Development of a plant-produced recombinant monoclonal antibody against Δ-9-tetrahydrocannabinol (Δ9-THC) for immunoassay application
Δ-9-tetrahydrocannabinol (Δ9-THC) is mainly a psychoactive compound in the cannabis plant. The immunoassay, an alternative method to HPLC and GC, can be used to analyze and measure Δ9-THC. This method provides high sensitivity and specificity by using antibodies specific to the desired substances. C...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X2200025X |
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| author | Wanuttha Boonyayothin Khwanlada Kobtrakul Petlada Khositanon Sornkanok Vimolmangkang Waranyoo Phoolcharoen |
| author_facet | Wanuttha Boonyayothin Khwanlada Kobtrakul Petlada Khositanon Sornkanok Vimolmangkang Waranyoo Phoolcharoen |
| author_sort | Wanuttha Boonyayothin |
| collection | DOAJ |
| description | Δ-9-tetrahydrocannabinol (Δ9-THC) is mainly a psychoactive compound in the cannabis plant. The immunoassay, an alternative method to HPLC and GC, can be used to analyze and measure Δ9-THC. This method provides high sensitivity and specificity by using antibodies specific to the desired substances. Currently, plants provide several benefits over traditional expression platforms to produce recombinant antibodies, such as lower production costs and scalability. Therefore, this study aims to produce a recombinant anti-Δ9-THC monoclonal antibody (mAb) with transient expression using N. benthamiana. The highest expression level of the plant-produced mAb was estimated to be 0.33 ug/g leaf fresh weight. Our results demonstrate that the antibody provided in vitro affinity binding related to Δ9-THC and the metabolites of Δ9-THC, such as cannabinol (CBN). Moreover, the antibody also showed binding efficiency with Δ9-THC in cannabis extract. Moreover, plant-produced mAbs provide efficiency against Δ9-THC and can be applied for further immunoassay applications. |
| first_indexed | 2024-04-12T17:01:48Z |
| format | Article |
| id | doaj.art-d1b669c87ed6461c838e18a1d5fef681 |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-12T17:01:48Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-d1b669c87ed6461c838e18a1d5fef6812022-12-22T03:24:03ZengElsevierBiotechnology Reports2215-017X2022-06-0134e00725Development of a plant-produced recombinant monoclonal antibody against Δ-9-tetrahydrocannabinol (Δ9-THC) for immunoassay applicationWanuttha Boonyayothin0Khwanlada Kobtrakul1Petlada Khositanon2Sornkanok Vimolmangkang3Waranyoo Phoolcharoen4Center of Excellence in Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok, Thailand; Pharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, ThailandPharmaceutical Sciences and Technology Program, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, ThailandResearch Cluster for Cannabis and its Natural Substances, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; Research Cluster for Cannabis and its Natural Substances, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Plant-Produced Pharmaceuticals, Chulalongkorn University, Bangkok, Thailand; Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand; Corresponding author at: Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Chulalongkorn University, Bangkok, Thailand.Δ-9-tetrahydrocannabinol (Δ9-THC) is mainly a psychoactive compound in the cannabis plant. The immunoassay, an alternative method to HPLC and GC, can be used to analyze and measure Δ9-THC. This method provides high sensitivity and specificity by using antibodies specific to the desired substances. Currently, plants provide several benefits over traditional expression platforms to produce recombinant antibodies, such as lower production costs and scalability. Therefore, this study aims to produce a recombinant anti-Δ9-THC monoclonal antibody (mAb) with transient expression using N. benthamiana. The highest expression level of the plant-produced mAb was estimated to be 0.33 ug/g leaf fresh weight. Our results demonstrate that the antibody provided in vitro affinity binding related to Δ9-THC and the metabolites of Δ9-THC, such as cannabinol (CBN). Moreover, the antibody also showed binding efficiency with Δ9-THC in cannabis extract. Moreover, plant-produced mAbs provide efficiency against Δ9-THC and can be applied for further immunoassay applications.http://www.sciencedirect.com/science/article/pii/S2215017X2200025XPlant-produced mAbTransient expression(-)-Δ 9-tetrahydrocannabinol (Δ9-THC)Nicotiana benthamiana, Cannabis extractImmunoassay |
| spellingShingle | Wanuttha Boonyayothin Khwanlada Kobtrakul Petlada Khositanon Sornkanok Vimolmangkang Waranyoo Phoolcharoen Development of a plant-produced recombinant monoclonal antibody against Δ-9-tetrahydrocannabinol (Δ9-THC) for immunoassay application Biotechnology Reports Plant-produced mAb Transient expression (-)-Δ 9-tetrahydrocannabinol (Δ9-THC) Nicotiana benthamiana, Cannabis extract Immunoassay |
| title | Development of a plant-produced recombinant monoclonal antibody against Δ-9-tetrahydrocannabinol (Δ9-THC) for immunoassay application |
| title_full | Development of a plant-produced recombinant monoclonal antibody against Δ-9-tetrahydrocannabinol (Δ9-THC) for immunoassay application |
| title_fullStr | Development of a plant-produced recombinant monoclonal antibody against Δ-9-tetrahydrocannabinol (Δ9-THC) for immunoassay application |
| title_full_unstemmed | Development of a plant-produced recombinant monoclonal antibody against Δ-9-tetrahydrocannabinol (Δ9-THC) for immunoassay application |
| title_short | Development of a plant-produced recombinant monoclonal antibody against Δ-9-tetrahydrocannabinol (Δ9-THC) for immunoassay application |
| title_sort | development of a plant produced recombinant monoclonal antibody against δ 9 tetrahydrocannabinol δ9 thc for immunoassay application |
| topic | Plant-produced mAb Transient expression (-)-Δ 9-tetrahydrocannabinol (Δ9-THC) Nicotiana benthamiana, Cannabis extract Immunoassay |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X2200025X |
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