Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement
This study investigates the potential of formulated systems utilising haskap berry leaf extracts and dextran as carriers, to modulate both antioxidant and enzymatic inhibitory activities and their impact on the growth of specific bacterial strains. The analysis of antioxidant capacity, assessed thro...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-03-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/13/3/357 |
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| author | Szymon Sip Anna Sip Piotr Szulc Marek Selwet Marcin Żarowski Bogusław Czerny Judyta Cielecka-Piontek |
| author_facet | Szymon Sip Anna Sip Piotr Szulc Marek Selwet Marcin Żarowski Bogusław Czerny Judyta Cielecka-Piontek |
| author_sort | Szymon Sip |
| collection | DOAJ |
| description | This study investigates the potential of formulated systems utilising haskap berry leaf extracts and dextran as carriers, to modulate both antioxidant and enzymatic inhibitory activities and their impact on the growth of specific bacterial strains. The analysis of antioxidant capacity, assessed through ABTS, CUPRAC, DPPH, and FRAP assays, revealed varying but consistently high levels across extracts, with Extract 3 (loganic acid: 2.974 mg/g, chlorogenic acid: 1.125 mg/g, caffeic acid: 0.083 mg/g, rutin: 1.137 mg/g, and quercetin: 1.501 mg/g) exhibiting the highest values (ABTS: 0.2447 mg/mL, CUPRAC: 0.3121 mg/mL, DPPH: 0.21001 mg/mL, and FRAP: 0.3411 mg/mL). Subsequent enzymatic inhibition assays demonstrated a notable inhibitory potential against α-glucosidase (1.4915 mg/mL, expressed as acarbose equivalent), hyaluronidase (0.2982 mg/mL, expressed as quercetin equivalent), and lipase (5.8715 µg/mL, expressed as orlistat equivalent). Further system development involved integration with dextran, showcasing their preserved bioactive compound content and emphasising their stability and potential bioactivity. Evaluation of the dextran systems’ impact on bacterial growth revealed a significant proliferation of beneficial strains, particularly the <i>Bifidobacterium</i> and lactobacilli genus (Bifidobacterium longum: 9.54 × 10<sup>7</sup> to 1.57 × 10<sup>10</sup> CFU/mL and <i>Ligilactobacillus salivarius</i>: 1.36 × 10<sup>9</sup> to 1.62 × 10<sup>10</sup> CFU/mL), suggesting their potential to modulate gut microbiota. These findings offer a foundation for exploring the therapeutic applications of haskap berry-based dextran systems in managing conditions like diabetes, emphasising the interconnected roles of antioxidant-rich botanical extracts and dextran formulations in promoting overall metabolic health. |
| first_indexed | 2024-04-24T18:37:09Z |
| format | Article |
| id | doaj.art-d1a1428f847b4c23a8b178c7ad557f35 |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-04-24T18:37:09Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-d1a1428f847b4c23a8b178c7ad557f352024-03-27T13:18:41ZengMDPI AGAntioxidants2076-39212024-03-0113335710.3390/antiox13030357Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health EnhancementSzymon Sip0Anna Sip1Piotr Szulc2Marek Selwet3Marcin Żarowski4Bogusław Czerny5Judyta Cielecka-Piontek6Department of Pharmacognosy and Biomaterials, Faculty of Pharmacy, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznań, PolandDepartment of Biotechnology and Food Microbiology, Poznan University of Life Sciences, Wojska Polskiego 48, 60-627 Poznań, PolandDepartment of Agronomy, Poznań University of Life Sciences, Dojazd 11, 60-632 Poznań, PolandDepartment of Soil Science and Microbiology, Poznań University of Life Sciences, Szydłowska 50, 60-656 Poznań, PolandDepartment of Developmental Neurology, Poznan University of Medical Sciences, Przybyszewski 49, 60-355 Poznan, PolandDepartment of General Pharmacology and Pharmacoeconomics, Pomeranian Medical University in Szczecin, Żołnierska 48, 70-204 Szczecin, PolandDepartment of Pharmacognosy and Biomaterials, Faculty of Pharmacy, Poznań University of Medical Sciences, Rokietnicka 3, 60-806 Poznań, PolandThis study investigates the potential of formulated systems utilising haskap berry leaf extracts and dextran as carriers, to modulate both antioxidant and enzymatic inhibitory activities and their impact on the growth of specific bacterial strains. The analysis of antioxidant capacity, assessed through ABTS, CUPRAC, DPPH, and FRAP assays, revealed varying but consistently high levels across extracts, with Extract 3 (loganic acid: 2.974 mg/g, chlorogenic acid: 1.125 mg/g, caffeic acid: 0.083 mg/g, rutin: 1.137 mg/g, and quercetin: 1.501 mg/g) exhibiting the highest values (ABTS: 0.2447 mg/mL, CUPRAC: 0.3121 mg/mL, DPPH: 0.21001 mg/mL, and FRAP: 0.3411 mg/mL). Subsequent enzymatic inhibition assays demonstrated a notable inhibitory potential against α-glucosidase (1.4915 mg/mL, expressed as acarbose equivalent), hyaluronidase (0.2982 mg/mL, expressed as quercetin equivalent), and lipase (5.8715 µg/mL, expressed as orlistat equivalent). Further system development involved integration with dextran, showcasing their preserved bioactive compound content and emphasising their stability and potential bioactivity. Evaluation of the dextran systems’ impact on bacterial growth revealed a significant proliferation of beneficial strains, particularly the <i>Bifidobacterium</i> and lactobacilli genus (Bifidobacterium longum: 9.54 × 10<sup>7</sup> to 1.57 × 10<sup>10</sup> CFU/mL and <i>Ligilactobacillus salivarius</i>: 1.36 × 10<sup>9</sup> to 1.62 × 10<sup>10</sup> CFU/mL), suggesting their potential to modulate gut microbiota. These findings offer a foundation for exploring the therapeutic applications of haskap berry-based dextran systems in managing conditions like diabetes, emphasising the interconnected roles of antioxidant-rich botanical extracts and dextran formulations in promoting overall metabolic health.https://www.mdpi.com/2076-3921/13/3/357haskap berryprebiotic potentialantioxidantsby-productsdelivery systemsdextran |
| spellingShingle | Szymon Sip Anna Sip Piotr Szulc Marek Selwet Marcin Żarowski Bogusław Czerny Judyta Cielecka-Piontek Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement Antioxidants haskap berry prebiotic potential antioxidants by-products delivery systems dextran |
| title | Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement |
| title_full | Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement |
| title_fullStr | Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement |
| title_full_unstemmed | Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement |
| title_short | Exploring Beneficial Properties of Haskap Berry Leaf Compounds for Gut Health Enhancement |
| title_sort | exploring beneficial properties of haskap berry leaf compounds for gut health enhancement |
| topic | haskap berry prebiotic potential antioxidants by-products delivery systems dextran |
| url | https://www.mdpi.com/2076-3921/13/3/357 |
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