Catecholamine stress hormones promote the cell cycle re-entry of G0 phase in mouse hepatocarcinoma cell line H22
Objective To investigate the effect of stress hormones on cell cycle re-entry of G0 phase in mouse H22 hepatocarcinoma cells. Methods Flow cytometry antibody staining with CD44 and CD133 was used to determine the presence of cancer stem cells in H22 cells. In vitro, after treatment of H22 cells w...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2020-07-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2020-40-7-897.pdf |
| _version_ | 1827390481434148864 |
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| author | CAO Ming-yue, LI Gao-xiang, CHEN Yu-zhu, HUANG Wei, YANG Nan, LIU Yan-yong |
| author_facet | CAO Ming-yue, LI Gao-xiang, CHEN Yu-zhu, HUANG Wei, YANG Nan, LIU Yan-yong |
| author_sort | CAO Ming-yue, LI Gao-xiang, CHEN Yu-zhu, HUANG Wei, YANG Nan, LIU Yan-yong |
| collection | DOAJ |
| description | Objective To investigate the effect of stress hormones on cell cycle re-entry of G0 phase in mouse H22 hepatocarcinoma cells. Methods Flow cytometry antibody staining with CD44 and CD133 was used to determine the presence of cancer stem cells in H22 cells. In vitro, after treatment of H22 cells with stress hormone corticosterone (CORT), epinephrine (EPI) and norepinephrine (NE) for 48 h, the proportion changes of cells in G0 phase and cell cycle distribution were detected by Ki-67 and PI flow cytometry staining. The hormone receptor antagonists were used to verify the receptor subtypes that mediated the cell cycle re-entry of G0 phase cells. Results The proportion of cancer stem cells (CD44+CD133+) in H22 cells was 0.80%±0.15%. Compared with control group, the portion of G0 phase cells was unchanged after corticosterone treatment, but was decreased by 62.50% and 53.00% respectively after EPI and NE treatment. Meanwhile, the total cells, proportion of G2/M and S phase cells were increased compared to control group. The protein expression of Skp2 was increased and p27kip1 decreased significantly after EPI and NE treatment(P＜0.05). In addition, α1-adrenergic receptor antagonists terazosin and prazosin blocked the G0 phase cells cycle re-entry effect of EPI and NE. The protein expression of Skp2 was decreased and p27kip1 was increased significantly after prazosin treatment compared to EPI or NE group(P＜0.05). Conclusions Catecholamine stress hormones mediate the cell cycle re-entry of G0 phase cells in mouse H22 hepatocarcinoma cells by activating α1-adrenergic receptor,the mechanism of which may be associated with the Skp2 over-expression and the degradation of p27kip1 protein. |
| first_indexed | 2024-03-08T16:53:20Z |
| format | Article |
| id | doaj.art-d18a2401840f43dc9bdd23bcc9fac7bf |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T16:53:20Z |
| publishDate | 2020-07-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-d18a2401840f43dc9bdd23bcc9fac7bf2024-01-05T03:16:48ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252020-07-01407897902Catecholamine stress hormones promote the cell cycle re-entry of G0 phase in mouse hepatocarcinoma cell line H22CAO Ming-yue, LI Gao-xiang, CHEN Yu-zhu, HUANG Wei, YANG Nan, LIU Yan-yong01. Department of Pharmacology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005; ;2. Medical College, Tibet University, Lasa 850000, ChinaObjective To investigate the effect of stress hormones on cell cycle re-entry of G0 phase in mouse H22 hepatocarcinoma cells. Methods Flow cytometry antibody staining with CD44 and CD133 was used to determine the presence of cancer stem cells in H22 cells. In vitro, after treatment of H22 cells with stress hormone corticosterone (CORT), epinephrine (EPI) and norepinephrine (NE) for 48 h, the proportion changes of cells in G0 phase and cell cycle distribution were detected by Ki-67 and PI flow cytometry staining. The hormone receptor antagonists were used to verify the receptor subtypes that mediated the cell cycle re-entry of G0 phase cells. Results The proportion of cancer stem cells (CD44+CD133+) in H22 cells was 0.80%±0.15%. Compared with control group, the portion of G0 phase cells was unchanged after corticosterone treatment, but was decreased by 62.50% and 53.00% respectively after EPI and NE treatment. Meanwhile, the total cells, proportion of G2/M and S phase cells were increased compared to control group. The protein expression of Skp2 was increased and p27kip1 decreased significantly after EPI and NE treatment(P＜0.05). In addition, α1-adrenergic receptor antagonists terazosin and prazosin blocked the G0 phase cells cycle re-entry effect of EPI and NE. The protein expression of Skp2 was decreased and p27kip1 was increased significantly after prazosin treatment compared to EPI or NE group(P＜0.05). Conclusions Catecholamine stress hormones mediate the cell cycle re-entry of G0 phase cells in mouse H22 hepatocarcinoma cells by activating α1-adrenergic receptor,the mechanism of which may be associated with the Skp2 over-expression and the degradation of p27kip1 protein.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2020-40-7-897.pdfhepatocellular carcinoma|g0 phase cells|stress hormones|catecholamine|adrenergic receptor |
| spellingShingle | CAO Ming-yue, LI Gao-xiang, CHEN Yu-zhu, HUANG Wei, YANG Nan, LIU Yan-yong Catecholamine stress hormones promote the cell cycle re-entry of G0 phase in mouse hepatocarcinoma cell line H22 Jichu yixue yu linchuang hepatocellular carcinoma|g0 phase cells|stress hormones|catecholamine|adrenergic receptor |
| title | Catecholamine stress hormones promote the cell cycle re-entry of G0 phase in mouse hepatocarcinoma cell line H22 |
| title_full | Catecholamine stress hormones promote the cell cycle re-entry of G0 phase in mouse hepatocarcinoma cell line H22 |
| title_fullStr | Catecholamine stress hormones promote the cell cycle re-entry of G0 phase in mouse hepatocarcinoma cell line H22 |
| title_full_unstemmed | Catecholamine stress hormones promote the cell cycle re-entry of G0 phase in mouse hepatocarcinoma cell line H22 |
| title_short | Catecholamine stress hormones promote the cell cycle re-entry of G0 phase in mouse hepatocarcinoma cell line H22 |
| title_sort | catecholamine stress hormones promote the cell cycle re entry of g0 phase in mouse hepatocarcinoma cell line h22 |
| topic | hepatocellular carcinoma|g0 phase cells|stress hormones|catecholamine|adrenergic receptor |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2020-40-7-897.pdf |
| work_keys_str_mv | AT caomingyueligaoxiangchenyuzhuhuangweiyangnanliuyanyong catecholaminestresshormonespromotethecellcyclereentryofg0phaseinmousehepatocarcinomacelllineh22 |