Time to Positivity Facilitates an Early Differential Diagnosis of Candida tropicalis from Other Candida species
Xianggui Yang,1,* Mingquan Liu,1,* Xuejing Yu,2 Zhenguo Wang,3 Ying Xu1 1Department of Laboratory Medicine, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, People’s Republic of China; 2Department of Internal Medicine, Division...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-10-01
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| Series: | Infection and Drug Resistance |
| Subjects: | |
| Online Access: | https://www.dovepress.com/time-to-positivity-facilitates-an-early-differential-diagnosis-of-cand-peer-reviewed-fulltext-article-IDR |
| _version_ | 1811194432627671040 |
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| author | Yang X Liu M Yu X Wang Z Xu Y |
| author_facet | Yang X Liu M Yu X Wang Z Xu Y |
| author_sort | Yang X |
| collection | DOAJ |
| description | Xianggui Yang,1,* Mingquan Liu,1,* Xuejing Yu,2 Zhenguo Wang,3 Ying Xu1 1Department of Laboratory Medicine, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, People’s Republic of China; 2Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA; 3Department of Stomatology, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xianggui Yang; Ying Xu, Tel/Fax +86-17358631230 ; +86-28-83016723, Email yxg204@163.com; yingxu@cmc.edu.cnBackground: Candidemia caused by Candida tropicalis has more serious adverse consequences and an even higher mortality. Time to positivity (TTP) has been widely used to identify microbial species, resistant microorganisms and distinguish real pathogens and pollutants. However, few studies have demonstrated TTP as a presumptive diagnosis of C. tropicalis in patients with candidemia.Patients and Methods: A retrospective study of 136 episodes of candidemia and simulated blood cultures with 314 episodes of confirmed Candida strains were applied to explore the role of TTPs in diagnosing C. tropicalis. TTPs were recorded as the shorter one if both aerobic and anaerobic vials were positive. Lastly, relationships were tested between TTPs and resistance and initial inocula concentration.Results: For the retrospective study, the mean of TTPs for C. tropicalis from 136 patients with candidemia was significantly shorter than other Candida species. The area under the receiver operating characteristics (ROC) curve was 0.8896 ± 0.030 with a sensitivity of 92.86% and a specificity of 77.87%, respectively, indicating TTPs with a cut-off value of < 25.50 h had a strong diagnostic power for C. tropicalis in patients with candidemia. Moreover, TTPs from 314 simulated blood cultures showed similar results as the retrospective study, demonstrating TTP is a powerful diagnostic tool in early diagnosing C. tropicalis in patients with candidemia. Additionally, our results showed no statistical significance between TTPs and initial inocula concentration and resistance of Candida species, suggesting initial inocula concentration does not impact TTPs, and TTPs may not be promising in predicting the resistance of all Candida species.Conclusion: TTP can be employed to early distinguish C. tropicalis from other Candida species in patients with candidemia, which is extremely helpful to initiate empiric antifungal treatments to improve clinical outcomes.Keywords: time to positivity, C. tropicalis, presumptive diagnosis, Candida species, candidemia |
| first_indexed | 2024-04-12T00:26:54Z |
| format | Article |
| id | doaj.art-d19dbad4a88d4e1ea54038731ea4c8b6 |
| institution | Directory Open Access Journal |
| issn | 1178-6973 |
| language | English |
| last_indexed | 2024-04-12T00:26:54Z |
| publishDate | 2022-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Infection and Drug Resistance |
| spelling | doaj.art-d19dbad4a88d4e1ea54038731ea4c8b62022-12-22T03:55:30ZengDove Medical PressInfection and Drug Resistance1178-69732022-10-01Volume 155879588678822Time to Positivity Facilitates an Early Differential Diagnosis of Candida tropicalis from Other Candida speciesYang XLiu MYu XWang ZXu YXianggui Yang,1,* Mingquan Liu,1,* Xuejing Yu,2 Zhenguo Wang,3 Ying Xu1 1Department of Laboratory Medicine, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, People’s Republic of China; 2Department of Internal Medicine, Division of Cardiology, University of Texas Southwestern Medical Center, Dallas, TX, USA; 3Department of Stomatology, Clinical Medical College and the First Affiliated Hospital of Chengdu Medical College, Chengdu, Sichuan, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xianggui Yang; Ying Xu, Tel/Fax +86-17358631230 ; +86-28-83016723, Email yxg204@163.com; yingxu@cmc.edu.cnBackground: Candidemia caused by Candida tropicalis has more serious adverse consequences and an even higher mortality. Time to positivity (TTP) has been widely used to identify microbial species, resistant microorganisms and distinguish real pathogens and pollutants. However, few studies have demonstrated TTP as a presumptive diagnosis of C. tropicalis in patients with candidemia.Patients and Methods: A retrospective study of 136 episodes of candidemia and simulated blood cultures with 314 episodes of confirmed Candida strains were applied to explore the role of TTPs in diagnosing C. tropicalis. TTPs were recorded as the shorter one if both aerobic and anaerobic vials were positive. Lastly, relationships were tested between TTPs and resistance and initial inocula concentration.Results: For the retrospective study, the mean of TTPs for C. tropicalis from 136 patients with candidemia was significantly shorter than other Candida species. The area under the receiver operating characteristics (ROC) curve was 0.8896 ± 0.030 with a sensitivity of 92.86% and a specificity of 77.87%, respectively, indicating TTPs with a cut-off value of < 25.50 h had a strong diagnostic power for C. tropicalis in patients with candidemia. Moreover, TTPs from 314 simulated blood cultures showed similar results as the retrospective study, demonstrating TTP is a powerful diagnostic tool in early diagnosing C. tropicalis in patients with candidemia. Additionally, our results showed no statistical significance between TTPs and initial inocula concentration and resistance of Candida species, suggesting initial inocula concentration does not impact TTPs, and TTPs may not be promising in predicting the resistance of all Candida species.Conclusion: TTP can be employed to early distinguish C. tropicalis from other Candida species in patients with candidemia, which is extremely helpful to initiate empiric antifungal treatments to improve clinical outcomes.Keywords: time to positivity, C. tropicalis, presumptive diagnosis, Candida species, candidemiahttps://www.dovepress.com/time-to-positivity-facilitates-an-early-differential-diagnosis-of-cand-peer-reviewed-fulltext-article-IDRtime to positivityc. tropicalispresumptive diagnosiscandida speciescandidemia |
| spellingShingle | Yang X Liu M Yu X Wang Z Xu Y Time to Positivity Facilitates an Early Differential Diagnosis of Candida tropicalis from Other Candida species Infection and Drug Resistance time to positivity c. tropicalis presumptive diagnosis candida species candidemia |
| title | Time to Positivity Facilitates an Early Differential Diagnosis of Candida tropicalis from Other Candida species |
| title_full | Time to Positivity Facilitates an Early Differential Diagnosis of Candida tropicalis from Other Candida species |
| title_fullStr | Time to Positivity Facilitates an Early Differential Diagnosis of Candida tropicalis from Other Candida species |
| title_full_unstemmed | Time to Positivity Facilitates an Early Differential Diagnosis of Candida tropicalis from Other Candida species |
| title_short | Time to Positivity Facilitates an Early Differential Diagnosis of Candida tropicalis from Other Candida species |
| title_sort | time to positivity facilitates an early differential diagnosis of candida tropicalis from other candida species |
| topic | time to positivity c. tropicalis presumptive diagnosis candida species candidemia |
| url | https://www.dovepress.com/time-to-positivity-facilitates-an-early-differential-diagnosis-of-cand-peer-reviewed-fulltext-article-IDR |
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