Value of Urease Test in the detection of Helicobacter pylori infections
Introduction: H. pylori is microaerophilic, garm negative, spiral organism with 4-6 flagella. The bacterium is an etiologic agent for chronic gastritis and plays a major role in effecting peptic ulcer, gastric cancer, lymphoma. H. pylori infection has a high seroprevalence in Iran (> 90%). Thus d...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Jahrom University of Medical Sciences
2005-04-01
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| Series: | فصلنامه دانشگاه علوم پزشکی جهرم |
| Subjects: | |
| Online Access: | http://jmj.jums.ac.ir/article-1-827-en.html |
| Summary: | Introduction: H. pylori is microaerophilic, garm negative, spiral organism with 4-6 flagella. The bacterium is an etiologic agent for chronic gastritis and plays a major role in effecting peptic ulcer, gastric cancer, lymphoma. H. pylori infection has a high seroprevalence in Iran (> 90%). Thus diagnosis and treatment of H.pylori infection is very important.
Material and Methods : The presence of H. pylori infection was determined in 100 patients undergoing upper gastrointestinal endoscopy over 9 month period. The presence of H. pyolri was detcted in gastric mucosal biopsy specimens by Biopsy Urease Test, PCR and histopathology. A media was used for urease test, Normal Broth Urea. The biopsy is placed in a broth containing urea and a PH indicator. The goldstandard is histopathology examination. We designed a PCR for amplifying the H. pylori gene encoding ure C (294 bp).
Results : The results of urease test were 66.6% sensitivity and 69.7% specefity. The results of PCR assay were 98.6% sensitivity and 93% specifity. There was a significant difference between the specifity and sensitivity of urease test and PCR.
Conclusion : The result of This study showed that urease test was not a sensitive and specific method for accurate detection of the infection. It a level of the sensitivity is too low to be used as the sole method of detection in ordinary practic. In this situation other/ tests should be used. |
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| ISSN: | 2008-7993 2008-8442 |