Molecular Characterization of MYB Transcription Factors in <i>Camellia chekiangoleosa</i> Reveals That <i>CcMYB33</i> Is an Important Regulator Involved in Multiple Developmental Processes
<i>Camellia chekiangoleosa</i> is an economically important woody plant from the Genus <i>Camellia</i> in Theaceae, and its seed kernels are rich in edible oils of high health value. Yet, little is known about the molecular regulation of growth and development in <i>C....
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MDPI AG
2024-02-01
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| Series: | Horticulturae |
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| Online Access: | https://www.mdpi.com/2311-7524/10/2/178 |
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| author | Mingchuan Huang Sijia Li Minyan Wang Hengfu Yin Haimei Li |
| author_facet | Mingchuan Huang Sijia Li Minyan Wang Hengfu Yin Haimei Li |
| author_sort | Mingchuan Huang |
| collection | DOAJ |
| description | <i>Camellia chekiangoleosa</i> is an economically important woody plant from the Genus <i>Camellia</i> in Theaceae, and its seed kernels are rich in edible oils of high health value. Yet, little is known about the molecular regulation of growth and development in <i>C. chekiangoleosa</i>. In this study, we characterized the <i>MYB</i> (<i>Myeloblastosis</i>) gene family that was widely involved in plant development and stress responses, and identified 235 members from the <i>C. chekiangoleosa</i> genome. Based on transcriptomic analysis of multiple tissues, we obtained tissue-specific expression profiles of the <i>MYB</i> genes. We found that 37 <i>MYB</i> genes were highly expressed during seed development, and among them, <i>CcMYB33</i> (<i>GAMYB</i>) was specifically expressed in the seed coat, suggesting that it may be an important regulator. We cloned full-length sequences of the <i>CcMYB33</i> gene and further analyzed its sequence characteristics and expression pattern. Our results indicated that <i>CcMYB33</i> is an R2R3-type MYB transcription factor that is closely related to <i>GAMYB</i> genes of <i>Arabidopsis thaliana</i>. We showed that ectopic expression of <i>CcMYB33</i> in <i>Arabidopsis</i> lines caused pleiotropical developmental defects, including abnormal leaves, fused stamen, and early flowering, among other things. This work identified important MYB regulators in the regulation of development and growth in <i>C. chekiangoleosa</i>, providing support for further molecular and genetic studies. |
| first_indexed | 2024-03-07T22:31:00Z |
| format | Article |
| id | doaj.art-d1b4497bce9d481f84796a3fb46657b1 |
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| issn | 2311-7524 |
| language | English |
| last_indexed | 2024-03-07T22:31:00Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj.art-d1b4497bce9d481f84796a3fb46657b12024-02-23T15:18:49ZengMDPI AGHorticulturae2311-75242024-02-0110217810.3390/horticulturae10020178Molecular Characterization of MYB Transcription Factors in <i>Camellia chekiangoleosa</i> Reveals That <i>CcMYB33</i> Is an Important Regulator Involved in Multiple Developmental ProcessesMingchuan Huang0Sijia Li1Minyan Wang2Hengfu Yin3Haimei Li4College of Landscape Architecture and Forestry, Qingdao Agriculture University, Qingdao 266000, ChinaResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaResearch Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, ChinaCollege of Landscape Architecture and Forestry, Qingdao Agriculture University, Qingdao 266000, China<i>Camellia chekiangoleosa</i> is an economically important woody plant from the Genus <i>Camellia</i> in Theaceae, and its seed kernels are rich in edible oils of high health value. Yet, little is known about the molecular regulation of growth and development in <i>C. chekiangoleosa</i>. In this study, we characterized the <i>MYB</i> (<i>Myeloblastosis</i>) gene family that was widely involved in plant development and stress responses, and identified 235 members from the <i>C. chekiangoleosa</i> genome. Based on transcriptomic analysis of multiple tissues, we obtained tissue-specific expression profiles of the <i>MYB</i> genes. We found that 37 <i>MYB</i> genes were highly expressed during seed development, and among them, <i>CcMYB33</i> (<i>GAMYB</i>) was specifically expressed in the seed coat, suggesting that it may be an important regulator. We cloned full-length sequences of the <i>CcMYB33</i> gene and further analyzed its sequence characteristics and expression pattern. Our results indicated that <i>CcMYB33</i> is an R2R3-type MYB transcription factor that is closely related to <i>GAMYB</i> genes of <i>Arabidopsis thaliana</i>. We showed that ectopic expression of <i>CcMYB33</i> in <i>Arabidopsis</i> lines caused pleiotropical developmental defects, including abnormal leaves, fused stamen, and early flowering, among other things. This work identified important MYB regulators in the regulation of development and growth in <i>C. chekiangoleosa</i>, providing support for further molecular and genetic studies.https://www.mdpi.com/2311-7524/10/2/178<i>Camellia chekiangoleosa</i><i>CcMYB33</i>gene cloningexpression analysissubcellular localization |
| spellingShingle | Mingchuan Huang Sijia Li Minyan Wang Hengfu Yin Haimei Li Molecular Characterization of MYB Transcription Factors in <i>Camellia chekiangoleosa</i> Reveals That <i>CcMYB33</i> Is an Important Regulator Involved in Multiple Developmental Processes Horticulturae <i>Camellia chekiangoleosa</i> <i>CcMYB33</i> gene cloning expression analysis subcellular localization |
| title | Molecular Characterization of MYB Transcription Factors in <i>Camellia chekiangoleosa</i> Reveals That <i>CcMYB33</i> Is an Important Regulator Involved in Multiple Developmental Processes |
| title_full | Molecular Characterization of MYB Transcription Factors in <i>Camellia chekiangoleosa</i> Reveals That <i>CcMYB33</i> Is an Important Regulator Involved in Multiple Developmental Processes |
| title_fullStr | Molecular Characterization of MYB Transcription Factors in <i>Camellia chekiangoleosa</i> Reveals That <i>CcMYB33</i> Is an Important Regulator Involved in Multiple Developmental Processes |
| title_full_unstemmed | Molecular Characterization of MYB Transcription Factors in <i>Camellia chekiangoleosa</i> Reveals That <i>CcMYB33</i> Is an Important Regulator Involved in Multiple Developmental Processes |
| title_short | Molecular Characterization of MYB Transcription Factors in <i>Camellia chekiangoleosa</i> Reveals That <i>CcMYB33</i> Is an Important Regulator Involved in Multiple Developmental Processes |
| title_sort | molecular characterization of myb transcription factors in i camellia chekiangoleosa i reveals that i ccmyb33 i is an important regulator involved in multiple developmental processes |
| topic | <i>Camellia chekiangoleosa</i> <i>CcMYB33</i> gene cloning expression analysis subcellular localization |
| url | https://www.mdpi.com/2311-7524/10/2/178 |
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