Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives
Powdery mildew is one of the most severe diseases affecting wheat yield and quality and is caused by Blumeria graminis f. sp. tritici (Bgt). Host resistance is the preferred strategy to prevent this disease. However, the narrow genetic basis of common wheat has increased the demand for diversified g...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2024-01-01
|
| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2024.1342239/full |
| _version_ | 1797347913174614016 |
|---|---|
| author | Jiaojiao Wang Hongxing Xu Yanmin Qie Ran Han Xiaohui Sun Ya Zhao Bei Xiao Zejun Qian Xiaomei Huang Ruishan Liu Jiadong Zhang Cheng Liu Yuli Jin Pengtao Ma |
| author_facet | Jiaojiao Wang Hongxing Xu Yanmin Qie Ran Han Xiaohui Sun Ya Zhao Bei Xiao Zejun Qian Xiaomei Huang Ruishan Liu Jiadong Zhang Cheng Liu Yuli Jin Pengtao Ma |
| author_sort | Jiaojiao Wang |
| collection | DOAJ |
| description | Powdery mildew is one of the most severe diseases affecting wheat yield and quality and is caused by Blumeria graminis f. sp. tritici (Bgt). Host resistance is the preferred strategy to prevent this disease. However, the narrow genetic basis of common wheat has increased the demand for diversified germplasm resources against powdery mildew. Wheat relatives, especially the secondary gene pool of common wheat, are important gene donors in the genetic improvement of common wheat because of its abundant genetic variation and close kinship with wheat. In this study, a series of 137 wheat relatives, including 53 Triticum monococcum L. (2n = 2x = 14, AA), 6 T. urartu Thumanjan ex Gandilyan (2n = 2x = 14, AA), 9 T. timopheevii Zhuk. (2n = 4x = 28, AAGG), 66 T. aestivum subsp. spelta (2n = 6x = 42, AABBDD), and 3 Aegilops speltoides (2n = 2x = 14, SS) were systematically evaluated for their powdery mildew resistance and composition of Pm genes. Out of 137 (60.58%) accessions, 83 were resistant to Bgt isolate E09 at the seedling stage, and 116 of 137 (84.67%) wheat relatives were resistant to the mixture of Bgt isolates at the adult stage. This indicates that these accessions show a high level of resistance to powdery mildew. Some 31 markers for 23 known Pm genes were used to test these 137 accessions, and, in the results, only Pm2, Pm4, Pm6, Pm58, and Pm68 were detected. Among them, three Pm4 alleles (Pm4a, Pm4b, and Pm4f) were identified in 4 T. subsp. spelta accessions. q-RT PCR further confirmed that Pm4 alleles played a role in disease resistance in these four accessions. The phylogenetic tree showed that the kinship of Pm4 was close to Pm24 and Sr62. This study not only provides reference information and valuable germplasm resources for breeding new wheat varieties with disease resistance but also lays a foundation for enriching the genetic basis of wheat resistance to powdery mildew. |
| first_indexed | 2024-03-08T11:54:48Z |
| format | Article |
| id | doaj.art-d18c204c323a4f74b51779455761e740 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-03-08T11:54:48Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-d18c204c323a4f74b51779455761e7402024-01-24T04:47:14ZengFrontiers Media S.A.Frontiers in Genetics1664-80212024-01-011510.3389/fgene.2024.13422391342239Evaluation and identification of powdery mildew-resistant genes in 137 wheat relativesJiaojiao Wang0Hongxing Xu1Yanmin Qie2Ran Han3Xiaohui Sun4Ya Zhao5Bei Xiao6Zejun Qian7Xiaomei Huang8Ruishan Liu9Jiadong Zhang10Cheng Liu11Yuli Jin12Pengtao Ma13Yantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaSchool of Life Sciences, Henan University, Kaifeng, Henan, ChinaInstitute of Cereal and Oil Crops, Hebei Academy of Agricultural and Forestry Sciences, Hebei Key Laboratory of Crop Genetics and Breeding, Shijiazhuang, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, ChinaInstitute of Grain and Oil Crops, Yantai Academy of Agricultural Science, Yantai, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaCrop Research Institute, Shandong Academy of Agricultural Sciences, Jinan, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaYantai Key Laboratory of Characteristic Agricultural Bioresource Conservation & Germplasm Innovative Utilization, College of Life Sciences, Yantai University, Yantai, ChinaPowdery mildew is one of the most severe diseases affecting wheat yield and quality and is caused by Blumeria graminis f. sp. tritici (Bgt). Host resistance is the preferred strategy to prevent this disease. However, the narrow genetic basis of common wheat has increased the demand for diversified germplasm resources against powdery mildew. Wheat relatives, especially the secondary gene pool of common wheat, are important gene donors in the genetic improvement of common wheat because of its abundant genetic variation and close kinship with wheat. In this study, a series of 137 wheat relatives, including 53 Triticum monococcum L. (2n = 2x = 14, AA), 6 T. urartu Thumanjan ex Gandilyan (2n = 2x = 14, AA), 9 T. timopheevii Zhuk. (2n = 4x = 28, AAGG), 66 T. aestivum subsp. spelta (2n = 6x = 42, AABBDD), and 3 Aegilops speltoides (2n = 2x = 14, SS) were systematically evaluated for their powdery mildew resistance and composition of Pm genes. Out of 137 (60.58%) accessions, 83 were resistant to Bgt isolate E09 at the seedling stage, and 116 of 137 (84.67%) wheat relatives were resistant to the mixture of Bgt isolates at the adult stage. This indicates that these accessions show a high level of resistance to powdery mildew. Some 31 markers for 23 known Pm genes were used to test these 137 accessions, and, in the results, only Pm2, Pm4, Pm6, Pm58, and Pm68 were detected. Among them, three Pm4 alleles (Pm4a, Pm4b, and Pm4f) were identified in 4 T. subsp. spelta accessions. q-RT PCR further confirmed that Pm4 alleles played a role in disease resistance in these four accessions. The phylogenetic tree showed that the kinship of Pm4 was close to Pm24 and Sr62. This study not only provides reference information and valuable germplasm resources for breeding new wheat varieties with disease resistance but also lays a foundation for enriching the genetic basis of wheat resistance to powdery mildew.https://www.frontiersin.org/articles/10.3389/fgene.2024.1342239/fullpowdery mildewmarkersresistance identificationhaplotype analysiswheat relatives |
| spellingShingle | Jiaojiao Wang Hongxing Xu Yanmin Qie Ran Han Xiaohui Sun Ya Zhao Bei Xiao Zejun Qian Xiaomei Huang Ruishan Liu Jiadong Zhang Cheng Liu Yuli Jin Pengtao Ma Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives Frontiers in Genetics powdery mildew markers resistance identification haplotype analysis wheat relatives |
| title | Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives |
| title_full | Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives |
| title_fullStr | Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives |
| title_full_unstemmed | Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives |
| title_short | Evaluation and identification of powdery mildew-resistant genes in 137 wheat relatives |
| title_sort | evaluation and identification of powdery mildew resistant genes in 137 wheat relatives |
| topic | powdery mildew markers resistance identification haplotype analysis wheat relatives |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2024.1342239/full |
| work_keys_str_mv | AT jiaojiaowang evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT hongxingxu evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT yanminqie evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT ranhan evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT xiaohuisun evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT yazhao evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT beixiao evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT zejunqian evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT xiaomeihuang evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT ruishanliu evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT jiadongzhang evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT chengliu evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT yulijin evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives AT pengtaoma evaluationandidentificationofpowderymildewresistantgenesin137wheatrelatives |