Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus
Background and Aim: Antimicrobial resistance is a global problem caused by extensive utilization of antibiotics that promote gene resistant among bacteria, including Staphylococcus aureus. This study aimed to analyze the potential effects of celery (Apium graveolens) extract as an antioxidant and an...
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| Format: | Article |
| Language: | English |
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Veterinary World
2020-05-01
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| Series: | Veterinary World |
| Subjects: | |
| Online Access: | http://www.veterinaryworld.org/Vol.13/May-2020/5.pdf |
| _version_ | 1819114496396886016 |
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| author | Yos Adi Prakoso Chylen Setiyo Rini Asih Rahayu Miarsono Sigit Dyah Widhowati |
| author_facet | Yos Adi Prakoso Chylen Setiyo Rini Asih Rahayu Miarsono Sigit Dyah Widhowati |
| author_sort | Yos Adi Prakoso |
| collection | DOAJ |
| description | Background and Aim: Antimicrobial resistance is a global problem caused by extensive utilization of antibiotics that promote gene resistant among bacteria, including Staphylococcus aureus. This study aimed to analyze the potential effects of celery (Apium graveolens) extract as an antioxidant and antimicrobial agent against methicillin-resistant S. aureus (MRSA), in vitro and in vivo.
Materials and Methods: Celery was extracted and tested against a MRSA isolate in vitro. The minimum inhibitory concentration (MIC) against MRSA for the celery extract (CE) was determined to be 0.1% and it was formulated into a cream. A total of 30 female Sprague Dawley rats were divided into five groups: Group 1, negative control; Group 2, positive control; Group 3, treated with 0.05% CE cream; Group 4, 0.1% CE cream; and Group 5, 0.2% CE cream. All animals in the groups were exposed to a full-thickness skin biopsy on the dorsal portion, and they were infected with 30 μL of 105 colony-forming units of the MRSA isolate. The treatment was administered twice a day for 7 days. The skin samples were collected on days 3 and 7 after the treatment. The skin tissue was examined histologically using hematoxylin and eosin, Gram staining, and immunohistochemistry against cytokeratin (CK)-17.
Results: Results showed that 0.2% of CE cream was the best treatment for wounds infected with MRSA. CE (0.2%) cream increased skin reepithelialization, fibroblast proliferation, and CK-17 expression; it also decreased the percentage of wound area, inflammatory cell infiltration, and bacterial colonization in skin wound tissue compared to the other treatments (p≤0.05).
Conclusion: This study demonstrated that celery could be utilized as an alternative herbal therapy against MRSA-associated skin infections. |
| first_indexed | 2024-12-22T04:46:13Z |
| format | Article |
| id | doaj.art-d192373151a946c49699546ee4d7dcbe |
| institution | Directory Open Access Journal |
| issn | 0972-8988 2231-0916 |
| language | English |
| last_indexed | 2024-12-22T04:46:13Z |
| publishDate | 2020-05-01 |
| publisher | Veterinary World |
| record_format | Article |
| series | Veterinary World |
| spelling | doaj.art-d192373151a946c49699546ee4d7dcbe2022-12-21T18:38:36ZengVeterinary WorldVeterinary World0972-89882231-09162020-05-0113586587110.14202/vetworld.2020.865-871Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureusYos Adi Prakoso0https://orcid.org/0000-0003-4908-6661Chylen Setiyo Rini1https://orcid.org/0000-0001-6390-3934Asih Rahayu2https://orcid.org/0000-0002-8252-1701Miarsono Sigit3Dyah Widhowati4https://orcid.org/0000-0003-3800-339XFaculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, East Java, 60225, Indonesia.Technology of Medical Laboratory, Faculty of Health, University of Muhammadiyah Sidoarjo, East Java, Indonesia, 61261, Indonesia.Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, East Java, 60225, Indonesia.Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, East Java, 60225, Indonesia.Faculty of Veterinary Medicine, University of Wijaya Kusuma Surabaya, East Java, 60225, Indonesia.Background and Aim: Antimicrobial resistance is a global problem caused by extensive utilization of antibiotics that promote gene resistant among bacteria, including Staphylococcus aureus. This study aimed to analyze the potential effects of celery (Apium graveolens) extract as an antioxidant and antimicrobial agent against methicillin-resistant S. aureus (MRSA), in vitro and in vivo. Materials and Methods: Celery was extracted and tested against a MRSA isolate in vitro. The minimum inhibitory concentration (MIC) against MRSA for the celery extract (CE) was determined to be 0.1% and it was formulated into a cream. A total of 30 female Sprague Dawley rats were divided into five groups: Group 1, negative control; Group 2, positive control; Group 3, treated with 0.05% CE cream; Group 4, 0.1% CE cream; and Group 5, 0.2% CE cream. All animals in the groups were exposed to a full-thickness skin biopsy on the dorsal portion, and they were infected with 30 μL of 105 colony-forming units of the MRSA isolate. The treatment was administered twice a day for 7 days. The skin samples were collected on days 3 and 7 after the treatment. The skin tissue was examined histologically using hematoxylin and eosin, Gram staining, and immunohistochemistry against cytokeratin (CK)-17. Results: Results showed that 0.2% of CE cream was the best treatment for wounds infected with MRSA. CE (0.2%) cream increased skin reepithelialization, fibroblast proliferation, and CK-17 expression; it also decreased the percentage of wound area, inflammatory cell infiltration, and bacterial colonization in skin wound tissue compared to the other treatments (p≤0.05). Conclusion: This study demonstrated that celery could be utilized as an alternative herbal therapy against MRSA-associated skin infections.http://www.veterinaryworld.org/Vol.13/May-2020/5.pdfantimicrobialcelerycytokeratin-17methicillin-resistant staphylococcus aureuswound healing |
| spellingShingle | Yos Adi Prakoso Chylen Setiyo Rini Asih Rahayu Miarsono Sigit Dyah Widhowati Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus Veterinary World antimicrobial celery cytokeratin-17 methicillin-resistant staphylococcus aureus wound healing |
| title | Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus |
| title_full | Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus |
| title_fullStr | Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus |
| title_full_unstemmed | Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus |
| title_short | Celery (Apium graveolens) as a potential antibacterial agent and its effect on cytokeratin-17 and other healing promoters in skin wounds infected with methicillin-resistant Staphylococcus aureus |
| title_sort | celery apium graveolens as a potential antibacterial agent and its effect on cytokeratin 17 and other healing promoters in skin wounds infected with methicillin resistant staphylococcus aureus |
| topic | antimicrobial celery cytokeratin-17 methicillin-resistant staphylococcus aureus wound healing |
| url | http://www.veterinaryworld.org/Vol.13/May-2020/5.pdf |
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