In vitro colonial inhibition of an isolate from Colletotrichum acutatum Simmonds to fungicide treatments
The aim of the research was to evaluate the in vitro sensitivity of Colletotrichum acutatum antracnosis to seven fungicides. It began with an isolate preserved in the ceparium of the Phytopathology Laboratory of the High School Studies of Xalostoc, Morelos. A completely randomized design was used to...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | Spanish |
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Universidad Central "Marta Abreu" de Las Villas
2018-02-01
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| Series: | Centro Agrícola |
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| Online Access: | http://cagricola.uclv.edu.cu/index.php/en/volume-44-2017/issue-4-2017/976-in-vitro-colonial-inhibition-of-an-isolate-from-colletotrichum-acutatum-simmonds-to-fungicide-treatments |
| _version_ | 1818172594493325312 |
|---|---|
| author | Dagoberto Guillén Sánchez Celeste Isamar Cadenas Vásquez Irán Alia Tejacal Víctor López Martínez María Andrade Rodríguez Porfirio Juárez López |
| author_facet | Dagoberto Guillén Sánchez Celeste Isamar Cadenas Vásquez Irán Alia Tejacal Víctor López Martínez María Andrade Rodríguez Porfirio Juárez López |
| author_sort | Dagoberto Guillén Sánchez |
| collection | DOAJ |
| description | The aim of the research was to evaluate the in vitro sensitivity of Colletotrichum acutatum antracnosis to seven fungicides. It began with an isolate preserved in the ceparium of the Phytopathology Laboratory of the High School Studies of Xalostoc, Morelos. A completely randomized design was used to evaluate the fungicides benomyl, diphenoconazole, azoxystrobin, trifloxystrobin, copper oxychloride, fluoxastrobin and captan, at high, medium and low doses, for a total of 22 treatments with six repetitions. Sterile distilled water was applied to the control. The treatments were applied at a rate of 5 mL per plate, which contained PDA medium and a mycelial disc (Ø 5 mm). The inoculated plates were incubated at 24 °C, in 12 hour photoperiod. The colony diameter was measured every 24 hours and the percent inhibition was calculated. A bifactorial variance analysis was performed, according to Fungicide and Dose; and the differences between treatments were detected by the LSD test with 95 % confidence. The diameter of the colony and the percentage of inhibition did not show differences for dose levels, without significant effects for the interaction of both factors, fungicides, and dose. However, all doses of the different fungicides inhibited colony growth compared to the control. The benomyl, difenoconazol and captan fungicides were able to totally reduce the growth of the C. acutatum colony; followed by copper oxychloride, azoxystrobin, fluoxastrobin and trifloxystrobin in decreasing order. Only benomyl, difenoconazol, captan and copper oxychloride achieved more than 50 % inhibition. |
| first_indexed | 2024-12-11T19:15:06Z |
| format | Article |
| id | doaj.art-d1a0943857714650a134b10505e69892 |
| institution | Directory Open Access Journal |
| issn | 0253-5785 2072-2001 |
| language | Spanish |
| last_indexed | 2024-12-11T19:15:06Z |
| publishDate | 2018-02-01 |
| publisher | Universidad Central "Marta Abreu" de Las Villas |
| record_format | Article |
| series | Centro Agrícola |
| spelling | doaj.art-d1a0943857714650a134b10505e698922022-12-22T00:53:40ZspaUniversidad Central "Marta Abreu" de Las VillasCentro Agrícola0253-57852072-20012018-02-014441116In vitro colonial inhibition of an isolate from Colletotrichum acutatum Simmonds to fungicide treatmentsDagoberto Guillén Sánchez0Celeste Isamar Cadenas Vásquez1Irán Alia Tejacal2Víctor López Martínez3María Andrade Rodríguez4Porfirio Juárez López5Universidad Autónoma del Estado de Morelos, Escuela de Estudios Superiores de XalostocUniversidad Autónoma del Estado de Morelos, Escuela de Estudios Superiores de XalostocUniversidad Autónoma del Estado de Morelos, Facultad de Ciencias AgropecuariasUniversidad Autónoma del Estado de Morelos, Facultad de Ciencias AgropecuariasUniversidad Autónoma del Estado de Morelos, Facultad de Ciencias AgropecuariasUniversidad Autónoma del Estado de Morelos, Facultad de Ciencias AgropecuariasThe aim of the research was to evaluate the in vitro sensitivity of Colletotrichum acutatum antracnosis to seven fungicides. It began with an isolate preserved in the ceparium of the Phytopathology Laboratory of the High School Studies of Xalostoc, Morelos. A completely randomized design was used to evaluate the fungicides benomyl, diphenoconazole, azoxystrobin, trifloxystrobin, copper oxychloride, fluoxastrobin and captan, at high, medium and low doses, for a total of 22 treatments with six repetitions. Sterile distilled water was applied to the control. The treatments were applied at a rate of 5 mL per plate, which contained PDA medium and a mycelial disc (Ø 5 mm). The inoculated plates were incubated at 24 °C, in 12 hour photoperiod. The colony diameter was measured every 24 hours and the percent inhibition was calculated. A bifactorial variance analysis was performed, according to Fungicide and Dose; and the differences between treatments were detected by the LSD test with 95 % confidence. The diameter of the colony and the percentage of inhibition did not show differences for dose levels, without significant effects for the interaction of both factors, fungicides, and dose. However, all doses of the different fungicides inhibited colony growth compared to the control. The benomyl, difenoconazol and captan fungicides were able to totally reduce the growth of the C. acutatum colony; followed by copper oxychloride, azoxystrobin, fluoxastrobin and trifloxystrobin in decreasing order. Only benomyl, difenoconazol, captan and copper oxychloride achieved more than 50 % inhibition.http://cagricola.uclv.edu.cu/index.php/en/volume-44-2017/issue-4-2017/976-in-vitro-colonial-inhibition-of-an-isolate-from-colletotrichum-acutatum-simmonds-to-fungicide-treatmentsanthracnosechemical controlorange |
| spellingShingle | Dagoberto Guillén Sánchez Celeste Isamar Cadenas Vásquez Irán Alia Tejacal Víctor López Martínez María Andrade Rodríguez Porfirio Juárez López In vitro colonial inhibition of an isolate from Colletotrichum acutatum Simmonds to fungicide treatments Centro Agrícola anthracnose chemical control orange |
| title | In vitro colonial inhibition of an isolate from Colletotrichum acutatum Simmonds to fungicide treatments |
| title_full | In vitro colonial inhibition of an isolate from Colletotrichum acutatum Simmonds to fungicide treatments |
| title_fullStr | In vitro colonial inhibition of an isolate from Colletotrichum acutatum Simmonds to fungicide treatments |
| title_full_unstemmed | In vitro colonial inhibition of an isolate from Colletotrichum acutatum Simmonds to fungicide treatments |
| title_short | In vitro colonial inhibition of an isolate from Colletotrichum acutatum Simmonds to fungicide treatments |
| title_sort | in vitro colonial inhibition of an isolate from colletotrichum acutatum simmonds to fungicide treatments |
| topic | anthracnose chemical control orange |
| url | http://cagricola.uclv.edu.cu/index.php/en/volume-44-2017/issue-4-2017/976-in-vitro-colonial-inhibition-of-an-isolate-from-colletotrichum-acutatum-simmonds-to-fungicide-treatments |
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