Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities
To understand the mechanisms of immunomodulatory effect, Dendrobium Officinale polysaccharides (DOP) were treated by ultrasound and mild base separately to generate fractions of various weight-average molecular weight (Mw) and degrees of acetylation (DA). The structural features, conformational prop...
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| Format: | Article |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1016961/full |
| _version_ | 1811257832032436224 |
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| author | Xiaoyu Guo Mingguan Yang Changlu Wang Shaoping Nie Steve W. Cui Qingbin Guo |
| author_facet | Xiaoyu Guo Mingguan Yang Changlu Wang Shaoping Nie Steve W. Cui Qingbin Guo |
| author_sort | Xiaoyu Guo |
| collection | DOAJ |
| description | To understand the mechanisms of immunomodulatory effect, Dendrobium Officinale polysaccharides (DOP) were treated by ultrasound and mild base separately to generate fractions of various weight-average molecular weight (Mw) and degrees of acetylation (DA). The structural features, conformational properties, functional properties and immunomodulatory activities of original and modified DOPs were investigated. Ultrasonic treatment decreased the Mw and apparent viscosity and improved the water solubility of DOP. Mild base treatment remarkably reduced the DA and the water solubility, while the overall apparent viscosity was increased. Conformational analysis by triple-detector high performance size-exclusion chromatography showed that the molecular chain of DOP turned more compact coil conformation with decreased DA. Results from the macrophages RAW 264.7 analysis showed that samples sonicated for 200 min (Mw 34.2 kDa) showed the highest immune-regulation effects. However, the immunomodulatory effects of the samples after de-acetylation were all compromised compared to the original DOP. This study inspires further research to establish the structural-immunomodulatory relationships, which promote the application of DOP in both the food and medicine fields. |
| first_indexed | 2024-04-12T18:03:40Z |
| format | Article |
| id | doaj.art-d19723e26a3a439a861988599f3a43a8 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-12T18:03:40Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-d19723e26a3a439a861988599f3a43a82022-12-22T03:22:03ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.10169611016961Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activitiesXiaoyu Guo0Mingguan Yang1Changlu Wang2Shaoping Nie3Steve W. Cui4Qingbin Guo5State Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, ChinaCollege of Food Science and Engineering, Qilu University of Technology (Shandong Academy of Sciences), Jinan, ChinaState Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, ChinaState Key Laboratory of Food Science and Technology, China-Canada Joint Laboratory of Food Science and Technology (Nanchang), Nanchang University, Nanchang, ChinaGuelph Research and Development Centre, Agriculture and Agri-Food Canada, Guelph, ON, CanadaState Key Laboratory of Food Nutrition and Safety, College of Food Science and Technology, Tianjin University of Science and Technology, Tianjin, ChinaTo understand the mechanisms of immunomodulatory effect, Dendrobium Officinale polysaccharides (DOP) were treated by ultrasound and mild base separately to generate fractions of various weight-average molecular weight (Mw) and degrees of acetylation (DA). The structural features, conformational properties, functional properties and immunomodulatory activities of original and modified DOPs were investigated. Ultrasonic treatment decreased the Mw and apparent viscosity and improved the water solubility of DOP. Mild base treatment remarkably reduced the DA and the water solubility, while the overall apparent viscosity was increased. Conformational analysis by triple-detector high performance size-exclusion chromatography showed that the molecular chain of DOP turned more compact coil conformation with decreased DA. Results from the macrophages RAW 264.7 analysis showed that samples sonicated for 200 min (Mw 34.2 kDa) showed the highest immune-regulation effects. However, the immunomodulatory effects of the samples after de-acetylation were all compromised compared to the original DOP. This study inspires further research to establish the structural-immunomodulatory relationships, which promote the application of DOP in both the food and medicine fields.https://www.frontiersin.org/articles/10.3389/fnut.2022.1016961/fullDendrobium officinale polysaccharideO-acetylationimmunomodulatorystructural modificationglucomannan |
| spellingShingle | Xiaoyu Guo Mingguan Yang Changlu Wang Shaoping Nie Steve W. Cui Qingbin Guo Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities Frontiers in Nutrition Dendrobium officinale polysaccharide O-acetylation immunomodulatory structural modification glucomannan |
| title | Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities |
| title_full | Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities |
| title_fullStr | Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities |
| title_full_unstemmed | Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities |
| title_short | Acetyl-glucomannan from Dendrobium officinale: Structural modification and immunomodulatory activities |
| title_sort | acetyl glucomannan from dendrobium officinale structural modification and immunomodulatory activities |
| topic | Dendrobium officinale polysaccharide O-acetylation immunomodulatory structural modification glucomannan |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1016961/full |
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