Microencapsulation of DEET in Solid Lipid Microparticles: production, characterization and safety evaluation
Abstract DEET is considered the gold standard for insect repellent products. However, it behaves as a strong skin permeant. DEET was encapsulated in Solid Lipid Microparticles (SLM) and characterized in terms of morphology, particle size, cytotoxicity and ex vivo permeation. The particles exhibited...
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| Format: | Article |
| Language: | English |
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Academia Brasileira de Ciências
2022-09-01
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| Series: | Anais da Academia Brasileira de Ciências |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700702&lng=en&tlng=en |
| _version_ | 1811260318275338240 |
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| author | PAULA A. SANTOS MÁRCIO R.M. DA SILVA MELANIE TAVARES EDUARDO RICCI-JUNIOR |
| author_facet | PAULA A. SANTOS MÁRCIO R.M. DA SILVA MELANIE TAVARES EDUARDO RICCI-JUNIOR |
| author_sort | PAULA A. SANTOS |
| collection | DOAJ |
| description | Abstract DEET is considered the gold standard for insect repellent products. However, it behaves as a strong skin permeant. DEET was encapsulated in Solid Lipid Microparticles (SLM) and characterized in terms of morphology, particle size, cytotoxicity and ex vivo permeation. The particles exhibited micrometric size with a spherical shape. In addition, we developed and validated an analytical method for DEET quantification by high performance liquid chromatography (HPLC), which was selective, linear, precise, accurate and robust. The toxicity test in cell culture of keratinocytes, fibroblasts and macrophages showed that the formulation did not present cytotoxicity. The SLM were able to decrease the skin permeation of DEET in relation to the free active in ethanol with gain in the safe. Microparticles were able to increase the skin retention of DEET, which can contribute to extend the time of repellent action. The results showed that Solid Lipid Microparticles are safe and promising topical formulation to insect bite prevention. |
| first_indexed | 2024-04-12T18:45:39Z |
| format | Article |
| id | doaj.art-d19be5485c894227bd66c6ca6a3229b0 |
| institution | Directory Open Access Journal |
| issn | 1678-2690 |
| language | English |
| last_indexed | 2024-04-12T18:45:39Z |
| publishDate | 2022-09-01 |
| publisher | Academia Brasileira de Ciências |
| record_format | Article |
| series | Anais da Academia Brasileira de Ciências |
| spelling | doaj.art-d19be5485c894227bd66c6ca6a3229b02022-12-22T03:20:37ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências1678-26902022-09-0194suppl 310.1590/0001-3765202220211166Microencapsulation of DEET in Solid Lipid Microparticles: production, characterization and safety evaluationPAULA A. SANTOShttps://orcid.org/0000-0003-0423-7745MÁRCIO R.M. DA SILVAhttps://orcid.org/0000-0002-4261-6917MELANIE TAVAREShttps://orcid.org/0000-0003-1903-6751EDUARDO RICCI-JUNIORhttps://orcid.org/0000-0002-2550-696XAbstract DEET is considered the gold standard for insect repellent products. However, it behaves as a strong skin permeant. DEET was encapsulated in Solid Lipid Microparticles (SLM) and characterized in terms of morphology, particle size, cytotoxicity and ex vivo permeation. The particles exhibited micrometric size with a spherical shape. In addition, we developed and validated an analytical method for DEET quantification by high performance liquid chromatography (HPLC), which was selective, linear, precise, accurate and robust. The toxicity test in cell culture of keratinocytes, fibroblasts and macrophages showed that the formulation did not present cytotoxicity. The SLM were able to decrease the skin permeation of DEET in relation to the free active in ethanol with gain in the safe. Microparticles were able to increase the skin retention of DEET, which can contribute to extend the time of repellent action. The results showed that Solid Lipid Microparticles are safe and promising topical formulation to insect bite prevention.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700702&lng=en&tlng=enDEETSolid Lipid MicroparticlesEx vivo permeationCytotoxicity |
| spellingShingle | PAULA A. SANTOS MÁRCIO R.M. DA SILVA MELANIE TAVARES EDUARDO RICCI-JUNIOR Microencapsulation of DEET in Solid Lipid Microparticles: production, characterization and safety evaluation Anais da Academia Brasileira de Ciências DEET Solid Lipid Microparticles Ex vivo permeation Cytotoxicity |
| title | Microencapsulation of DEET in Solid Lipid Microparticles: production, characterization and safety evaluation |
| title_full | Microencapsulation of DEET in Solid Lipid Microparticles: production, characterization and safety evaluation |
| title_fullStr | Microencapsulation of DEET in Solid Lipid Microparticles: production, characterization and safety evaluation |
| title_full_unstemmed | Microencapsulation of DEET in Solid Lipid Microparticles: production, characterization and safety evaluation |
| title_short | Microencapsulation of DEET in Solid Lipid Microparticles: production, characterization and safety evaluation |
| title_sort | microencapsulation of deet in solid lipid microparticles production characterization and safety evaluation |
| topic | DEET Solid Lipid Microparticles Ex vivo permeation Cytotoxicity |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652022000700702&lng=en&tlng=en |
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