Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves’ disease
Abstract Background B lymphocyte activating factor (BAFF) is a growth factor regulating B lymphocytes survival and maturation. Serum BAFF levels were elevated in patients affected with autoimmune thyroid diseases (AITD), including Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). The aim of thi...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-04-01
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| Series: | BMC Endocrine Disorders |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12902-021-00752-3 |
| _version_ | 1818598383757033472 |
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| author | Xin Wang Jinhui Huang Aixia Zhang Chen Fang Qi Ma Pengjun Jiang |
| author_facet | Xin Wang Jinhui Huang Aixia Zhang Chen Fang Qi Ma Pengjun Jiang |
| author_sort | Xin Wang |
| collection | DOAJ |
| description | Abstract Background B lymphocyte activating factor (BAFF) is a growth factor regulating B lymphocytes survival and maturation. Serum BAFF levels were elevated in patients affected with autoimmune thyroid diseases (AITD), including Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). The aim of this study is to explore the association of expression levels of BAFF and its receptors with AITD. Methods Fifty-two GD patients, 39 Hashimoto’s thyroiditis (HT) patients and 23 healthy controls (HC) were recruited in this study. Serum BAFF levels were measured by ELISA. Expression of BAFF receptors, including BAFF receptor 3 (BR3) and transmembrane activator and calcium-modulating and cyclophilin ligand interactor (TACI), on B lymphocytes were analyzed by flowcytometry. Effects of steroids on serum BAFF levels and expression of BR3 and TACI were also observed in 10 patients with Graves’ orbitopathy (GO) receiving steroids therapy. Results Serum BAFF levels were significantly elevated from 0.93 ± 0.24 ng/ml in HC to 1.18 ± 0.33 ng/ml in GD (P = 0.0027) and 1.02 ± 0.24 ng/ml in HT (P = 0.0331). BR3 expression on peripheral B lymphocytes were elevated in GD (mean MFI: 4.52 ± 2.06 in GD vs. 3.00 ± 0.87 in HC, P = 0.0015), while TACI expression on peripheral B lymphocytes were decreased in GD without significance (mean MFI: 7.96 ± 4.06 in GD vs. 9.10 ± 3.37 in HC, P = 0.1285). Expression of BR3 and TACI was not changed significantly in HT patients. Steroids significantly suppressed serum BAFF concentrations (from 1.18 ± 0.27 ng/ml to 0.97 ± 0.10 ng/ml, P = 0.0364) and BR3 expression in GO patients (mean MFI from 6.26 ± 4.91 to 4.05 ± 1.58, P = 0.0083). Conclusions Altered expression of BAFF and its receptor may mediate the autoimmunity in GD. Restoring the normal expression profile of receptors for BAFF could be a new strategy to treat GD. |
| first_indexed | 2024-12-16T12:02:50Z |
| format | Article |
| id | doaj.art-d198948878874d9abba5c1efe8839ffa |
| institution | Directory Open Access Journal |
| issn | 1472-6823 |
| language | English |
| last_indexed | 2024-12-16T12:02:50Z |
| publishDate | 2021-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Endocrine Disorders |
| spelling | doaj.art-d198948878874d9abba5c1efe8839ffa2022-12-21T22:32:24ZengBMCBMC Endocrine Disorders1472-68232021-04-012111610.1186/s12902-021-00752-3Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves’ diseaseXin Wang0Jinhui Huang1Aixia Zhang2Chen Fang3Qi Ma4Pengjun Jiang5Department of Endocrinology, Jiangsu Province Hospital of TCM/the Affiliated Hospital of Nanjing University of Chinese MedicineDepartment of Endocrinology, Jiangsu Province Hospital of TCM/the Affiliated Hospital of Nanjing University of Chinese MedicineSchool of Pharmacy, Nanjing Medical UniversityClinical Nutrition Department, Second affiliated Hospital of Soochow UniversityDepartment of Ultrasound, Second affiliated Hospital of Soochow UniversityDepartment of Hematology, Jiangsu Province Hospital of TCM/the Affiliated Hospital of Nanjing University of Chinese MedicineAbstract Background B lymphocyte activating factor (BAFF) is a growth factor regulating B lymphocytes survival and maturation. Serum BAFF levels were elevated in patients affected with autoimmune thyroid diseases (AITD), including Graves’ disease (GD) and Hashimoto’s thyroiditis (HT). The aim of this study is to explore the association of expression levels of BAFF and its receptors with AITD. Methods Fifty-two GD patients, 39 Hashimoto’s thyroiditis (HT) patients and 23 healthy controls (HC) were recruited in this study. Serum BAFF levels were measured by ELISA. Expression of BAFF receptors, including BAFF receptor 3 (BR3) and transmembrane activator and calcium-modulating and cyclophilin ligand interactor (TACI), on B lymphocytes were analyzed by flowcytometry. Effects of steroids on serum BAFF levels and expression of BR3 and TACI were also observed in 10 patients with Graves’ orbitopathy (GO) receiving steroids therapy. Results Serum BAFF levels were significantly elevated from 0.93 ± 0.24 ng/ml in HC to 1.18 ± 0.33 ng/ml in GD (P = 0.0027) and 1.02 ± 0.24 ng/ml in HT (P = 0.0331). BR3 expression on peripheral B lymphocytes were elevated in GD (mean MFI: 4.52 ± 2.06 in GD vs. 3.00 ± 0.87 in HC, P = 0.0015), while TACI expression on peripheral B lymphocytes were decreased in GD without significance (mean MFI: 7.96 ± 4.06 in GD vs. 9.10 ± 3.37 in HC, P = 0.1285). Expression of BR3 and TACI was not changed significantly in HT patients. Steroids significantly suppressed serum BAFF concentrations (from 1.18 ± 0.27 ng/ml to 0.97 ± 0.10 ng/ml, P = 0.0364) and BR3 expression in GO patients (mean MFI from 6.26 ± 4.91 to 4.05 ± 1.58, P = 0.0083). Conclusions Altered expression of BAFF and its receptor may mediate the autoimmunity in GD. Restoring the normal expression profile of receptors for BAFF could be a new strategy to treat GD.https://doi.org/10.1186/s12902-021-00752-3Graves’ diseaseHashimoto’s thyroiditisBAFFBR3TACI |
| spellingShingle | Xin Wang Jinhui Huang Aixia Zhang Chen Fang Qi Ma Pengjun Jiang Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves’ disease BMC Endocrine Disorders Graves’ disease Hashimoto’s thyroiditis BAFF BR3 TACI |
| title | Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves’ disease |
| title_full | Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves’ disease |
| title_fullStr | Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves’ disease |
| title_full_unstemmed | Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves’ disease |
| title_short | Altered expression profile of BAFF receptors on peripheral blood B lymphocytes in Graves’ disease |
| title_sort | altered expression profile of baff receptors on peripheral blood b lymphocytes in graves disease |
| topic | Graves’ disease Hashimoto’s thyroiditis BAFF BR3 TACI |
| url | https://doi.org/10.1186/s12902-021-00752-3 |
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