Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds
Invertase, the key enzyme responsible for sucrose hydrolysis. Inhibition of invertase can decrease the postprandial blood sugar level in diabetic patients and keep the blood glucose level normal where cytotoxicity to fast-growing cells like those of brine shrimp (Artemia salina) nauplii is a great m...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2020-06-01
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| Series: | Traditional and Integrative Medicine |
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| Online Access: | https://jtim.tums.ac.ir/index.php/jtim/article/view/232 |
| _version_ | 1818365412746723328 |
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| author | Asim Debnath Mohammad Salim Md Faruque Miah Md Rezaul Karim Md Jahangir Alam |
| author_facet | Asim Debnath Mohammad Salim Md Faruque Miah Md Rezaul Karim Md Jahangir Alam |
| author_sort | Asim Debnath |
| collection | DOAJ |
| description | Invertase, the key enzyme responsible for sucrose hydrolysis. Inhibition of invertase can decrease the postprandial blood sugar level in diabetic patients and keep the blood glucose level normal where cytotoxicity to fast-growing cells like those of brine shrimp (Artemia salina) nauplii is a great measurement for further important drug development like anticancer drugs. This study was aimed to investigate potential anti-diabetic and cytotoxic activities of the ethanol and acetone extracts of Swietenia macrophylla leaves, Syzygium cumini and Trigonella foenum-graecum seeds. Invertase inhibition activities of S. macrophylla leaves, S. cumini, and T. foenum-graecum seeds were measured by spectrophotometrically using standard protocols and cytotoxicities were measured by brine shrimp lethality bioassay. Among the plant extracts, all ethanol extracts showed higher invertase inhibition activities than all acetone extracts. S. cumini seed ethanol extract showed the highest invertase inhibition activity whereas S. macrophylla leaves acetone extract showed the lowest invertase inhibition activity. The maximum toxicity was observed in ethanol extract of T. foenum-graecum seed whereas the lowest toxicity was observed in acetone extract of S. macrophylla leaves. Both ethanol and acetone extract of T. foenum-graecum seeds showed significant cytotoxic activities. This investigation suggested that S. cumini and T. foenum-graecum seeds possess potential antidiabetic activities and T. foenum-graecum seeds have potential cytotoxicity. |
| first_indexed | 2024-12-13T22:19:51Z |
| format | Article |
| id | doaj.art-d1a512c265654ebaa89fc4892c6e2660 |
| institution | Directory Open Access Journal |
| issn | 2476-5104 2476-5112 |
| language | English |
| last_indexed | 2024-12-13T22:19:51Z |
| publishDate | 2020-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Traditional and Integrative Medicine |
| spelling | doaj.art-d1a512c265654ebaa89fc4892c6e26602022-12-21T23:29:24ZengTehran University of Medical SciencesTraditional and Integrative Medicine2476-51042476-51122020-06-015210.18502/tim.v5i2.3626Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum SeedsAsim Debnath0Mohammad Salim1Md Faruque Miah2Md Rezaul Karim3Md Jahangir Alam4Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, BangladeshDepartment of Chemistry, Shahjalal University of Science and Technology, Sylhet-3114, BangladeshDepartment of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, BangladeshBIRDEM General Hospital, Shahbagh Square, Dhaka-1000, Bangladesh1Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, Sylhet-3114, BangladeshInvertase, the key enzyme responsible for sucrose hydrolysis. Inhibition of invertase can decrease the postprandial blood sugar level in diabetic patients and keep the blood glucose level normal where cytotoxicity to fast-growing cells like those of brine shrimp (Artemia salina) nauplii is a great measurement for further important drug development like anticancer drugs. This study was aimed to investigate potential anti-diabetic and cytotoxic activities of the ethanol and acetone extracts of Swietenia macrophylla leaves, Syzygium cumini and Trigonella foenum-graecum seeds. Invertase inhibition activities of S. macrophylla leaves, S. cumini, and T. foenum-graecum seeds were measured by spectrophotometrically using standard protocols and cytotoxicities were measured by brine shrimp lethality bioassay. Among the plant extracts, all ethanol extracts showed higher invertase inhibition activities than all acetone extracts. S. cumini seed ethanol extract showed the highest invertase inhibition activity whereas S. macrophylla leaves acetone extract showed the lowest invertase inhibition activity. The maximum toxicity was observed in ethanol extract of T. foenum-graecum seed whereas the lowest toxicity was observed in acetone extract of S. macrophylla leaves. Both ethanol and acetone extract of T. foenum-graecum seeds showed significant cytotoxic activities. This investigation suggested that S. cumini and T. foenum-graecum seeds possess potential antidiabetic activities and T. foenum-graecum seeds have potential cytotoxicity.https://jtim.tums.ac.ir/index.php/jtim/article/view/232Plant extractsAntidiabetic activityInvertase inhibition activitCytotoxicity |
| spellingShingle | Asim Debnath Mohammad Salim Md Faruque Miah Md Rezaul Karim Md Jahangir Alam Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds Traditional and Integrative Medicine Plant extracts Antidiabetic activity Invertase inhibition activit Cytotoxicity |
| title | Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds |
| title_full | Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds |
| title_fullStr | Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds |
| title_full_unstemmed | Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds |
| title_short | Evaluation of Invertase Inhibition Activities and Cytotoxicity of Ethanol and Acetone Extracts of Swietenia macrophyllia Leaves, Syzygium cumini and Trigonella foenum-graecum Seeds |
| title_sort | evaluation of invertase inhibition activities and cytotoxicity of ethanol and acetone extracts of swietenia macrophyllia leaves syzygium cumini and trigonella foenum graecum seeds |
| topic | Plant extracts Antidiabetic activity Invertase inhibition activit Cytotoxicity |
| url | https://jtim.tums.ac.ir/index.php/jtim/article/view/232 |
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