A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected].
DNA methylation is a key epigenetic regulator in all domains of life, yet the effects of most bacterial DNA methyltransferases on cellular processes are largely undefined. Here, we used diverse techniques, including bisulfite sequencing, transcriptomics, and transposon insertion site sequencing to e...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-11-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC4654547?pdf=render |
| _version_ | 1818158818345877504 |
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| author | Michael C Chao Shijia Zhu Satoshi Kimura Brigid M Davis Eric E Schadt Gang Fang Matthew K Waldor |
| author_facet | Michael C Chao Shijia Zhu Satoshi Kimura Brigid M Davis Eric E Schadt Gang Fang Matthew K Waldor |
| author_sort | Michael C Chao |
| collection | DOAJ |
| description | DNA methylation is a key epigenetic regulator in all domains of life, yet the effects of most bacterial DNA methyltransferases on cellular processes are largely undefined. Here, we used diverse techniques, including bisulfite sequencing, transcriptomics, and transposon insertion site sequencing to extensively characterize a 5-methylcytosine (5mC) methyltransferase, VchM, in the cholera pathogen, Vibrio cholerae. We have comprehensively defined VchM's DNA targets, its genetic interactions and the gene networks that it regulates. Although VchM is a relatively new component of the V. cholerae genome, it is required for optimal V. cholerae growth in vitro and during infection. Unexpectedly, the usually essential σE cell envelope stress pathway is dispensable in ∆vchM V. cholerae, likely due to its lower activation in this mutant and the capacity for VchM methylation to limit expression of some cell envelope modifying genes. Our work illuminates how an acquired DNA methyltransferase can become integrated within complex cell circuits to control critical housekeeping processes. |
| first_indexed | 2024-12-11T15:36:08Z |
| format | Article |
| id | doaj.art-d193114baf154a33b77d74901a0c4303 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-11T15:36:08Z |
| publishDate | 2015-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-d193114baf154a33b77d74901a0c43032022-12-22T00:59:57ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042015-11-011111e100566610.1371/journal.pgen.1005666A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected].Michael C ChaoShijia ZhuSatoshi KimuraBrigid M DavisEric E SchadtGang FangMatthew K WaldorDNA methylation is a key epigenetic regulator in all domains of life, yet the effects of most bacterial DNA methyltransferases on cellular processes are largely undefined. Here, we used diverse techniques, including bisulfite sequencing, transcriptomics, and transposon insertion site sequencing to extensively characterize a 5-methylcytosine (5mC) methyltransferase, VchM, in the cholera pathogen, Vibrio cholerae. We have comprehensively defined VchM's DNA targets, its genetic interactions and the gene networks that it regulates. Although VchM is a relatively new component of the V. cholerae genome, it is required for optimal V. cholerae growth in vitro and during infection. Unexpectedly, the usually essential σE cell envelope stress pathway is dispensable in ∆vchM V. cholerae, likely due to its lower activation in this mutant and the capacity for VchM methylation to limit expression of some cell envelope modifying genes. Our work illuminates how an acquired DNA methyltransferase can become integrated within complex cell circuits to control critical housekeeping processes.http://europepmc.org/articles/PMC4654547?pdf=render |
| spellingShingle | Michael C Chao Shijia Zhu Satoshi Kimura Brigid M Davis Eric E Schadt Gang Fang Matthew K Waldor A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected]. PLoS Genetics |
| title | A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected]. |
| title_full | A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected]. |
| title_fullStr | A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected]. |
| title_full_unstemmed | A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected]. |
| title_short | A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected]. |
| title_sort | cytosine methyltransferase modulates the cell envelope stress response in the cholera pathogen corrected |
| url | http://europepmc.org/articles/PMC4654547?pdf=render |
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