Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposure
Mn (Manganese, Mn) is an essential trace element involved in various biological processes such as the regulation of immune, nervous and digestive system functions. However, excessive Mn exposure can lead to immune damage. Occupational workers in cement and ferroalloy manufacturing and other related...
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Frontiers Media S.A.
2023-11-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2023.1289838/full |
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| author | Yuanmeng Qi Huifang Si Huifang Si Xiaofei Jin Yonghua Guo Jiarui Xia Jing He Xuedan Deng Meng Deng Wu Yao Changfu Hao |
| author_facet | Yuanmeng Qi Huifang Si Huifang Si Xiaofei Jin Yonghua Guo Jiarui Xia Jing He Xuedan Deng Meng Deng Wu Yao Changfu Hao |
| author_sort | Yuanmeng Qi |
| collection | DOAJ |
| description | Mn (Manganese, Mn) is an essential trace element involved in various biological processes such as the regulation of immune, nervous and digestive system functions. However, excessive Mn exposure can lead to immune damage. Occupational workers in cement and ferroalloy manufacturing and other related industries are exposed to low levels of Mn for a long time. Mn exposure is one of the important occupational hazards, but the research on the effect of Mn on the immune system of the occupational population is not complete, and there is no reliable biomarker. Therefore, this study aimed to evaluate the immunotoxicity of Mn from the soluble immune checkpoint TIM-3 (T-cell immunoglobulin and mucin containing protein 3, TIM-3) and complement C3. A total of 144 Mn-exposed workers were recruited from a bus manufacturing company and a railroad company in Henan Province. An inductively coupled plasma mass spectrometer was used to detect the concentration of RBC Mn (Red blood cell Mn, RBC Mn), and ELISA kits were used to detect serum complement C3 and TIM-3. Finally, the subjects were statistically analyzed by dividing them into low and high Mn groups based on the median RBC Mn concentration. We found that Mn exposure resulted in elevated serum TIM-3 expression and decreased complement C3 expression in workers; that serum TIM-3 and complement C3 expression showed a dose–response relationship with RBC Mn; and that the mediating effect of complement C3 between RBC Mn and TIM-3 was found to be significant. The above findings indicate that this study has a preliminary understanding of the effect of Mn exposure on the immune system of the occupational population exposed to Mn, and complement C3 and TIM-3 may be biomarkers of Mn exposure, which may provide clues for the prevention and control of Mn occupational hazards. |
| first_indexed | 2024-03-11T10:57:38Z |
| format | Article |
| id | doaj.art-d18a40a4968c4c038459d3ab36d9bbf1 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-03-11T10:57:38Z |
| publishDate | 2023-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj.art-d18a40a4968c4c038459d3ab36d9bbf12023-11-13T04:40:48ZengFrontiers Media S.A.Frontiers in Public Health2296-25652023-11-011110.3389/fpubh.2023.12898381289838Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposureYuanmeng Qi0Huifang Si1Huifang Si2Xiaofei Jin3Yonghua Guo4Jiarui Xia5Jing He6Xuedan Deng7Meng Deng8Wu Yao9Changfu Hao10Department of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaPrevention and Infection Control Section, Xi’an Union Hospital, Xi’an, Shanxi, ChinaDepartment of Ultrasound, The Third Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Occupational and Environment Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Child and Adolescence Health, College of Public Health, Zhengzhou University, Zhengzhou, Henan, ChinaMn (Manganese, Mn) is an essential trace element involved in various biological processes such as the regulation of immune, nervous and digestive system functions. However, excessive Mn exposure can lead to immune damage. Occupational workers in cement and ferroalloy manufacturing and other related industries are exposed to low levels of Mn for a long time. Mn exposure is one of the important occupational hazards, but the research on the effect of Mn on the immune system of the occupational population is not complete, and there is no reliable biomarker. Therefore, this study aimed to evaluate the immunotoxicity of Mn from the soluble immune checkpoint TIM-3 (T-cell immunoglobulin and mucin containing protein 3, TIM-3) and complement C3. A total of 144 Mn-exposed workers were recruited from a bus manufacturing company and a railroad company in Henan Province. An inductively coupled plasma mass spectrometer was used to detect the concentration of RBC Mn (Red blood cell Mn, RBC Mn), and ELISA kits were used to detect serum complement C3 and TIM-3. Finally, the subjects were statistically analyzed by dividing them into low and high Mn groups based on the median RBC Mn concentration. We found that Mn exposure resulted in elevated serum TIM-3 expression and decreased complement C3 expression in workers; that serum TIM-3 and complement C3 expression showed a dose–response relationship with RBC Mn; and that the mediating effect of complement C3 between RBC Mn and TIM-3 was found to be significant. The above findings indicate that this study has a preliminary understanding of the effect of Mn exposure on the immune system of the occupational population exposed to Mn, and complement C3 and TIM-3 may be biomarkers of Mn exposure, which may provide clues for the prevention and control of Mn occupational hazards.https://www.frontiersin.org/articles/10.3389/fpubh.2023.1289838/fullMn-exposed workersimmunotoxicityRBC MnTIM-3complement C3 |
| spellingShingle | Yuanmeng Qi Huifang Si Huifang Si Xiaofei Jin Yonghua Guo Jiarui Xia Jing He Xuedan Deng Meng Deng Wu Yao Changfu Hao Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposure Frontiers in Public Health Mn-exposed workers immunotoxicity RBC Mn TIM-3 complement C3 |
| title | Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposure |
| title_full | Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposure |
| title_fullStr | Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposure |
| title_full_unstemmed | Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposure |
| title_short | Changes in serum TIM-3 and complement C3 expression in workers due to Mn exposure |
| title_sort | changes in serum tim 3 and complement c3 expression in workers due to mn exposure |
| topic | Mn-exposed workers immunotoxicity RBC Mn TIM-3 complement C3 |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2023.1289838/full |
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