The cyanobacterial circadian clock follows midday in vivo and in vitro
Circadian rhythms are biological oscillations that schedule daily changes in physiology. Outside the laboratory, circadian clocks do not generally free-run but are driven by daily cues whose timing varies with the seasons. The principles that determine how circadian clocks align to these external cy...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/23539 |
| _version_ | 1828107616578961408 |
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| author | Eugene Leypunskiy Jenny Lin Haneul Yoo UnJin Lee Aaron R Dinner Michael J Rust |
| author_facet | Eugene Leypunskiy Jenny Lin Haneul Yoo UnJin Lee Aaron R Dinner Michael J Rust |
| author_sort | Eugene Leypunskiy |
| collection | DOAJ |
| description | Circadian rhythms are biological oscillations that schedule daily changes in physiology. Outside the laboratory, circadian clocks do not generally free-run but are driven by daily cues whose timing varies with the seasons. The principles that determine how circadian clocks align to these external cycles are not well understood. Here, we report experimental platforms for driving the cyanobacterial circadian clock both in vivo and in vitro. We find that the phase of the circadian rhythm follows a simple scaling law in light-dark cycles, tracking midday across conditions with variable day length. The core biochemical oscillator comprised of the Kai proteins behaves similarly when driven by metabolic pulses in vitro, indicating that such dynamics are intrinsic to these proteins. We develop a general mathematical framework based on instantaneous transformation of the clock cycle by external cues, which successfully predicts clock behavior under many cycling environments. |
| first_indexed | 2024-04-11T10:34:00Z |
| format | Article |
| id | doaj.art-d1928081f41a4f49bd7a65f1be988dd8 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:34:00Z |
| publishDate | 2017-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-d1928081f41a4f49bd7a65f1be988dd82022-12-22T04:29:21ZengeLife Sciences Publications LtdeLife2050-084X2017-07-01610.7554/eLife.23539The cyanobacterial circadian clock follows midday in vivo and in vitroEugene Leypunskiy0https://orcid.org/0000-0002-3335-1099Jenny Lin1Haneul Yoo2UnJin Lee3Aaron R Dinner4https://orcid.org/0000-0001-8328-6427Michael J Rust5https://orcid.org/0000-0002-7207-4020Graduate Program in Biophysical Sciences, The University of Chicago, Chicago, United StatesDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, United StatesDepartment of Biochemistry and Molecular Biology, The University of Chicago, Chicago, United StatesDepartment of Ecology and Evolution, The University of Chicago, Chicago, United StatesGraduate Program in Biophysical Sciences, The University of Chicago, Chicago, United States; Department of Chemistry, The University of Chicago, Chicago, United States; James Franck Institute, The University of Chicago, Chicago, United StatesGraduate Program in Biophysical Sciences, The University of Chicago, Chicago, United States; Department of Ecology and Evolution, The University of Chicago, Chicago, United States; Department of Molecular Genetics and Cell Biology, The University of Chicago, Chicago, United States; Department of Physics, The University of Chicago, Chicago, United StatesCircadian rhythms are biological oscillations that schedule daily changes in physiology. Outside the laboratory, circadian clocks do not generally free-run but are driven by daily cues whose timing varies with the seasons. The principles that determine how circadian clocks align to these external cycles are not well understood. Here, we report experimental platforms for driving the cyanobacterial circadian clock both in vivo and in vitro. We find that the phase of the circadian rhythm follows a simple scaling law in light-dark cycles, tracking midday across conditions with variable day length. The core biochemical oscillator comprised of the Kai proteins behaves similarly when driven by metabolic pulses in vitro, indicating that such dynamics are intrinsic to these proteins. We develop a general mathematical framework based on instantaneous transformation of the clock cycle by external cues, which successfully predicts clock behavior under many cycling environments.https://elifesciences.org/articles/23539cyanobacteriacircadiandynamical systems |
| spellingShingle | Eugene Leypunskiy Jenny Lin Haneul Yoo UnJin Lee Aaron R Dinner Michael J Rust The cyanobacterial circadian clock follows midday in vivo and in vitro eLife cyanobacteria circadian dynamical systems |
| title | The cyanobacterial circadian clock follows midday in vivo and in vitro |
| title_full | The cyanobacterial circadian clock follows midday in vivo and in vitro |
| title_fullStr | The cyanobacterial circadian clock follows midday in vivo and in vitro |
| title_full_unstemmed | The cyanobacterial circadian clock follows midday in vivo and in vitro |
| title_short | The cyanobacterial circadian clock follows midday in vivo and in vitro |
| title_sort | cyanobacterial circadian clock follows midday in vivo and in vitro |
| topic | cyanobacteria circadian dynamical systems |
| url | https://elifesciences.org/articles/23539 |
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