Biodegradation of Some Pesticides by Enterobacter cloacae; in vitro
The degradation of some pesticides (acetamiprid, boscalid, deltamethrin, kresoxim-methyl, pyraclostrobin and pyridaben) by Enterobacter cloacae (plant growth-promoting bacteria) was investigated in vitro. In this study, firstly the effective substances used initially did not have a negative effect o...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Turkish Science and Technology Publishing (TURSTEP)
2022-12-01
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| Series: | Turkish Journal of Agriculture: Food Science and Technology |
| Subjects: | |
| Online Access: | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5743 |
| _version_ | 1828021579893702656 |
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| author | Tarık Balkan Sabriye Belgüzar Mehmet Kızılarslan Özlem Yılmaz |
| author_facet | Tarık Balkan Sabriye Belgüzar Mehmet Kızılarslan Özlem Yılmaz |
| author_sort | Tarık Balkan |
| collection | DOAJ |
| description | The degradation of some pesticides (acetamiprid, boscalid, deltamethrin, kresoxim-methyl, pyraclostrobin and pyridaben) by Enterobacter cloacae (plant growth-promoting bacteria) was investigated in vitro. In this study, firstly the effective substances used initially did not have a negative effect on E. cloacae, and the bacteria showed the same growth as the control group in the nutrient agar medium. Secondly, application doses of pesticides were added to erlenmeyer with nutrient broth, and 1 ml of 1×109 cells/ml of E. cloacae solution was inoculated and incubated in a shaker at room temperature (24°C) for 7 days. After the nutrient broth medium was extracted, pesticides were measured by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). When the results were compared with the control group, there was no degradation in acetamiprid, but 98.9% in pyraclostrobin, 98.6% in boscalid, 94.1% in kresoxim-methyl, 73.5% in pyridaben and 57.3% in deltamethrin. It is thought that the results of this study and the information obtained on the degradation of boscalid, deltamethrin, kresoxim-methyl, pyraclostrobin and pyridaben will shed light on future studies. |
| first_indexed | 2024-04-10T11:56:15Z |
| format | Article |
| id | doaj.art-d1b37800d09d4e999c13b01b0f5ed087 |
| institution | Directory Open Access Journal |
| issn | 2148-127X |
| language | English |
| last_indexed | 2024-04-10T11:56:15Z |
| publishDate | 2022-12-01 |
| publisher | Turkish Science and Technology Publishing (TURSTEP) |
| record_format | Article |
| series | Turkish Journal of Agriculture: Food Science and Technology |
| spelling | doaj.art-d1b37800d09d4e999c13b01b0f5ed0872023-02-15T16:16:49ZengTurkish Science and Technology Publishing (TURSTEP)Turkish Journal of Agriculture: Food Science and Technology2148-127X2022-12-0110sp12783278810.24925/turjaf.v10isp1.2783-2788.57432740Biodegradation of Some Pesticides by Enterobacter cloacae; in vitroTarık Balkan0Sabriye Belgüzar1Mehmet Kızılarslan2Özlem Yılmaz3Department of Plant Protection, Faculty of Agriculture, Tokat Gaziosmanpasa University, TokatDepartment of Plant Protection, Faculty of Agriculture, Tokat Gaziosmanpasa University, TokatDepartment of Plant Protection, Faculty of Agriculture, Tokat Gaziosmanpasa University, TokatDepartment of Plant Protection, Faculty of Agriculture, Tokat Gaziosmanpasa University, TokatThe degradation of some pesticides (acetamiprid, boscalid, deltamethrin, kresoxim-methyl, pyraclostrobin and pyridaben) by Enterobacter cloacae (plant growth-promoting bacteria) was investigated in vitro. In this study, firstly the effective substances used initially did not have a negative effect on E. cloacae, and the bacteria showed the same growth as the control group in the nutrient agar medium. Secondly, application doses of pesticides were added to erlenmeyer with nutrient broth, and 1 ml of 1×109 cells/ml of E. cloacae solution was inoculated and incubated in a shaker at room temperature (24°C) for 7 days. After the nutrient broth medium was extracted, pesticides were measured by Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS). When the results were compared with the control group, there was no degradation in acetamiprid, but 98.9% in pyraclostrobin, 98.6% in boscalid, 94.1% in kresoxim-methyl, 73.5% in pyridaben and 57.3% in deltamethrin. It is thought that the results of this study and the information obtained on the degradation of boscalid, deltamethrin, kresoxim-methyl, pyraclostrobin and pyridaben will shed light on future studies.http://www.agrifoodscience.com/index.php/TURJAF/article/view/5743biyodegredasyonenterobacter cloacaepestisit kalıntısıpgprlc-ms/ms |
| spellingShingle | Tarık Balkan Sabriye Belgüzar Mehmet Kızılarslan Özlem Yılmaz Biodegradation of Some Pesticides by Enterobacter cloacae; in vitro Turkish Journal of Agriculture: Food Science and Technology biyodegredasyon enterobacter cloacae pestisit kalıntısı pgpr lc-ms/ms |
| title | Biodegradation of Some Pesticides by Enterobacter cloacae; in vitro |
| title_full | Biodegradation of Some Pesticides by Enterobacter cloacae; in vitro |
| title_fullStr | Biodegradation of Some Pesticides by Enterobacter cloacae; in vitro |
| title_full_unstemmed | Biodegradation of Some Pesticides by Enterobacter cloacae; in vitro |
| title_short | Biodegradation of Some Pesticides by Enterobacter cloacae; in vitro |
| title_sort | biodegradation of some pesticides by enterobacter cloacae in vitro |
| topic | biyodegredasyon enterobacter cloacae pestisit kalıntısı pgpr lc-ms/ms |
| url | http://www.agrifoodscience.com/index.php/TURJAF/article/view/5743 |
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