Distinct C4 sub‐types and C3 bundle sheath isolation in the Paniceae grasses
Abstract In C4 plants, the enzymatic machinery underpinning photosynthesis can vary, with, for example, three distinct C4 acid decarboxylases being used to release CO2 in the vicinity of RuBisCO. For decades, these decarboxylases have been used to classify C4 species into three biochemical sub‐types...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-12-01
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| Series: | Plant Direct |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/pld3.373 |
| _version_ | 1818363270542655488 |
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| author | Jacob D. Washburn Josh Strable Patrick Dickinson Satya S. Kothapalli Julia M. Brose Sarah Covshoff Gavin C. Conant Julian M. Hibberd Joseph Chris Pires |
| author_facet | Jacob D. Washburn Josh Strable Patrick Dickinson Satya S. Kothapalli Julia M. Brose Sarah Covshoff Gavin C. Conant Julian M. Hibberd Joseph Chris Pires |
| author_sort | Jacob D. Washburn |
| collection | DOAJ |
| description | Abstract In C4 plants, the enzymatic machinery underpinning photosynthesis can vary, with, for example, three distinct C4 acid decarboxylases being used to release CO2 in the vicinity of RuBisCO. For decades, these decarboxylases have been used to classify C4 species into three biochemical sub‐types. However, more recently, the notion that C4 species mix and match C4 acid decarboxylases has increased in popularity, and as a consequence, the validity of specific biochemical sub‐types has been questioned. Using five species from the grass tribe Paniceae, we show that, although in some species transcripts and enzymes involved in multiple C4 acid decarboxylases accumulate, in others, transcript abundance and enzyme activity is almost entirely from one decarboxylase. In addition, the development of a bundle sheath isolation procedure for a close C3 species in the Paniceae enables the preliminary exploration of C4 sub‐type evolution. |
| first_indexed | 2024-12-13T21:45:48Z |
| format | Article |
| id | doaj.art-d19b2cd095a94e898535d92836bce2fa |
| institution | Directory Open Access Journal |
| issn | 2475-4455 |
| language | English |
| last_indexed | 2024-12-13T21:45:48Z |
| publishDate | 2021-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Plant Direct |
| spelling | doaj.art-d19b2cd095a94e898535d92836bce2fa2022-12-21T23:30:26ZengWileyPlant Direct2475-44552021-12-01512n/an/a10.1002/pld3.373Distinct C4 sub‐types and C3 bundle sheath isolation in the Paniceae grassesJacob D. Washburn0Josh Strable1Patrick Dickinson2Satya S. Kothapalli3Julia M. Brose4Sarah Covshoff5Gavin C. Conant6Julian M. Hibberd7Joseph Chris Pires8Plant Genetics Research Unit, USDA‐ARS University of Missouri Columbia MO USADepartment of Molecular and Structural Biochemistry North Carolina State University Raleigh NC USADepartment of Plant Sciences University of Cambridge Cambridge UKDivision of Biological Sciences University of Missouri Columbia MO USADivision of Biological Sciences University of Missouri Columbia MO USADepartment of Plant Sciences University of Cambridge Cambridge UKProgram in Genetics, Bioinformatics Research Center, Department of Biological Sciences North Carolina State University Raleigh NC USADepartment of Plant Sciences University of Cambridge Cambridge UKDivision of Biological Sciences University of Missouri Columbia MO USAAbstract In C4 plants, the enzymatic machinery underpinning photosynthesis can vary, with, for example, three distinct C4 acid decarboxylases being used to release CO2 in the vicinity of RuBisCO. For decades, these decarboxylases have been used to classify C4 species into three biochemical sub‐types. However, more recently, the notion that C4 species mix and match C4 acid decarboxylases has increased in popularity, and as a consequence, the validity of specific biochemical sub‐types has been questioned. Using five species from the grass tribe Paniceae, we show that, although in some species transcripts and enzymes involved in multiple C4 acid decarboxylases accumulate, in others, transcript abundance and enzyme activity is almost entirely from one decarboxylase. In addition, the development of a bundle sheath isolation procedure for a close C3 species in the Paniceae enables the preliminary exploration of C4 sub‐type evolution.https://doi.org/10.1002/pld3.373C4C4 sub‐typesevolutionphotosynthesis |
| spellingShingle | Jacob D. Washburn Josh Strable Patrick Dickinson Satya S. Kothapalli Julia M. Brose Sarah Covshoff Gavin C. Conant Julian M. Hibberd Joseph Chris Pires Distinct C4 sub‐types and C3 bundle sheath isolation in the Paniceae grasses Plant Direct C4 C4 sub‐types evolution photosynthesis |
| title | Distinct C4 sub‐types and C3 bundle sheath isolation in the Paniceae grasses |
| title_full | Distinct C4 sub‐types and C3 bundle sheath isolation in the Paniceae grasses |
| title_fullStr | Distinct C4 sub‐types and C3 bundle sheath isolation in the Paniceae grasses |
| title_full_unstemmed | Distinct C4 sub‐types and C3 bundle sheath isolation in the Paniceae grasses |
| title_short | Distinct C4 sub‐types and C3 bundle sheath isolation in the Paniceae grasses |
| title_sort | distinct c4 sub types and c3 bundle sheath isolation in the paniceae grasses |
| topic | C4 C4 sub‐types evolution photosynthesis |
| url | https://doi.org/10.1002/pld3.373 |
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