Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino Rats
Nephrotoxicity is one of the limiting factors for using doxorubicin (DOX). Honey, propolis, and royal jelly were evaluated for their ability to protect against nephrotoxicity caused by DOX. Forty-two adult albino rats were divided into control groups. The DOX group was injected i.p. with a weekly do...
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MDPI AG
2022-05-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/11/5/1029 |
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| author | Hanaa K. Mohamed Maysa A. Mobasher Rasha A. Ebiya Marwa T. Hassen Howaida M. Hagag Radwa El-Sayed Shaimaa Abdel-Ghany Manal M. Said Nabil S. Awad |
| author_facet | Hanaa K. Mohamed Maysa A. Mobasher Rasha A. Ebiya Marwa T. Hassen Howaida M. Hagag Radwa El-Sayed Shaimaa Abdel-Ghany Manal M. Said Nabil S. Awad |
| author_sort | Hanaa K. Mohamed |
| collection | DOAJ |
| description | Nephrotoxicity is one of the limiting factors for using doxorubicin (DOX). Honey, propolis, and royal jelly were evaluated for their ability to protect against nephrotoxicity caused by DOX. Forty-two adult albino rats were divided into control groups. The DOX group was injected i.p. with a weekly dose of 3 mg/kg of DOX for six weeks. The DOX plus honey treated group was injected with DOX and on the next day, received 500 mg/kg/day of honey orally for 21 days. The DOX plus royal jelly treated group was injected with DOX and on the following day, received 100 mg/kg/day of royal jelly orally for 21 days. The DOX plus propolis treated group received DOX and on the following day, was treated orally with 50 mg/kg/day of propolis for 21 days. The DOX plus combined treatment group received DOX and on the following day, was treated with a mix of honey, royal jelly, and propolis orally for 21 days. Results confirmed that DOX raised creatinine, urea, MDA, and TNF-α while decreasing GPX and SOD. Damages and elevated caspase-3 expression were discovered during renal tissue’s histopathological and immunohistochemical studies. Combined treatment with honey, royal jelly, and propolis improved biochemical, histological, and immunohistochemical studies in the renal tissue. qRT-PCR revealed increased expression of poly (ADP-Ribose) polymerase-1 (PARP-1) and a decline of Bcl-2 in the DOX group. However, combined treatment induced a significant decrease in the PARP-1 gene and increased Bcl-2 expression levels. In addition, the combined treatment led to significant improvement in the expression of both PARP-1 and Bcl-2 genes. In conclusion, the combined treatment effectively inhibited nephrotoxicity induced by DOX. |
| first_indexed | 2024-03-10T03:25:46Z |
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| id | doaj.art-d19ee399237d46428ee0c72ee83fbac8 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-10T03:25:46Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d19ee399237d46428ee0c72ee83fbac82023-11-23T09:53:18ZengMDPI AGAntioxidants2076-39212022-05-01115102910.3390/antiox11051029Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino RatsHanaa K. Mohamed0Maysa A. Mobasher1Rasha A. Ebiya2Marwa T. Hassen3Howaida M. Hagag4Radwa El-Sayed5Shaimaa Abdel-Ghany6Manal M. Said7Nabil S. Awad8Department of Zoology, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11757, EgyptDepartment of Pathology, Biochemistry Division, College of Medicine, Jouf University, Sakaka 41412, Saudi ArabiaDepartment of Zoology, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11757, EgyptDepartment of Zoology, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11757, EgyptDepartment of Pathology, Faculty of Medicine, Al-Azhar University, Nasr City, Cairo 11884, EgyptDepartment of Zoology, Faculty of Women for Arts, Science and Education, Ain Shams University, Cairo 11757, EgyptCollege of Biotechnology, Misr University for Science and Technology, Giza 12563, EgyptCollege of Biotechnology, Misr University for Science and Technology, Giza 12563, EgyptCollege of Biotechnology, Misr University for Science and Technology, Giza 12563, EgyptNephrotoxicity is one of the limiting factors for using doxorubicin (DOX). Honey, propolis, and royal jelly were evaluated for their ability to protect against nephrotoxicity caused by DOX. Forty-two adult albino rats were divided into control groups. The DOX group was injected i.p. with a weekly dose of 3 mg/kg of DOX for six weeks. The DOX plus honey treated group was injected with DOX and on the next day, received 500 mg/kg/day of honey orally for 21 days. The DOX plus royal jelly treated group was injected with DOX and on the following day, received 100 mg/kg/day of royal jelly orally for 21 days. The DOX plus propolis treated group received DOX and on the following day, was treated orally with 50 mg/kg/day of propolis for 21 days. The DOX plus combined treatment group received DOX and on the following day, was treated with a mix of honey, royal jelly, and propolis orally for 21 days. Results confirmed that DOX raised creatinine, urea, MDA, and TNF-α while decreasing GPX and SOD. Damages and elevated caspase-3 expression were discovered during renal tissue’s histopathological and immunohistochemical studies. Combined treatment with honey, royal jelly, and propolis improved biochemical, histological, and immunohistochemical studies in the renal tissue. qRT-PCR revealed increased expression of poly (ADP-Ribose) polymerase-1 (PARP-1) and a decline of Bcl-2 in the DOX group. However, combined treatment induced a significant decrease in the PARP-1 gene and increased Bcl-2 expression levels. In addition, the combined treatment led to significant improvement in the expression of both PARP-1 and Bcl-2 genes. In conclusion, the combined treatment effectively inhibited nephrotoxicity induced by DOX.https://www.mdpi.com/2076-3921/11/5/1029doxorubicinnephrotoxicityPARP-1Bcl-2TNF-αhoney |
| spellingShingle | Hanaa K. Mohamed Maysa A. Mobasher Rasha A. Ebiya Marwa T. Hassen Howaida M. Hagag Radwa El-Sayed Shaimaa Abdel-Ghany Manal M. Said Nabil S. Awad Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino Rats Antioxidants doxorubicin nephrotoxicity PARP-1 Bcl-2 TNF-α honey |
| title | Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino Rats |
| title_full | Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino Rats |
| title_fullStr | Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino Rats |
| title_full_unstemmed | Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino Rats |
| title_short | Anti-Inflammatory, Anti-Apoptotic, and Antioxidant Roles of Honey, Royal Jelly, and Propolis in Suppressing Nephrotoxicity Induced by Doxorubicin in Male Albino Rats |
| title_sort | anti inflammatory anti apoptotic and antioxidant roles of honey royal jelly and propolis in suppressing nephrotoxicity induced by doxorubicin in male albino rats |
| topic | doxorubicin nephrotoxicity PARP-1 Bcl-2 TNF-α honey |
| url | https://www.mdpi.com/2076-3921/11/5/1029 |
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