More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencing
Currently, lichen surveys are generally based on the examination of fruiting bodies. Lichens in the mycelial stage, in spores, or awaiting conditions for fruiting body formation are usually overlooked, even though they are important for maintaining biodiversity and ecosystem functions. This study ai...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.907531/full |
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| author | Benjawan Tanunchai Benjawan Tanunchai Simon Andreas Schroeter Li Ji Li Ji Sara Fareed Mohamed Wahdan Sara Fareed Mohamed Wahdan Shakhawat Hossen Shakhawat Hossen Ann-Sophie Lehnert Hagen Grünberg Gerd Gleixner François Buscot François Buscot Ernst-Detlef Schulze Matthias Noll Matthias Noll Witoon Purahong |
| author_facet | Benjawan Tanunchai Benjawan Tanunchai Simon Andreas Schroeter Li Ji Li Ji Sara Fareed Mohamed Wahdan Sara Fareed Mohamed Wahdan Shakhawat Hossen Shakhawat Hossen Ann-Sophie Lehnert Hagen Grünberg Gerd Gleixner François Buscot François Buscot Ernst-Detlef Schulze Matthias Noll Matthias Noll Witoon Purahong |
| author_sort | Benjawan Tanunchai |
| collection | DOAJ |
| description | Currently, lichen surveys are generally based on the examination of fruiting bodies. Lichens in the mycelial stage, in spores, or awaiting conditions for fruiting body formation are usually overlooked, even though they are important for maintaining biodiversity and ecosystem functions. This study aimed to explore the lichenized fungal community composition and richness associated with leaves and needles of 12 temperate tree species using Illumina MiSeq-based amplicon sequencing of the internal transcribed spacer (ITS) 2 region. Picea abies harbored the highest richness and number of lichenized fungal species. We found that the lichenized fungus Physcia adscendens dominated the leaves and needles of the most temperate tree species. Eleven lichenized fungal species detected in this study were recorded for the first time on leaves and needles. In addition, we identified Athallia cerinella, Fellhanera bouteillei, and Melanohalea exasperata that are on the German national red lists. Lichenized fungal richness was higher in conifer compared to broadleaf trees. Overall, tree species (within coniferous trees) and tree types (broadleaved vs. coniferous trees) harbored significantly different lichenized fungal community compositions pointing out the importance of host species. Diversity and community composition patterns of lichenized fungi were correlated mainly with tree species. Our study demonstrates that the diversity of foliicolous lichens associated with leaves and needles of 12 temperate tree species can be appropriately analyzed and functionally assigned using the ITS-based high-throughput sequencing. We highlighted the importance of conifers for maintaining the biodiversity of foliicolous lichens. Based on the discovery of many red list lichens, our methodological approach and results are important contributions to subsequent actions in the bio-conversation approaches. |
| first_indexed | 2024-04-12T19:07:04Z |
| format | Article |
| id | doaj.art-d1a0b895f124443ebc609f9c047829fa |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T19:07:04Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-d1a0b895f124443ebc609f9c047829fa2022-12-22T03:19:59ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-09-011310.3389/fmicb.2022.907531907531More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencingBenjawan Tanunchai0Benjawan Tanunchai1Simon Andreas Schroeter2Li Ji3Li Ji4Sara Fareed Mohamed Wahdan5Sara Fareed Mohamed Wahdan6Shakhawat Hossen7Shakhawat Hossen8Ann-Sophie Lehnert9Hagen Grünberg10Gerd Gleixner11François Buscot12François Buscot13Ernst-Detlef Schulze14Matthias Noll15Matthias Noll16Witoon Purahong17Department of Soil Ecology, UFZ-Helmholtz Centre for Environmental Research, Halle (Saale), GermanyBayreuth Center of Ecology and Environmental Research, University of Bayreuth, Bayreuth, GermanyBiogeochemical Processes Department, Max Planck Institute for Biogeochemistry, Jena, GermanyDepartment of Soil Ecology, UFZ-Helmholtz Centre for Environmental Research, Halle (Saale), GermanySchool of Forestry, Central South of Forestry and Technology, Changsha, ChinaDepartment of Soil Ecology, UFZ-Helmholtz Centre for Environmental Research, Halle (Saale), GermanyDepartment of Botany and