Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains
Antibiotic therapy and its consequences in bacterial and human aspects are widely investigated. Despite this, the emergence of new multidrug resistant bacteria is still a current problem. The scope of our work included the observation of changes among uropathogenic Escherichia coli strains after the...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Virulence |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21505594.2019.1596507 |
| _version_ | 1818338206846812160 |
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| author | Wioletta Adamus-Białek Monika Wawszczak Michał Arabski Michał Majchrzak Martyna Gulba Dariusz Jarych Paweł Parniewski Stanisław Głuszek |
| author_facet | Wioletta Adamus-Białek Monika Wawszczak Michał Arabski Michał Majchrzak Martyna Gulba Dariusz Jarych Paweł Parniewski Stanisław Głuszek |
| author_sort | Wioletta Adamus-Białek |
| collection | DOAJ |
| description | Antibiotic therapy and its consequences in bacterial and human aspects are widely investigated. Despite this, the emergence of new multidrug resistant bacteria is still a current problem. The scope of our work included the observation of changes among uropathogenic Escherichia coli strains after the treatment with a subinhibitory concentration of different antibiotics. The sensitive strains with or without virulence factors were incubated with amoxicillin, ciprofloxacin, gentamycin, or tobramycin. After each passage, the E. coli derivatives were compared to their wild types based on their susceptibility profiles, virulence genes, biofilm formations and the fingerprint profiles of PCR products amplified with using the (N)(6)(CGG)(4) primer. It turned out that antibiotics caused significant changes in the repertoire of bacterial virulence and biofilm formation, corresponding to acquired cross-resistance. The genomic changes among the studied bacteria were reflected in the changed profiles of the CGG-PCR products. In conclusion, the inappropriate application of antibiotics may cause a rapid rise of Multidrug Resistant (MDR) strains and give bacteria a chance to modulate their own pathogenicity. This phenomenon has been easily observed among uropathogenic E. coli strains and it is one of the main reasons for recurrent infections of the urinary tract. |
| first_indexed | 2024-12-13T15:07:26Z |
| format | Article |
| id | doaj.art-d1a4697fabf145f290073bfb0cff4626 |
| institution | Directory Open Access Journal |
| issn | 2150-5594 2150-5608 |
| language | English |
| last_indexed | 2024-12-13T15:07:26Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Virulence |
| spelling | doaj.art-d1a4697fabf145f290073bfb0cff46262022-12-21T23:40:58ZengTaylor & Francis GroupVirulence2150-55942150-56082019-01-0110126027610.1080/21505594.2019.15965071596507Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strainsWioletta Adamus-Białek0Monika Wawszczak1Michał Arabski2Michał Majchrzak3Martyna Gulba4Dariusz Jarych5Paweł Parniewski6Stanisław Głuszek7Jan Kochanowski UniversityJan Kochanowski UniversityJan Kochanowski UniversityJan Kochanowski UniversityJan Kochanowski UniversityInstitute of Medical Biology, Polish Academy of SciencesInstitute of Medical Biology, Polish Academy of SciencesJan Kochanowski UniversityAntibiotic therapy and its consequences in bacterial and human aspects are widely investigated. Despite this, the emergence of new multidrug resistant bacteria is still a current problem. The scope of our work included the observation of changes among uropathogenic Escherichia coli strains after the treatment with a subinhibitory concentration of different antibiotics. The sensitive strains with or without virulence factors were incubated with amoxicillin, ciprofloxacin, gentamycin, or tobramycin. After each passage, the E. coli derivatives were compared to their wild types based on their susceptibility profiles, virulence genes, biofilm formations and the fingerprint profiles of PCR products amplified with using the (N)(6)(CGG)(4) primer. It turned out that antibiotics caused significant changes in the repertoire of bacterial virulence and biofilm formation, corresponding to acquired cross-resistance. The genomic changes among the studied bacteria were reflected in the changed profiles of the CGG-PCR products. In conclusion, the inappropriate application of antibiotics may cause a rapid rise of Multidrug Resistant (MDR) strains and give bacteria a chance to modulate their own pathogenicity. This phenomenon has been easily observed among uropathogenic E. coli strains and it is one of the main reasons for recurrent infections of the urinary tract.http://dx.doi.org/10.1080/21505594.2019.1596507uropathogenic escherichia coli strains (upec)antibioticsvirulence factor genesbiofilm |
| spellingShingle | Wioletta Adamus-Białek Monika Wawszczak Michał Arabski Michał Majchrzak Martyna Gulba Dariusz Jarych Paweł Parniewski Stanisław Głuszek Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains Virulence uropathogenic escherichia coli strains (upec) antibiotics virulence factor genes biofilm |
| title | Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains |
| title_full | Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains |
| title_fullStr | Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains |
| title_full_unstemmed | Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains |
| title_short | Ciprofloxacin, amoxicillin, and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic Escherichia coli strains |
| title_sort | ciprofloxacin amoxicillin and aminoglycosides stimulate genetic and phenotypic changes in uropathogenic escherichia coli strains |
| topic | uropathogenic escherichia coli strains (upec) antibiotics virulence factor genes biofilm |
| url | http://dx.doi.org/10.1080/21505594.2019.1596507 |
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