Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro
Abstract Background Sialic acids represent common terminal residues on numerous mammalian glycoconjugates, thereby influencing e.g. lumen formation in developing blood vessels. Interestingly, besides monosialylated also polysialylated glycoconjugates are produced by endothelial cells. Polysialic aci...
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | Cell & Bioscience |
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| Online Access: | http://link.springer.com/article/10.1186/s13578-018-0262-y |
| _version_ | 1828860605603250176 |
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| author | Sebastian Strubl Uwe Schubert Andrea Kühnle Alexander Rebl Negah Ahmadvand Silvia Fischer Klaus T. Preissner Sebastian P. Galuska |
| author_facet | Sebastian Strubl Uwe Schubert Andrea Kühnle Alexander Rebl Negah Ahmadvand Silvia Fischer Klaus T. Preissner Sebastian P. Galuska |
| author_sort | Sebastian Strubl |
| collection | DOAJ |
| description | Abstract Background Sialic acids represent common terminal residues on numerous mammalian glycoconjugates, thereby influencing e.g. lumen formation in developing blood vessels. Interestingly, besides monosialylated also polysialylated glycoconjugates are produced by endothelial cells. Polysialic acid (polySia) is formed in several organs during embryonal and postnatal development influencing, for instance, cell migration processes. Furthermore, the function of cytokines like basic fibroblast growth factor (bFGF) is modulated by polySia. Results In this study, we demonstrated that human umbilical vein endothelial cells (HUVEC) also secrete polysialylated glycoconjugates. Furthermore, an interaction between polySia and vascular endothelial growth factor (VEGF) was observed. VEGF modulates like bFGF the migration of HUVEC. Since both growth factors interact with polySia, we examined, if polySia modulates the migration of HUVEC. To this end scratch assays were performed showing that the migration of HUVEC is stimulated, when polySia was degraded. Conclusions Since polySia can interact with bFGF as well as VEGF and the degradation of polySia resulted in an increased cell migration capacity in the applied scratch assay, we propose that polySia may trap these growth factors influencing their biological activity. Thus, polySia might also contribute to the fine regulation of physiological processes in endothelial cells. |
| first_indexed | 2024-12-13T02:43:31Z |
| format | Article |
| id | doaj.art-d193c312727b4b15ae95bce8147d42b9 |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-12-13T02:43:31Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
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| series | Cell & Bioscience |
| spelling | doaj.art-d193c312727b4b15ae95bce8147d42b92022-12-22T00:02:15ZengBMCCell & Bioscience2045-37012018-12-01811910.1186/s13578-018-0262-yPolysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitroSebastian Strubl0Uwe Schubert1Andrea Kühnle2Alexander Rebl3Negah Ahmadvand4Silvia Fischer5Klaus T. Preissner6Sebastian P. Galuska7Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-UniversityInstitute of Biochemistry, Faculty of Medicine, Justus-Liebig-UniversityInstitute of Reproductive Biology, Leibniz Institute for Farm Animal Biology (FBN)Institute of Genome Biology, Leibniz Institute for Farm Animal Biology (FBN)Institute of Biochemistry, Faculty of Medicine, Justus-Liebig-UniversityInstitute of Biochemistry, Faculty of Medicine, Justus-Liebig-UniversityInstitute of Biochemistry, Faculty of Medicine, Justus-Liebig-UniversityInstitute of Biochemistry, Faculty of Medicine, Justus-Liebig-UniversityAbstract Background Sialic acids represent common terminal residues on numerous mammalian glycoconjugates, thereby influencing e.g. lumen formation in developing blood vessels. Interestingly, besides monosialylated also polysialylated glycoconjugates are produced by endothelial cells. Polysialic acid (polySia) is formed in several organs during embryonal and postnatal development influencing, for instance, cell migration processes. Furthermore, the function of cytokines like basic fibroblast growth factor (bFGF) is modulated by polySia. Results In this study, we demonstrated that human umbilical vein endothelial cells (HUVEC) also secrete polysialylated glycoconjugates. Furthermore, an interaction between polySia and vascular endothelial growth factor (VEGF) was observed. VEGF modulates like bFGF the migration of HUVEC. Since both growth factors interact with polySia, we examined, if polySia modulates the migration of HUVEC. To this end scratch assays were performed showing that the migration of HUVEC is stimulated, when polySia was degraded. Conclusions Since polySia can interact with bFGF as well as VEGF and the degradation of polySia resulted in an increased cell migration capacity in the applied scratch assay, we propose that polySia may trap these growth factors influencing their biological activity. Thus, polySia might also contribute to the fine regulation of physiological processes in endothelial cells.http://link.springer.com/article/10.1186/s13578-018-0262-yHuman umbilical vein endothelial cellsPolysialic acidVascular endothelial growth factor |
| spellingShingle | Sebastian Strubl Uwe Schubert Andrea Kühnle Alexander Rebl Negah Ahmadvand Silvia Fischer Klaus T. Preissner Sebastian P. Galuska Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro Cell & Bioscience Human umbilical vein endothelial cells Polysialic acid Vascular endothelial growth factor |
| title | Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro |
| title_full | Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro |
| title_fullStr | Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro |
| title_full_unstemmed | Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro |
| title_short | Polysialic acid is released by human umbilical vein endothelial cells (HUVEC) in vitro |
| title_sort | polysialic acid is released by human umbilical vein endothelial cells huvec in vitro |
| topic | Human umbilical vein endothelial cells Polysialic acid Vascular endothelial growth factor |
| url | http://link.springer.com/article/10.1186/s13578-018-0262-y |
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