Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α
Light, an inevitable factor for human life, can have negative effects such as cellular inflammation and thus be toxic to cells. Lipids are important biomolecules that are involved in cellular inflammatory responses. Our research revealed the impact of light on cellular lipids. We discovered that blu...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-12-01
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| Series: | Journal of Photochemistry and Photobiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666469022000434 |
| _version_ | 1811202191413739520 |
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| author | Chi Zhang Lili Xu Mizuki Endo Tomoaki Kahyo Kenji Kikushima Makoto Horikawa Makoto Murakami A.S.M. Waliullah Md.Mahmudul Hasan Takumi Sakamoto Yutaka Takahashi Shuhei Aramaki Takeaki Ozawa Mitsutoshi Setou |
| author_facet | Chi Zhang Lili Xu Mizuki Endo Tomoaki Kahyo Kenji Kikushima Makoto Horikawa Makoto Murakami A.S.M. Waliullah Md.Mahmudul Hasan Takumi Sakamoto Yutaka Takahashi Shuhei Aramaki Takeaki Ozawa Mitsutoshi Setou |
| author_sort | Chi Zhang |
| collection | DOAJ |
| description | Light, an inevitable factor for human life, can have negative effects such as cellular inflammation and thus be toxic to cells. Lipids are important biomolecules that are involved in cellular inflammatory responses. Our research revealed the impact of light on cellular lipids. We discovered that blue light irradiation altered cellular lipidome based on non-targeted lipidomic analysis. Inflammation-related lysophospholipids, arachidonic acid, docosahexaenoic acid, and eicosapentaenoic acid, in particular, were significantly increased. We created opto-PiC, an optolipidomic tool based on the optogenetically engineered cPLA2 enzyme. Light-induced lipidomic changes were reversed by opto-PiC. Furthermore, the results showed that opto-PiC reduced cell death rate under blue light. Overall, our research provided lipidomic insights and optolipidomics approach into light toxicity. This could help the therapy development in the future for inflammation-related disease. |
| first_indexed | 2024-04-12T02:34:45Z |
| format | Article |
| id | doaj.art-d1b38504d6f9431c9041b512627361dd |
| institution | Directory Open Access Journal |
| issn | 2666-4690 |
| language | English |
| last_indexed | 2024-04-12T02:34:45Z |
| publishDate | 2022-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Photochemistry and Photobiology |
| spelling | doaj.art-d1b38504d6f9431c9041b512627361dd2022-12-22T03:51:36ZengElsevierJournal of Photochemistry and Photobiology2666-46902022-12-0112100150Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2αChi Zhang0Lili Xu1Mizuki Endo2Tomoaki Kahyo3Kenji Kikushima4Makoto Horikawa5Makoto Murakami6A.S.M. Waliullah7Md.Mahmudul Hasan8Takumi Sakamoto9Yutaka Takahashi10Shuhei Aramaki11Takeaki Ozawa12Mitsutoshi Setou13Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Chemistry, School of Science, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-0033, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Hiroshima Research Center for Healthy Aging, Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima, Hiroshima 739-8530, JapanGraduate School of Medicine, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-8655, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Department of Radiation Oncology, Hamamatsu University School of Medicine, Handayama, Higashi-ku, Hamamatsu, Shizuoka, Japan; Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, JapanDepartment of Chemistry, School of Science, The University of Tokyo, 7-3-1 Bunkyo-ku, Tokyo 113-0033, JapanDepartment of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; International Mass Imaging Center, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Department of Systems Molecular Anatomy, Institute for Medical Photonics Research, Preeminent Medical Photonics Education & Research Center, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan; Corresponding author at: Department of Cellular and Molecular Anatomy, Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku, Hamamatsu, Shizuoka 431-3192, Japan.Light, an inevitable factor for human life, can have negative effects such as cellular inflammation and thus be toxic to cells. Lipids are important biomolecules that are involved in cellular inflammatory responses. Our research revealed the impact of light on cellular lipids. We discovered that blue light irradiation altered cellular lipidome based on non-targeted lipidomic analysis. Inflammation-related lysophospholipids, arachidonic acid, docosahexaenoic acid, and eicosapentaenoic acid, in particular, were significantly increased. We created opto-PiC, an optolipidomic tool based on the optogenetically engineered cPLA2 enzyme. Light-induced lipidomic changes were reversed by opto-PiC. Furthermore, the results showed that opto-PiC reduced cell death rate under blue light. Overall, our research provided lipidomic insights and optolipidomics approach into light toxicity. This could help the therapy development in the future for inflammation-related disease.http://www.sciencedirect.com/science/article/pii/S2666469022000434LightLipidomicsInflammatory-related lipidsOptogenetic toolOptolipidomicscPLA2α |
| spellingShingle | Chi Zhang Lili Xu Mizuki Endo Tomoaki Kahyo Kenji Kikushima Makoto Horikawa Makoto Murakami A.S.M. Waliullah Md.Mahmudul Hasan Takumi Sakamoto Yutaka Takahashi Shuhei Aramaki Takeaki Ozawa Mitsutoshi Setou Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α Journal of Photochemistry and Photobiology Light Lipidomics Inflammatory-related lipids Optogenetic tool Optolipidomics cPLA2α |
| title | Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α |
| title_full | Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α |
| title_fullStr | Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α |
| title_full_unstemmed | Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α |
| title_short | Blue light alters cellular lipidome—Light-induced lipidomic changes can be modulated by optogenetically engineered cPLA2α |
| title_sort | blue light alters cellular lipidome light induced lipidomic changes can be modulated by optogenetically engineered cpla2α |
| topic | Light Lipidomics Inflammatory-related lipids Optogenetic tool Optolipidomics cPLA2α |
| url | http://www.sciencedirect.com/science/article/pii/S2666469022000434 |
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