Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers
Successful pregnancy establishment requires highly synchronized cross talk between the invasive trophoblast cells and the receptive maternal endometrium. Any disturbances in this tightly regulated process may lead to pregnancy complications. Local factors such as nutrients, hormones, cytokines and r...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/500 |
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| author | Andrea Pirković Milica Jovanović Krivokuća Aleksandra Vilotić Mirjana Nacka-Aleksić Žanka Bojić-Trbojević Dragana Dekanski |
| author_facet | Andrea Pirković Milica Jovanović Krivokuća Aleksandra Vilotić Mirjana Nacka-Aleksić Žanka Bojić-Trbojević Dragana Dekanski |
| author_sort | Andrea Pirković |
| collection | DOAJ |
| description | Successful pregnancy establishment requires highly synchronized cross talk between the invasive trophoblast cells and the receptive maternal endometrium. Any disturbances in this tightly regulated process may lead to pregnancy complications. Local factors such as nutrients, hormones, cytokines and reactive oxygen species modulate the invasion of extravillous trophoblasts through critical signaling cascades. Epidemiological studies strongly indicate that a Mediterranean diet can significantly impact molecular pathways during placentation. Therefore, the aim of the current study was to examine whether oleuropein (OLE), one of the main compounds of the Mediterranean diet, may influence trophoblast cell adhesion and migration, as well as the expression of invasion-associated molecular markers and inflammatory pathways fostering these processes. HTR-8/SVneo cells were incubated with OLE at selected concentrations of 10 and 100 µM for 24 h. Results showed that OLE did not affect trophoblast cell viability, proliferation and adhesion after 24 h in in vitro treatment. The mRNA expression of integrin subunits α1, α5 and β1, as well as matrix-degrading enzymes MMP-2 and -9, was significantly increased after treatment with 10 µM OLE. Furthermore, OLE at a concentration of 10 µM significantly increased the protein expression of integrin subunits α1 and β1. Also, OLE inhibited the activation of JNK and reduced the protein expression of COX-2. Finally, a lower concentration of OLE 10 µM significantly stimulated migration of HTR-8/SVneo cells. In conclusion, the obtained results demonstrate the effects of OLE on the function of trophoblast cells by promoting cell migration and stimulating the expression of invasion markers. As suggested from results, these effects may be mediated via inhibition of the JNK signaling pathway. |
| first_indexed | 2024-03-08T15:05:43Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:05:43Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-d1adab16163841ff98b0c1c9c8602e9a2024-01-10T14:59:45ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125150010.3390/ijms25010500Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated MarkersAndrea Pirković0Milica Jovanović Krivokuća1Aleksandra Vilotić2Mirjana Nacka-Aleksić3Žanka Bojić-Trbojević4Dragana Dekanski5Institute for the Application of Nuclear Energy, Department for Biology of Reproduction, University of Belgrade, Banatska 31b, 11080 Belgrade, SerbiaInstitute for the Application of Nuclear Energy, Department for Biology of Reproduction, University of Belgrade, Banatska 31b, 11080 Belgrade, SerbiaInstitute for the Application of Nuclear Energy, Department for Biology of Reproduction, University of Belgrade, Banatska 31b, 11080 Belgrade, SerbiaInstitute for the Application of Nuclear Energy, Department for Biology of Reproduction, University of Belgrade, Banatska 31b, 11080 Belgrade, SerbiaInstitute for the Application of Nuclear Energy, Department for Biology of Reproduction, University of Belgrade, Banatska 31b, 11080 Belgrade, SerbiaInstitute for the Application of Nuclear Energy, Department for Biology of Reproduction, University of Belgrade, Banatska 31b, 11080 Belgrade, SerbiaSuccessful pregnancy establishment requires highly synchronized cross talk between the invasive trophoblast cells and the receptive maternal endometrium. Any disturbances in this tightly regulated process may lead to pregnancy complications. Local factors such as nutrients, hormones, cytokines and reactive oxygen species modulate the invasion of extravillous trophoblasts through critical signaling cascades. Epidemiological studies strongly indicate that a Mediterranean diet can significantly impact molecular pathways during placentation. Therefore, the aim of the current study was to examine whether oleuropein (OLE), one of the main compounds of the Mediterranean diet, may influence trophoblast cell adhesion and migration, as well as the expression of invasion-associated molecular markers and inflammatory pathways fostering these processes. HTR-8/SVneo cells were incubated with OLE at selected concentrations of 10 and 100 µM for 24 h. Results showed that OLE did not affect trophoblast cell viability, proliferation and adhesion after 24 h in in vitro treatment. The mRNA expression of integrin subunits α1, α5 and β1, as well as matrix-degrading enzymes MMP-2 and -9, was significantly increased after treatment with 10 µM OLE. Furthermore, OLE at a concentration of 10 µM significantly increased the protein expression of integrin subunits α1 and β1. Also, OLE inhibited the activation of JNK and reduced the protein expression of COX-2. Finally, a lower concentration of OLE 10 µM significantly stimulated migration of HTR-8/SVneo cells. In conclusion, the obtained results demonstrate the effects of OLE on the function of trophoblast cells by promoting cell migration and stimulating the expression of invasion markers. As suggested from results, these effects may be mediated via inhibition of the JNK signaling pathway.https://www.mdpi.com/1422-0067/25/1/500oleuropeinpregnancytrophoblast functioncell migrationinvasion |
| spellingShingle | Andrea Pirković Milica Jovanović Krivokuća Aleksandra Vilotić Mirjana Nacka-Aleksić Žanka Bojić-Trbojević Dragana Dekanski Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers International Journal of Molecular Sciences oleuropein pregnancy trophoblast function cell migration invasion |
| title | Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers |
| title_full | Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers |
| title_fullStr | Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers |
| title_full_unstemmed | Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers |
| title_short | Oleuropein Stimulates Migration of Human Trophoblast Cells and Expression of Invasion-Associated Markers |
| title_sort | oleuropein stimulates migration of human trophoblast cells and expression of invasion associated markers |
| topic | oleuropein pregnancy trophoblast function cell migration invasion |
| url | https://www.mdpi.com/1422-0067/25/1/500 |
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