Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer
<p>Abstract</p> <p>Hsulf-1 is a newly identified enzyme, which has the ability to decrease the growth of hepatocellular, ovarian, and head and neck squamous cell carcinoma cells by interfering with heparin-binding growth factor signaling. Since pancreatic cancers over-express a num...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2005-04-01
|
| Series: | Molecular Cancer |
| Subjects: | |
| Online Access: | http://www.molecular-cancer.com/content/4/1/14 |
| _version_ | 1818262914208890880 |
|---|---|
| author | Giese Nathalia A Kayed Hany Abiatari Ivane Kleeff Jörg Li Junsheng Felix Klaus Giese Thomas Büchler Markus W Friess Helmut |
| author_facet | Giese Nathalia A Kayed Hany Abiatari Ivane Kleeff Jörg Li Junsheng Felix Klaus Giese Thomas Büchler Markus W Friess Helmut |
| author_sort | Giese Nathalia A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Hsulf-1 is a newly identified enzyme, which has the ability to decrease the growth of hepatocellular, ovarian, and head and neck squamous cell carcinoma cells by interfering with heparin-binding growth factor signaling. Since pancreatic cancers over-express a number of heparin-binding growth factors and their receptors, the expression and function of this enzyme in pancreatic cancer was analyzed.</p> <p>Results</p> <p>Pancreatic cancer samples expressed significantly (22.5-fold) increased Hsulf-1 mRNA levels compared to normal controls, and Hsulf-1 mRNA was localized in the cancer cells themselves as well as in peritumoral fibroblasts. 4 out of 8 examined pancreatic cancer cell lines expressed Hsulf-1, whereas its expression was below the level of detection in the other cell lines. Stable transfection of the Hsulf-1 negative Panc-1 pancreatic cancer cell line with a full length Hsulf-1 expression vector resulted in increased sulfatase activity and decreased cell-surface heparan-sulfate proteoglycan (HSPG) sulfation. Hsulf-1 expression reduced both anchorage-dependent and -independent cell growth and decreased FGF-2 mediated cell growth and invasion in this cell line.</p> <p>Conclusion</p> <p>High expression of Hsulf-1 occurs in the stromal elements as well as in the tumor cells in pancreatic cancer and interferes with heparin-binding growth factor signaling.</p> |
| first_indexed | 2024-12-12T19:10:41Z |
| format | Article |
| id | doaj.art-d1b75ea8d1cf47b89f559fd761bd4b54 |
| institution | Directory Open Access Journal |
| issn | 1476-4598 |
| language | English |
| last_indexed | 2024-12-12T19:10:41Z |
| publishDate | 2005-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Molecular Cancer |
| spelling | doaj.art-d1b75ea8d1cf47b89f559fd761bd4b542022-12-22T00:14:51ZengBMCMolecular Cancer1476-45982005-04-01411410.1186/1476-4598-4-14Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancerGiese Nathalia AKayed HanyAbiatari IvaneKleeff JörgLi JunshengFelix KlausGiese ThomasBüchler Markus WFriess Helmut<p>Abstract</p> <p>Hsulf-1 is a newly identified enzyme, which has the ability to decrease the growth of hepatocellular, ovarian, and head and neck squamous cell carcinoma cells by interfering with heparin-binding growth factor signaling. Since pancreatic cancers over-express a number of heparin-binding growth factors and their receptors, the expression and function of this enzyme in pancreatic cancer was analyzed.</p> <p>Results</p> <p>Pancreatic cancer samples expressed significantly (22.5-fold) increased Hsulf-1 mRNA levels compared to normal controls, and Hsulf-1 mRNA was localized in the cancer cells themselves as well as in peritumoral fibroblasts. 4 out of 8 examined pancreatic cancer cell lines expressed Hsulf-1, whereas its expression was below the level of detection in the other cell lines. Stable transfection of the Hsulf-1 negative Panc-1 pancreatic cancer cell line with a full length Hsulf-1 expression vector resulted in increased sulfatase activity and decreased cell-surface heparan-sulfate proteoglycan (HSPG) sulfation. Hsulf-1 expression reduced both anchorage-dependent and -independent cell growth and decreased FGF-2 mediated cell growth and invasion in this cell line.</p> <p>Conclusion</p> <p>High expression of Hsulf-1 occurs in the stromal elements as well as in the tumor cells in pancreatic cancer and interferes with heparin-binding growth factor signaling.</p>http://www.molecular-cancer.com/content/4/1/14pancreatic cancergrowth factorssulfataseproteoglycans |
| spellingShingle | Giese Nathalia A Kayed Hany Abiatari Ivane Kleeff Jörg Li Junsheng Felix Klaus Giese Thomas Büchler Markus W Friess Helmut Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer Molecular Cancer pancreatic cancer growth factors sulfatase proteoglycans |
| title | Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer |
| title_full | Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer |
| title_fullStr | Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer |
| title_full_unstemmed | Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer |
| title_short | Enhanced levels of Hsulf-1 interfere with heparin-binding growth factor signaling in pancreatic cancer |
| title_sort | enhanced levels of hsulf 1 interfere with heparin binding growth factor signaling in pancreatic cancer |
| topic | pancreatic cancer growth factors sulfatase proteoglycans |
| url | http://www.molecular-cancer.com/content/4/1/14 |
| work_keys_str_mv | AT giesenathaliaa enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer AT kayedhany enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer AT abiatariivane enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer AT kleeffjorg enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer AT lijunsheng enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer AT felixklaus enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer AT giesethomas enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer AT buchlermarkusw enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer AT friesshelmut enhancedlevelsofhsulf1interferewithheparinbindinggrowthfactorsignalinginpancreaticcancer |