Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil
This research aims to immobilization; characterize the enzyme of immobilized, test the effectiveness of the enzyme of immobilized. This research begins with the immobilization to process of enzyme lipase using activated carbon matrix, enzyme characterization covering of immobile determination of tem...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Department of Chemistry, Pattimura University
2017-07-01
|
| Series: | Indonesian Journal of Chemical Research |
| Subjects: | |
| Online Access: | https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/158 |
| _version_ | 1818564304978313216 |
|---|---|
| author | Firdaus Firdaus Seniwati Dali Hendra J. Rusman |
| author_facet | Firdaus Firdaus Seniwati Dali Hendra J. Rusman |
| author_sort | Firdaus Firdaus |
| collection | DOAJ |
| description | This research aims to immobilization; characterize the enzyme of immobilized, test the effectiveness of the enzyme of immobilized. This research begins with the immobilization to process of enzyme lipase using activated carbon matrix, enzyme characterization covering of immobile determination of temperature and pH optimum of the enzyme of immobilized, as well as test the stability of work covering immobilized of enzyme the test thermal stability and repeated use. The results showed that the immobile of enzyme work optimally at 50oC of temperature and pH 6.5 with each activity 0.040 U/mL; research results also showed that the immobile of enzyme has higher thermal stability in comparison with the free enzyme: with the relative activity of 57.50% at the time of 45 minutes of exposure and the exposure time at 47.50% at 75-105 minutes and it can be used as many as six times with the relative activity of 52.5% in 6 times of use. |
| first_indexed | 2024-12-14T01:27:19Z |
| format | Article |
| id | doaj.art-d1a3b75b4ae64b30959e9f1a88cd9120 |
| institution | Directory Open Access Journal |
| issn | 2338-5359 2614-2627 |
| language | English |
| last_indexed | 2024-12-14T01:27:19Z |
| publishDate | 2017-07-01 |
| publisher | Department of Chemistry, Pattimura University |
| record_format | Article |
| series | Indonesian Journal of Chemical Research |
| spelling | doaj.art-d1a3b75b4ae64b30959e9f1a88cd91202022-12-21T23:22:08ZengDepartment of Chemistry, Pattimura UniversityIndonesian Journal of Chemical Research2338-53592614-26272017-07-015110.30598//ijcr.2017.5-firImobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim ImobilFirdaus Firdaus0Seniwati Dali1Hendra J. Rusman2Department Of Chemistry, Faculty Mathematics and Natural Science, Hasanuddin UniversityDepartment Of Chemistry, Faculty Mathematics and Natural Science, Hasanuddin UniversityDepartment Of Chemistry, Faculty Mathematics and Natural, Hasanuddin UniversityThis research aims to immobilization; characterize the enzyme of immobilized, test the effectiveness of the enzyme of immobilized. This research begins with the immobilization to process of enzyme lipase using activated carbon matrix, enzyme characterization covering of immobile determination of temperature and pH optimum of the enzyme of immobilized, as well as test the stability of work covering immobilized of enzyme the test thermal stability and repeated use. The results showed that the immobile of enzyme work optimally at 50oC of temperature and pH 6.5 with each activity 0.040 U/mL; research results also showed that the immobile of enzyme has higher thermal stability in comparison with the free enzyme: with the relative activity of 57.50% at the time of 45 minutes of exposure and the exposure time at 47.50% at 75-105 minutes and it can be used as many as six times with the relative activity of 52.5% in 6 times of use.https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/158Rice bran, lipase of enzymes, immobilization of enzymes, activated carbon, characterization, stability of the enzyme. |
| spellingShingle | Firdaus Firdaus Seniwati Dali Hendra J. Rusman Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil Indonesian Journal of Chemical Research Rice bran, lipase of enzymes, immobilization of enzymes, activated carbon, characterization, stability of the enzyme. |
| title | Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil |
| title_full | Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil |
| title_fullStr | Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil |
| title_full_unstemmed | Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil |
| title_short | Imobilisasi Enzim Lipase Dedak Padi (Oryza Sativa L.) Pada Karbon Aktif: Karakterisasi, dan Uji Stabilitas Kerja Enzim Imobil |
| title_sort | imobilisasi enzim lipase dedak padi oryza sativa l pada karbon aktif karakterisasi dan uji stabilitas kerja enzim imobil |
| topic | Rice bran, lipase of enzymes, immobilization of enzymes, activated carbon, characterization, stability of the enzyme. |
| url | https://ojs3.unpatti.ac.id/index.php/ijcr/article/view/158 |
| work_keys_str_mv | AT firdausfirdaus imobilisasienzimlipasededakpadioryzasativalpadakarbonaktifkarakterisasidanujistabilitaskerjaenzimimobil AT seniwatidali imobilisasienzimlipasededakpadioryzasativalpadakarbonaktifkarakterisasidanujistabilitaskerjaenzimimobil AT hendrajrusman imobilisasienzimlipasededakpadioryzasativalpadakarbonaktifkarakterisasidanujistabilitaskerjaenzimimobil |