealed the expression diversity of cassava KUPs in various tissues of three genotypes. Comparative transcriptome analysis showed that the activation of MeKUP genes by drought was more in roots than that in leaves of Arg7 and W14 genotypes, whereas less in roots than that in leaves of SC124 variety. These findings indicate that different cassava genotypes utilize various drought resistance mechanism mediated by KUP genes. Specific KUP genes showed broad upregulation after exposure to salt, osmotic, cold, H2O2, and abscisic acid (ABA) treatments. Taken together, this study provides insights into the KUP-mediated drought response of cassava at transcription levels and identifies candidate genes that may be utilized in improving crop tolerance to abiotic stress. |
| first_indexed | 2024-12-21T16:21:41Z |
| format | Article |
| id | doaj.art-d187e8ee889c445ab5c262e13db1ef9e |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-21T16:21:41Z |
| publishDate | 2018-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-d187e8ee889c445ab5c262e13db1ef9e2022-12-21T18:57:34ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2018-01-01910.3389/fphys.2018.00017287897Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz)Wenjun Ou0Xiang Mao1Chao Huang2Weiwei Tie3Yan Yan4Zehong Ding5Chunlai Wu6Zhiqiang Xia7Wenquan Wang8Shiyi Zhou9Kaimian Li10Kaimian Li11Wei Hu12Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, ChinaWuhan Centre for Disease Prevention and Control, Wuhan, ChinaCollege of Life Science and Technology, Huazhong University of Science and Technology (HUST), Wuhan, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaHubei Key Laboratory of Purification and Application of Plant Anticancer Active Ingredients, Chemistry and Biology Science College, Hubei University of Education, Wuhan, ChinaTropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKey Laboratory of Biology and Genetic Resources of Tropical Crops, Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou, ChinaKT/HAK/KUP (KUP) family is responsible for potassium ion (K+) transport, which plays a vital role in the response of plants to abiotic stress by maintaining osmotic balance. However, our understanding of the functions of the KUP family in the drought-resistant crop cassava (Manihot esculenta Crantz) is limited. In the present study, 21 cassava KUP genes (MeKUPs) were identified and classified into four clusters based on phylogenetic relationships, conserved motifs, and gene structure analyses. Transcriptome analysis revealed the expression diversity of cassava KUPs in various tissues of three genotypes. Comparative transcriptome analysis showed that the activation of MeKUP genes by drought was more in roots than that in leaves of Arg7 and W14 genotypes, whereas less in roots than that in leaves of SC124 variety. These findings indicate that different cassava genotypes utilize various drought resistance mechanism mediated by KUP genes. Specific KUP genes showed broad upregulation after exposure to salt, osmotic, cold, H2O2, and abscisic acid (ABA) treatments. Taken together, this study provides insights into the KUP-mediated drought response of cassava at transcription levels and identifies candidate genes that may be utilized in improving crop tolerance to abiotic stress.http://journal.frontiersin.org/article/10.3389/fphys.2018.00017/fullcassavadrought stressgene expressionidentificationKUP family |
| spellingShingle | Wenjun Ou Xiang Mao Chao Huang Weiwei Tie Yan Yan Zehong Ding Chunlai Wu Zhiqiang Xia Wenquan Wang Shiyi Zhou Kaimian Li Kaimian Li Wei Hu Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz) Frontiers in Physiology cassava drought stress gene expression identification KUP family |
| title | Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz) |
| title_full | Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz) |
| title_fullStr | Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz) |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz) |
| title_short | Genome-Wide Identification and Expression Analysis of the KUP Family under Abiotic Stress in Cassava (Manihot esculenta Crantz) |
| title_sort | genome wide identification and expression analysis of the kup family under abiotic stress in cassava manihot esculenta crantz |
| topic | cassava drought stress gene expression identification KUP family |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2018.00017/full |
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