Lipolytic System of the Tomato Pathogen Fusarium oxysporum f. sp. lycopersici
The lipolytic profile of Fusarium oxysporum f. sp lycopersici was studied by in silico search and biochemical enzyme activity analyses. Twenty-five structural secreted lipases were predicted based on the conserved pentapeptide Gly-X-Ser-X-Gly-, characteristic of fungal lipases, and secretion signal...
| मुख्य लेखकों: | , , |
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| स्वरूप: | लेख |
| भाषा: | English |
| प्रकाशित: |
The American Phytopathological Society
2013-09-01
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| श्रृंखला: | Molecular Plant-Microbe Interactions |
| ऑनलाइन पहुंच: | https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-13-0082-R |
| _version_ | 1828260147048218624 |
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| author | Gustavo Bravo-Ruiz Carmen Ruiz-Roldán M. Isabel G. Roncero |
| author_facet | Gustavo Bravo-Ruiz Carmen Ruiz-Roldán M. Isabel G. Roncero |
| author_sort | Gustavo Bravo-Ruiz |
| collection | DOAJ |
| description | The lipolytic profile of Fusarium oxysporum f. sp lycopersici was studied by in silico search and biochemical enzyme activity analyses. Twenty-five structural secreted lipases were predicted based on the conserved pentapeptide Gly-X-Ser-X-Gly-, characteristic of fungal lipases, and secretion signal sequences. Moreover, a predicted lipase regulatory gene was identified in addition to the previously characterized ctf1. The transcription profile of thirteen lipase genes during tomato plant colonization revealed that lip1, lip3, and lip22 were highly induced between 21 and 96 h after inoculation. Deletion mutants in five lipase genes (lip1, lip2, lip3, lip5, and lip22) and in the regulatory genes ctf1 and ctf2 as well as a Δctf1Δctf2 double mutant were generated. Quantitative reverse transcription-polymerase chain reaction expression analyses of structural lipase genes in the Δctf1, Δctf2, and Δctf1Δctf2 mutants indicated the existence of a complex lipase regulation network in F. oxysporum. The reduction of total lipase activity, as well as the severely reduced virulence of the Δctf1, Δctf2, and Δctf1Δctf2 mutants, provides evidence for an important role of the lipolytic system of this fungus in pathogenicity. |
| first_indexed | 2024-04-13T03:18:57Z |
| format | Article |
| id | doaj.art-d1accd401c9c4329b51a5d2f6d640a23 |
| institution | Directory Open Access Journal |
| issn | 0894-0282 1943-7706 |
| language | English |
| last_indexed | 2024-04-13T03:18:57Z |
| publishDate | 2013-09-01 |
| publisher | The American Phytopathological Society |
| record_format | Article |
| series | Molecular Plant-Microbe Interactions |
| spelling | doaj.art-d1accd401c9c4329b51a5d2f6d640a232022-12-22T03:04:50ZengThe American Phytopathological SocietyMolecular Plant-Microbe Interactions0894-02821943-77062013-09-012691054106710.1094/MPMI-03-13-0082-RLipolytic System of the Tomato Pathogen Fusarium oxysporum f. sp. lycopersiciGustavo Bravo-RuizCarmen Ruiz-RoldánM. Isabel G. RonceroThe lipolytic profile of Fusarium oxysporum f. sp lycopersici was studied by in silico search and biochemical enzyme activity analyses. Twenty-five structural secreted lipases were predicted based on the conserved pentapeptide Gly-X-Ser-X-Gly-, characteristic of fungal lipases, and secretion signal sequences. Moreover, a predicted lipase regulatory gene was identified in addition to the previously characterized ctf1. The transcription profile of thirteen lipase genes during tomato plant colonization revealed that lip1, lip3, and lip22 were highly induced between 21 and 96 h after inoculation. Deletion mutants in five lipase genes (lip1, lip2, lip3, lip5, and lip22) and in the regulatory genes ctf1 and ctf2 as well as a Δctf1Δctf2 double mutant were generated. Quantitative reverse transcription-polymerase chain reaction expression analyses of structural lipase genes in the Δctf1, Δctf2, and Δctf1Δctf2 mutants indicated the existence of a complex lipase regulation network in F. oxysporum. The reduction of total lipase activity, as well as the severely reduced virulence of the Δctf1, Δctf2, and Δctf1Δctf2 mutants, provides evidence for an important role of the lipolytic system of this fungus in pathogenicity.https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-13-0082-R |
| spellingShingle | Gustavo Bravo-Ruiz Carmen Ruiz-Roldán M. Isabel G. Roncero Lipolytic System of the Tomato Pathogen Fusarium oxysporum f. sp. lycopersici Molecular Plant-Microbe Interactions |
| title | Lipolytic System of the Tomato Pathogen Fusarium oxysporum f. sp. lycopersici |
| title_full | Lipolytic System of the Tomato Pathogen Fusarium oxysporum f. sp. lycopersici |
| title_fullStr | Lipolytic System of the Tomato Pathogen Fusarium oxysporum f. sp. lycopersici |
| title_full_unstemmed | Lipolytic System of the Tomato Pathogen Fusarium oxysporum f. sp. lycopersici |
| title_short | Lipolytic System of the Tomato Pathogen Fusarium oxysporum f. sp. lycopersici |
| title_sort | lipolytic system of the tomato pathogen fusarium oxysporum f sp lycopersici |
| url | https://apsjournals.apsnet.org/doi/10.1094/MPMI-03-13-0082-R |
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