Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma
Guanylate binding protein 5 (GBP5) is the interferon (IFN)-inducible subfamily of guanosine triphosphatases (GTPases) and is involved in pathogen defense. However, the role played by GBP5 in cancer development, especially in oral squamous cell carcinoma (OSCC), is still unknown. Herein, next-generat...
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| Format: | Article |
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MDPI AG
2021-08-01
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| Series: | Cancers |
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| Online Access: | https://www.mdpi.com/2072-6694/13/16/4043 |
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| author | Pei-Feng Liu Chih-Wen Shu Cheng-Hsin Lee Huei-Cin Sie Huei-Han Liou Jiin-Tsuey Cheng Luo-Ping Ger Chun-Lin Chen Chien-Chou Chen Chun-Feng Chen |
| author_facet | Pei-Feng Liu Chih-Wen Shu Cheng-Hsin Lee Huei-Cin Sie Huei-Han Liou Jiin-Tsuey Cheng Luo-Ping Ger Chun-Lin Chen Chien-Chou Chen Chun-Feng Chen |
| author_sort | Pei-Feng Liu |
| collection | DOAJ |
| description | Guanylate binding protein 5 (GBP5) is the interferon (IFN)-inducible subfamily of guanosine triphosphatases (GTPases) and is involved in pathogen defense. However, the role played by GBP5 in cancer development, especially in oral squamous cell carcinoma (OSCC), is still unknown. Herein, next-generation sequencing analysis showed that the gene expression levels of GBP5 were significantly higher in OSCC tissues compared with those found in corresponding tumor adjacent normal tissues (CTAN) from two pairs of OSCC patients. Higher gene expression levels of GBP5 were also found in tumor tissues of 23 buccal mucosal squamous cell carcinoma (BMSCC)/14 tongue squamous cell carcinoma (TSCC) patients and 30 oral cancer patients from The Cancer Genome Atlas (TCGA) database compared with those in CTAN tissues. Immunohistochemical results showed that protein expression levels of GBP5 were also higher in the tumor tissues of 353 OSCC patients including 117 BMSCC, 187 TSCC, and 49 lip squamous cell carcinoma patients. Moreover, TCGA database analysis indicated that high gene expression levels of GBP5 were associated with poor overall survival in oral cancer patients with moderate/poor cell differentiation, and associated with poor disease-free survival in oral cancer patients with moderate/poor cell differentiation and lymph node metastasis. Furthermore, GBP5-knockdowned cells exhibited decreased cell growth, arrest at G1 phase, and decreased invasion/migration. The gene expression of markers for epithelial−mesenchymal transition and cancer stemness was also reduced in GBP5-silenced oral cancer cells. Taken together, GBP5 might be a potential biomarker and therapeutic target for OSCC patients, especially for those with poor cell differentiation and lymph node metastasis. |
| first_indexed | 2024-03-10T08:57:54Z |
| format | Article |
| id | doaj.art-d19592e84db34859a640b52d86f93af4 |
| institution | Directory Open Access Journal |
| issn | 2072-6694 |
| language | English |
| last_indexed | 2024-03-10T08:57:54Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cancers |
| spelling | doaj.art-d19592e84db34859a640b52d86f93af42023-11-22T07:02:58ZengMDPI AGCancers2072-66942021-08-011316404310.3390/cancers13164043Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell CarcinomaPei-Feng Liu0Chih-Wen Shu1Cheng-Hsin Lee2Huei-Cin Sie3Huei-Han Liou4Jiin-Tsuey Cheng5Luo-Ping Ger6Chun-Lin Chen7Chien-Chou Chen8Chun-Feng Chen9Department of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, TaiwanInstitute of BioPharmaceutical Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDepartment of Biomedical Science and Environmental Biology, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, TaiwanDepartment of Pathology and Laboratory Medicine, Kaohsiung Veterans General Hospital, Kaohsiung 81362, TaiwanDepartment of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81362, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanDepartment of Medical Education and Research, Kaohsiung Veterans General Hospital, Kaohsiung 81362, TaiwanDepartment of Biological Sciences, National Sun Yat-sen University, Kaohsiung 80424, TaiwanFamily Medicine Division, Zuoying Branch of Kaohsiung Armed Forces General Hospital, Kaohsiung 81342, TaiwanDepartment of Stomatology, Kaohsiung Veterans General Hospital, Kaohsiung 81362, TaiwanGuanylate binding protein 5 (GBP5) is the interferon (IFN)-inducible subfamily of guanosine triphosphatases (GTPases) and is involved in pathogen defense. However, the role played by GBP5 in cancer development, especially in oral squamous cell carcinoma (OSCC), is still unknown. Herein, next-generation sequencing analysis showed that the gene expression levels of GBP5 were significantly higher in OSCC tissues compared with those found in corresponding tumor adjacent normal tissues (CTAN) from two pairs of OSCC patients. Higher gene expression levels of GBP5 were also found in tumor tissues of 23 buccal mucosal squamous cell carcinoma (BMSCC)/14 tongue squamous cell carcinoma (TSCC) patients and 30 oral cancer patients from The Cancer Genome Atlas (TCGA) database compared with those in CTAN tissues. Immunohistochemical results showed that protein expression levels of GBP5 were also higher in the tumor tissues of 353 OSCC patients including 117 BMSCC, 187 TSCC, and 49 lip squamous cell carcinoma patients. Moreover, TCGA database analysis indicated that high gene expression levels of GBP5 were associated with poor overall survival in oral cancer patients with moderate/poor cell differentiation, and associated with poor disease-free survival in oral cancer patients with moderate/poor cell differentiation and lymph node metastasis. Furthermore, GBP5-knockdowned cells exhibited decreased cell growth, arrest at G1 phase, and decreased invasion/migration. The gene expression of markers for epithelial−mesenchymal transition and cancer stemness was also reduced in GBP5-silenced oral cancer cells. Taken together, GBP5 might be a potential biomarker and therapeutic target for OSCC patients, especially for those with poor cell differentiation and lymph node metastasis.https://www.mdpi.com/2072-6694/13/16/4043oral squamous cell carcinomaguanylate binding protein 5malignancyprognosis |
| spellingShingle | Pei-Feng Liu Chih-Wen Shu Cheng-Hsin Lee Huei-Cin Sie Huei-Han Liou Jiin-Tsuey Cheng Luo-Ping Ger Chun-Lin Chen Chien-Chou Chen Chun-Feng Chen Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma Cancers oral squamous cell carcinoma guanylate binding protein 5 malignancy prognosis |
| title | Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma |
| title_full | Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma |
| title_fullStr | Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma |
| title_short | Clinical Significance and the Role of Guanylate-Binding Protein 5 in Oral Squamous Cell Carcinoma |
| title_sort | clinical significance and the role of guanylate binding protein 5 in oral squamous cell carcinoma |
| topic | oral squamous cell carcinoma guanylate binding protein 5 malignancy prognosis |
| url | https://www.mdpi.com/2072-6694/13/16/4043 |
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