Influence of different methods and anticoagulants on platelet parameter measurement
Platelets are the smallest and perhaps the most versatile components of human blood. Besides their role in coagulation and the maintenance of vascular integrity, they are involved in many physiological processes, ranging from immune response and leukocyte recruitment to the production of antimicrobi...
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2020-10-01
|
| Series: | Journal of Laboratory Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/labmed-2020-0037 |
| _version_ | 1819063651871490048 |
|---|---|
| author | Mannuß Steffen |
| author_facet | Mannuß Steffen |
| author_sort | Mannuß Steffen |
| collection | DOAJ |
| description | Platelets are the smallest and perhaps the most versatile components of human blood. Besides their role in coagulation and the maintenance of vascular integrity, they are involved in many physiological processes, ranging from immune response and leukocyte recruitment to the production of antimicrobial peptides and immune-suppressive factors like TGF-β. These versatile abilities make platelets interesting for researchers from different disciplines. However, beside profound investigation into platelets’ physiological role, there is a need for correct, standardized and thus reproducible quantification of platelet parameters. Mean platelet volume (MPV) is a widespread prognostic marker for several conditions, such as, acute coronary syndrome, chronic kidney disease and liver cirrhosis. Platelet activation is regarded as a marker for inflammatory processes, for example in autoimmune diseases such as type-1 diabetes, systemic lupus erythematosus and rheumatoid arthritis. The monitoring of platelet function is relevant for patients receiving antiplatelet medication. Platelet parameter measurement is affected by the choice of in vitro anticoagulant, the measurement technology and the time delay after sampling. This review focuses on the pre-analytical variability that arises as a result of the use of different in vitro anticoagulants and analyzer technologies when determining platelet parameters, since, even approximately 180 years after the discovery of platelets, there is still no standardized procedure. |
| first_indexed | 2024-12-21T15:18:04Z |
| format | Article |
| id | doaj.art-d188b7b31a0844bb8b0c934e06005564 |
| institution | Directory Open Access Journal |
| issn | 2567-9430 2567-9449 |
| language | English |
| last_indexed | 2024-12-21T15:18:04Z |
| publishDate | 2020-10-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Journal of Laboratory Medicine |
| spelling | doaj.art-d188b7b31a0844bb8b0c934e060055642022-12-21T18:59:06ZengDe GruyterJournal of Laboratory Medicine2567-94302567-94492020-10-0144525527210.1515/labmed-2020-0037Influence of different methods and anticoagulants on platelet parameter measurementMannuß Steffen0Klinikum der Stadt Ludwigshafen, Institut für Labordiagnostik, Hygiene und Transfusionsmedizin, Ludwigshafen, GermanyPlatelets are the smallest and perhaps the most versatile components of human blood. Besides their role in coagulation and the maintenance of vascular integrity, they are involved in many physiological processes, ranging from immune response and leukocyte recruitment to the production of antimicrobial peptides and immune-suppressive factors like TGF-β. These versatile abilities make platelets interesting for researchers from different disciplines. However, beside profound investigation into platelets’ physiological role, there is a need for correct, standardized and thus reproducible quantification of platelet parameters. Mean platelet volume (MPV) is a widespread prognostic marker for several conditions, such as, acute coronary syndrome, chronic kidney disease and liver cirrhosis. Platelet activation is regarded as a marker for inflammatory processes, for example in autoimmune diseases such as type-1 diabetes, systemic lupus erythematosus and rheumatoid arthritis. The monitoring of platelet function is relevant for patients receiving antiplatelet medication. Platelet parameter measurement is affected by the choice of in vitro anticoagulant, the measurement technology and the time delay after sampling. This review focuses on the pre-analytical variability that arises as a result of the use of different in vitro anticoagulants and analyzer technologies when determining platelet parameters, since, even approximately 180 years after the discovery of platelets, there is still no standardized procedure.https://doi.org/10.1515/labmed-2020-0037in vitro anticoagulationmean platelet volumemeasurement technologyplatelet activationplatelet countingplatelet function |
| spellingShingle | Mannuß Steffen Influence of different methods and anticoagulants on platelet parameter measurement Journal of Laboratory Medicine in vitro anticoagulation mean platelet volume measurement technology platelet activation platelet counting platelet function |
| title | Influence of different methods and anticoagulants on platelet parameter measurement |
| title_full | Influence of different methods and anticoagulants on platelet parameter measurement |
| title_fullStr | Influence of different methods and anticoagulants on platelet parameter measurement |
| title_full_unstemmed | Influence of different methods and anticoagulants on platelet parameter measurement |
| title_short | Influence of different methods and anticoagulants on platelet parameter measurement |
| title_sort | influence of different methods and anticoagulants on platelet parameter measurement |
| topic | in vitro anticoagulation mean platelet volume measurement technology platelet activation platelet counting platelet function |
| url | https://doi.org/10.1515/labmed-2020-0037 |
| work_keys_str_mv | AT mannußsteffen influenceofdifferentmethodsandanticoagulantsonplateletparametermeasurement |