Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 Analyzer – An Institutional Experience from North India.
Background: High performance liquid chromatography (HPLC) is the most commonly used method for detection and quantitative estimation of hemoglobin variants. Hemoglobinopathies are amongst the most common genetically inherited disorders, however, the exact magnitude of different hemoglobinopathies is...
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| Format: | Article |
| Language: | English |
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Light House Polyclinic Mangalore
2022-07-01
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| Series: | Online Journal of Health & Allied Sciences |
| Subjects: | |
| Online Access: | https://www.ojhas.org/issue82/2022-2-10.html |
| _version_ | 1811282534913277952 |
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| author | Shivali Sehgal Sabina Khan Sujata Jetley Yasir Alvi |
| author_facet | Shivali Sehgal Sabina Khan Sujata Jetley Yasir Alvi |
| author_sort | Shivali Sehgal |
| collection | DOAJ |
| description | Background: High performance liquid chromatography (HPLC) is the most commonly used method for detection and quantitative estimation of hemoglobin variants. Hemoglobinopathies are amongst the most common genetically inherited disorders, however, the exact magnitude of different hemoglobinopathies is obscure in India. This study was done with the aim of analyzing the different findings in HPLC using D-10 analyzer and evaluating the spectrum of different hemoglobin disorders in a hospital-based population of South Delhi. Such a prevalence study would be useful to review the various strategies that can be implemented for effective control and prevention of these disorders. Methods: A hospital based descriptive observational study was conducted in which all OPD and IPD patients who were advised HPLC during their clinical workup were included. Analysis of EDTA blood samples was done by Bio Rad D10 Dual program HPLC instrument. The exact percentage of HbA, HbA2, HbF and any other variant hemoglobin was estimated. Presumptive identification of hemoglobin variants was made primarily by their percentage, retention time (RT) and peak characteristics. HPLC findings were correlated with the clinical history, family history and the CBC and peripheral smear findings in all cases. Results: On HPLC analysis, 79% of the patients had no abnormality detected and the report was within normal limits. The commonest hemoglobinopathy was Beta Thalassemia Trait followed by HbE trait. The other hemoglobinopathies detected were HbD Punjab Heterozygous (3 cases, 0.5%), Beta thalassemia homozygous (3 cases, 0.5%), Sickle cell Heterozygous (2 cases, 0.3%), HbJ Meerut Heterozygous (2 cases, 0.3%). One case each of Sickle cell Homozygous (0.15%), Compound Heterozygous HbS/beta thalassemia trait (0.15%), HbE Homozygous (0.15%), Compound Heterozygous HbE/beta thalassemia trait (0.15%), and Homozygous delta beta thalassemia (0.15%) were also diagnosed. Conclusion: This study gives an important insight to the present day scenario of hemoglobinopathies in patients in South Delhi in relation to the hematological profile. It highlights the chromatogram findings of different hemoglobinopathies on the D10 analyzer. The comprehensive data obtained by such series can help in the formulation and development of infrastructure and policies for hemoglobinopathy prevention, diagnosis and management. |
| first_indexed | 2024-04-13T01:54:26Z |
| format | Article |
| id | doaj.art-d1a6d0ec610744139dfdbda2a5a73f63 |
| institution | Directory Open Access Journal |
| issn | 0972-5997 |
| language | English |
| last_indexed | 2024-04-13T01:54:26Z |
| publishDate | 2022-07-01 |
| publisher | Light House Polyclinic Mangalore |
| record_format | Article |
| series | Online Journal of Health & Allied Sciences |
| spelling | doaj.art-d1a6d0ec610744139dfdbda2a5a73f632022-12-22T03:07:48ZengLight House Polyclinic MangaloreOnline Journal of Health & Allied Sciences0972-59972022-07-01212Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 Analyzer – An Institutional Experience from North India.Shivali Sehgal0Sabina Khan1Sujata Jetley2Yasir Alvi3Department of Pathology, Hamdard Institute of Medical Sciences and Research, New Delhi.Department of Pathology, Hamdard Institute of Medical Sciences and Research, New Delhi.Department of Pathology, Hamdard Institute of Medical Sciences and Research, New Delhi.Department of Community Medicine, Hamdard Institute of Medical Sciences and Research, New Delhi.Background: High performance liquid chromatography (HPLC) is the most commonly used method for detection and quantitative estimation of hemoglobin variants. Hemoglobinopathies are amongst the most common genetically inherited disorders, however, the exact magnitude of different hemoglobinopathies is obscure in India. This study was done with the aim of analyzing the different findings in HPLC using D-10 analyzer and evaluating the spectrum of different hemoglobin disorders in a hospital-based population of South Delhi. Such a prevalence study would be useful to review the various strategies that can be implemented for effective control and prevention of these disorders. Methods: A hospital based descriptive observational study was conducted in which all OPD and IPD patients who were advised HPLC during their clinical workup were included. Analysis of EDTA blood samples was done by Bio Rad D10 Dual program HPLC instrument. The exact percentage of HbA, HbA2, HbF and any other variant hemoglobin was estimated. Presumptive identification of hemoglobin variants was made primarily by their percentage, retention time (RT) and peak characteristics. HPLC findings were correlated with the clinical history, family history and the CBC and peripheral smear findings in all cases. Results: On HPLC analysis, 79% of the patients had no abnormality detected and the report was within normal limits. The commonest hemoglobinopathy was Beta Thalassemia Trait followed by HbE trait. The other hemoglobinopathies detected were HbD Punjab Heterozygous (3 cases, 0.5%), Beta thalassemia homozygous (3 cases, 0.5%), Sickle cell Heterozygous (2 cases, 0.3%), HbJ Meerut Heterozygous (2 cases, 0.3%). One case each of Sickle cell Homozygous (0.15%), Compound Heterozygous HbS/beta thalassemia trait (0.15%), HbE Homozygous (0.15%), Compound Heterozygous HbE/beta thalassemia trait (0.15%), and Homozygous delta beta thalassemia (0.15%) were also diagnosed. Conclusion: This study gives an important insight to the present day scenario of hemoglobinopathies in patients in South Delhi in relation to the hematological profile. It highlights the chromatogram findings of different hemoglobinopathies on the D10 analyzer. The comprehensive data obtained by such series can help in the formulation and development of infrastructure and policies for hemoglobinopathy prevention, diagnosis and management.https://www.ojhas.org/issue82/2022-2-10.htmlhemoglobin varianthemoglobinopathyd-10 analyzernorth india |
| spellingShingle | Shivali Sehgal Sabina Khan Sujata Jetley Yasir Alvi Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 Analyzer – An Institutional Experience from North India. Online Journal of Health & Allied Sciences hemoglobin variant hemoglobinopathy d-10 analyzer north india |
| title | Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 Analyzer – An Institutional Experience from North India. |
| title_full | Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 Analyzer – An Institutional Experience from North India. |
| title_fullStr | Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 Analyzer – An Institutional Experience from North India. |
| title_full_unstemmed | Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 Analyzer – An Institutional Experience from North India. |
| title_short | Evaluation of Abnormal Hemoglobin Variants and Hemoglobinopathies on D-10 Analyzer – An Institutional Experience from North India. |
| title_sort | evaluation of abnormal hemoglobin variants and hemoglobinopathies on d 10 analyzer an institutional experience from north india |
| topic | hemoglobin variant hemoglobinopathy d-10 analyzer north india |
| url | https://www.ojhas.org/issue82/2022-2-10.html |
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