Improvements in Simultaneous Sodium and Calcium Imaging
High speed imaging of ion concentration changes in neurons is an important and growing tool for neuroscientists. We previously developed a system for simultaneously measuring sodium and calcium changes in small compartments in neurons (Miyazaki and Ross, 2015). We used this technique to analyze the...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-01-01
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| Series: | Frontiers in Cellular Neuroscience |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fncel.2018.00514/full |
| _version_ | 1811320663091183616 |
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| author | Kenichi Miyazaki Kenichi Miyazaki John E. Lisman John E. Lisman William N. Ross William N. Ross |
| author_facet | Kenichi Miyazaki Kenichi Miyazaki John E. Lisman John E. Lisman William N. Ross William N. Ross |
| author_sort | Kenichi Miyazaki |
| collection | DOAJ |
| description | High speed imaging of ion concentration changes in neurons is an important and growing tool for neuroscientists. We previously developed a system for simultaneously measuring sodium and calcium changes in small compartments in neurons (Miyazaki and Ross, 2015). We used this technique to analyze the dynamics of these ions in individual pyramidal neuron dendritic spines (Miyazaki and Ross, 2017). This system is based on high speed multiplexing of light emitting diodes (LEDs) and classic organic indicators. To improve this system we made additional changes, primarily incorporating lasers in addition to the LEDs, more sophisticated imaging protocols, and the use of newer sodium and calcium indicators. This new system generates signals with higher signal to noise ratio (S/N), less background fluorescence, and less photodynamic damage. In addition, by using longer wavelength indicators instead of indicators sensitive in the UV range, it allows for the incorporation of focal uncaging along with simultaneous imaging, which should extend the range of experiments. |
| first_indexed | 2024-04-13T13:03:26Z |
| format | Article |
| id | doaj.art-d1b4cd4ef9f44c128c49923e40cee8ac |
| institution | Directory Open Access Journal |
| issn | 1662-5102 |
| language | English |
| last_indexed | 2024-04-13T13:03:26Z |
| publishDate | 2019-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular Neuroscience |
| spelling | doaj.art-d1b4cd4ef9f44c128c49923e40cee8ac2022-12-22T02:45:52ZengFrontiers Media S.A.Frontiers in Cellular Neuroscience1662-51022019-01-011210.3389/fncel.2018.00514427733Improvements in Simultaneous Sodium and Calcium ImagingKenichi Miyazaki0Kenichi Miyazaki1John E. Lisman2John E. Lisman3William N. Ross4William N. Ross5Department of Physiology, New York Medical College, Valhalla, NY, United StatesMarine Biological Laboratory, Woods Hole, MA, United StatesMarine Biological Laboratory, Woods Hole, MA, United StatesDepartment of Biology, Brandeis University, Waltham, MA, United StatesDepartment of Physiology, New York Medical College, Valhalla, NY, United StatesMarine Biological Laboratory, Woods Hole, MA, United StatesHigh speed imaging of ion concentration changes in neurons is an important and growing tool for neuroscientists. We previously developed a system for simultaneously measuring sodium and calcium changes in small compartments in neurons (Miyazaki and Ross, 2015). We used this technique to analyze the dynamics of these ions in individual pyramidal neuron dendritic spines (Miyazaki and Ross, 2017). This system is based on high speed multiplexing of light emitting diodes (LEDs) and classic organic indicators. To improve this system we made additional changes, primarily incorporating lasers in addition to the LEDs, more sophisticated imaging protocols, and the use of newer sodium and calcium indicators. This new system generates signals with higher signal to noise ratio (S/N), less background fluorescence, and less photodynamic damage. In addition, by using longer wavelength indicators instead of indicators sensitive in the UV range, it allows for the incorporation of focal uncaging along with simultaneous imaging, which should extend the range of experiments.https://www.frontiersin.org/article/10.3389/fncel.2018.00514/fullsodiumcalciumimagingdendritespinepyramidal neuron |
| spellingShingle | Kenichi Miyazaki Kenichi Miyazaki John E. Lisman John E. Lisman William N. Ross William N. Ross Improvements in Simultaneous Sodium and Calcium Imaging Frontiers in Cellular Neuroscience sodium calcium imaging dendrite spine pyramidal neuron |
| title | Improvements in Simultaneous Sodium and Calcium Imaging |
| title_full | Improvements in Simultaneous Sodium and Calcium Imaging |
| title_fullStr | Improvements in Simultaneous Sodium and Calcium Imaging |
| title_full_unstemmed | Improvements in Simultaneous Sodium and Calcium Imaging |
| title_short | Improvements in Simultaneous Sodium and Calcium Imaging |
| title_sort | improvements in simultaneous sodium and calcium imaging |
| topic | sodium calcium imaging dendrite spine pyramidal neuron |
| url | https://www.frontiersin.org/article/10.3389/fncel.2018.00514/full |
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