Microbiology, Faculty of Science, Suez Canal University, Ismailia, EgyptDepartment of Soil Ecology, UFZ-Helmholtz Centre for Environmental Research, Halle (Saale), GermanyInstitute of Bioanalysis, Coburg University of Applied Sciences and Arts, Coburg, GermanyBiogeochemical Processes Department, Max Planck Institute for Biogeochemistry, Jena, GermanyPreßwitzer Straße, Unterwellenborn, GermanyBiogeochemical Processes Department, Max Planck Institute for Biogeochemistry, Jena, GermanyDepartment of Soil Ecology, UFZ-Helmholtz Centre for Environmental Research, Halle (Saale), GermanyGerman Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Leipzig, GermanyBiogeochemical Processes Department, Max Planck Institute for Biogeochemistry, Jena, GermanyBayreuth Center of Ecology and Environmental Research, University of Bayreuth, Bayreuth, GermanyInstitute of Bioanalysis, Coburg University of Applied Sciences and Arts, Coburg, GermanyDepartment of Soil Ecology, UFZ-Helmholtz Centre for Environmental Research, Halle (Saale), GermanyCurrently, lichen surveys are generally based on the examination of fruiting bodies. Lichens in the mycelial stage, in spores, or awaiting conditions for fruiting body formation are usually overlooked, even though they are important for maintaining biodiversity and ecosystem functions. This study aimed to explore the lichenized fungal community composition and richness associated with leaves and needles of 12 temperate tree species using Illumina MiSeq-based amplicon sequencing of the internal transcribed spacer (ITS) 2 region. Picea abies harbored the highest richness and number of lichenized fungal species. We found that the lichenized fungus Physcia adscendens dominated the leaves and needles of the most temperate tree species. Eleven lichenized fungal species detected in this study were recorded for the first time on leaves and needles. In addition, we identified Athallia cerinella, Fellhanera bouteillei, and Melanohalea exasperata that are on the German national red lists. Lichenized fungal richness was higher in conifer compared to broadleaf trees. Overall, tree species (within coniferous trees) and tree types (broadleaved vs. coniferous trees) harbored significantly different lichenized fungal community compositions pointing out the importance of host species. Diversity and community composition patterns of lichenized fungi were correlated mainly with tree species. Our study demonstrates that the diversity of foliicolous lichens associated with leaves and needles of 12 temperate tree species can be appropriately analyzed and functionally assigned using the ITS-based high-throughput sequencing. We highlighted the importance of conifers for maintaining the biodiversity of foliicolous lichens. Based on the discovery of many red list lichens, our methodological approach and results are important contributions to subsequent actions in the bio-conversation approaches.https://www.frontiersin.org/articles/10.3389/fmicb.2022.907531/fullITS2Physcia adscendensred-list lichenized fungifoliicolous lichensIllumina MiSeq |
| spellingShingle | Benjawan Tanunchai Benjawan Tanunchai Simon Andreas Schroeter Li Ji Li Ji Sara Fareed Mohamed Wahdan Sara Fareed Mohamed Wahdan Shakhawat Hossen Shakhawat Hossen Ann-Sophie Lehnert Hagen Grünberg Gerd Gleixner François Buscot François Buscot Ernst-Detlef Schulze Matthias Noll Matthias Noll Witoon Purahong More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencing Frontiers in Microbiology ITS2 Physcia adscendens red-list lichenized fungi foliicolous lichens Illumina MiSeq |
| title | More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencing |
| title_full | More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencing |
| title_fullStr | More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencing |
| title_full_unstemmed | More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencing |
| title_short | More than you can see: Unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high-throughput sequencing |
| title_sort | more than you can see unraveling the ecology and biodiversity of lichenized fungi associated with leaves and needles of 12 temperate tree species using high throughput sequencing |
| topic | ITS2 Physcia adscendens red-list lichenized fungi foliicolous lichens Illumina MiSeq |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.907531/full |
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