An immunohistochemical study and review of potential markers of human intestinal M cells
M cells are found in intestinal follicle associated epithelium. Studies into the physiological and pathological roles of human M cells have been hampered by the lack of well-substantiated, specific markers for these cells. A critical literature review suggests the following molecules may potentially...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2009-06-01
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| Series: | European Journal of Histochemistry |
| Online Access: | https://www.ejh.it/index.php/ejh/article/view/820 |
| _version_ | 1830282985484582912 |
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| author | NACS Wong M Herriot F Rae |
| author_facet | NACS Wong M Herriot F Rae |
| author_sort | NACS Wong |
| collection | DOAJ |
| description | M cells are found in intestinal follicle associated epithelium. Studies into the physiological and pathological roles of human M cells have been hampered by the lack of well-substantiated, specific markers for these cells. A critical literature review suggests the following molecules may potentially serve as such markers: CK7, FcaR (CD89), S100, CD1a, CD21, CD23, sialyl Lewis A, and cathepsin E. Normal ileum, appendix and colorectum were studied using paraffinembedded, formalin-fixed tissue and immunohistochemistry for these 8 markers. Cathepsin E immunohistochemistry was also performed on cases of colorectal adenocarcinoma, colorectal adenoma, colorectal hyperplastic/metaplastic polyp, lymphocytic colitis, collagenous colitis, pseudomembranous colitis and active ulcerative colitis. Of the 8 markers tested, only cathepsin E appeared to be specific to follicle associated epithelium (expressed by cells with and without M cell morphology) and follicular crypt epithelium; this specificity was limited to the colorectum. Focal epithelial expression of cathepsin E was seen in adenocarcinoma, adenoma, hyperplastic/metaplastic polyp, ulcerative colitis and pseudomembranous colitis. In conclusion, cathepsin E is a specific marker of normal colorectal follicle associated epithelium and follicular crypt epithelium though is not specific to M cells within these compartments. None of the other 7 markers studied is exclusively expressed by human M cells. |
| first_indexed | 2024-12-19T02:48:43Z |
| format | Article |
| id | doaj.art-d1a8ad270bed4616a71e2d50fd873b83 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-12-19T02:48:43Z |
| publishDate | 2009-06-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-d1a8ad270bed4616a71e2d50fd873b832022-12-21T20:38:46ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062009-06-0147210.4081/820555An immunohistochemical study and review of potential markers of human intestinal M cellsNACS WongM HerriotF RaeM cells are found in intestinal follicle associated epithelium. Studies into the physiological and pathological roles of human M cells have been hampered by the lack of well-substantiated, specific markers for these cells. A critical literature review suggests the following molecules may potentially serve as such markers: CK7, FcaR (CD89), S100, CD1a, CD21, CD23, sialyl Lewis A, and cathepsin E. Normal ileum, appendix and colorectum were studied using paraffinembedded, formalin-fixed tissue and immunohistochemistry for these 8 markers. Cathepsin E immunohistochemistry was also performed on cases of colorectal adenocarcinoma, colorectal adenoma, colorectal hyperplastic/metaplastic polyp, lymphocytic colitis, collagenous colitis, pseudomembranous colitis and active ulcerative colitis. Of the 8 markers tested, only cathepsin E appeared to be specific to follicle associated epithelium (expressed by cells with and without M cell morphology) and follicular crypt epithelium; this specificity was limited to the colorectum. Focal epithelial expression of cathepsin E was seen in adenocarcinoma, adenoma, hyperplastic/metaplastic polyp, ulcerative colitis and pseudomembranous colitis. In conclusion, cathepsin E is a specific marker of normal colorectal follicle associated epithelium and follicular crypt epithelium though is not specific to M cells within these compartments. None of the other 7 markers studied is exclusively expressed by human M cells.https://www.ejh.it/index.php/ejh/article/view/820 |
| spellingShingle | NACS Wong M Herriot F Rae An immunohistochemical study and review of potential markers of human intestinal M cells European Journal of Histochemistry |
| title | An immunohistochemical study and review of potential markers of human intestinal M cells |
| title_full | An immunohistochemical study and review of potential markers of human intestinal M cells |
| title_fullStr | An immunohistochemical study and review of potential markers of human intestinal M cells |
| title_full_unstemmed | An immunohistochemical study and review of potential markers of human intestinal M cells |
| title_short | An immunohistochemical study and review of potential markers of human intestinal M cells |
| title_sort | immunohistochemical study and review of potential markers of human intestinal m cells |
| url | https://www.ejh.it/index.php/ejh/article/view/820 |
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