Photo-Inactivation of <i>Staphylococcus aureus</i> by Diaryl-Porphyrins
Photodynamic Antimicrobial Chemotherapy (PACT) has received great attention in recent years since it is an effective and promising modality for the treatment of human oral and skin infections with the advantage of bypassing pathogens’ resistance to antimicrobials. Moreover, PACT applications demonst...
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| Format: | Article |
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MDPI AG
2023-01-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/2/228 |
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| author | Viviana Teresa Orlandi Eleonora Martegani Nicola Trivellin Fabrizio Bolognese Enrico Caruso |
| author_facet | Viviana Teresa Orlandi Eleonora Martegani Nicola Trivellin Fabrizio Bolognese Enrico Caruso |
| author_sort | Viviana Teresa Orlandi |
| collection | DOAJ |
| description | Photodynamic Antimicrobial Chemotherapy (PACT) has received great attention in recent years since it is an effective and promising modality for the treatment of human oral and skin infections with the advantage of bypassing pathogens’ resistance to antimicrobials. Moreover, PACT applications demonstrated a certain activity in the inhibition and eradication of biofilms, overcoming the well-known tolerance of sessile communities to antimicrobial agents. In this study, 13 diaryl-porphyrins (mono-, di-cationic, and non-ionic) <b>P1</b>–<b>P13</b> were investigated for their potential as photosensitizer anti-<i>Staphylococcus aureus</i>. The efficacy of the diaryl-porphyrins was evaluated through photo-inactivation tests. Crystal-violet staining combined with viable count techniques were aimed at assaying their anti-biofilm activity. Among the tested compounds, the neutral photosensitizer <b>P4</b> was better than the cationic ones, irrespective of their corresponding binding rates. In particular, <b>P4</b> was active in inhibiting the biofilm formation and in impairing the viability of the adherent and planktonic populations of a 24 h old biofilm. The inhibitory activity was also efficient against a methicillin resistant <i>S. aureus</i> strain. In conclusion, the diaryl-porphyrin family represents a reservoir of promising compounds for photodynamic applications against the pathogen <i>S. aureus</i> and in preventing the formation of biofilms that cause many infections to become chronic. |
| first_indexed | 2024-03-11T09:15:23Z |
| format | Article |
| id | doaj.art-d1b79f3d279041a9a4fe097ed82efad9 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T09:15:23Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-d1b79f3d279041a9a4fe097ed82efad92023-11-16T18:42:00ZengMDPI AGAntibiotics2079-63822023-01-0112222810.3390/antibiotics12020228Photo-Inactivation of <i>Staphylococcus aureus</i> by Diaryl-PorphyrinsViviana Teresa Orlandi0Eleonora Martegani1Nicola Trivellin2Fabrizio Bolognese3Enrico Caruso4Department of Biotechnologies and Life Sciences, University of Insubria, Via J.H. Dunant 3, 21100 Varese, ItalyDepartment of Biotechnologies and Life Sciences, University of Insubria, Via J.H. Dunant 3, 21100 Varese, ItalyDepartment of Industrial Engineering, University of Padova, Via Gradenigo 6A, 35131 Padova, ItalyDepartment of Biotechnologies and Life Sciences, University of Insubria, Via J.H. Dunant 3, 21100 Varese, ItalyDepartment of Biotechnologies and Life Sciences, University of Insubria, Via J.H. Dunant 3, 21100 Varese, ItalyPhotodynamic Antimicrobial Chemotherapy (PACT) has received great attention in recent years since it is an effective and promising modality for the treatment of human oral and skin infections with the advantage of bypassing pathogens’ resistance to antimicrobials. Moreover, PACT applications demonstrated a certain activity in the inhibition and eradication of biofilms, overcoming the well-known tolerance of sessile communities to antimicrobial agents. In this study, 13 diaryl-porphyrins (mono-, di-cationic, and non-ionic) <b>P1</b>–<b>P13</b> were investigated for their potential as photosensitizer anti-<i>Staphylococcus aureus</i>. The efficacy of the diaryl-porphyrins was evaluated through photo-inactivation tests. Crystal-violet staining combined with viable count techniques were aimed at assaying their anti-biofilm activity. Among the tested compounds, the neutral photosensitizer <b>P4</b> was better than the cationic ones, irrespective of their corresponding binding rates. In particular, <b>P4</b> was active in inhibiting the biofilm formation and in impairing the viability of the adherent and planktonic populations of a 24 h old biofilm. The inhibitory activity was also efficient against a methicillin resistant <i>S. aureus</i> strain. In conclusion, the diaryl-porphyrin family represents a reservoir of promising compounds for photodynamic applications against the pathogen <i>S. aureus</i> and in preventing the formation of biofilms that cause many infections to become chronic.https://www.mdpi.com/2079-6382/12/2/228diaryl-porphyrinsPhotodynamic Antimicrobial Chemotherapy (PACT)<i>Staphylococcus aureus</i> |
| spellingShingle | Viviana Teresa Orlandi Eleonora Martegani Nicola Trivellin Fabrizio Bolognese Enrico Caruso Photo-Inactivation of <i>Staphylococcus aureus</i> by Diaryl-Porphyrins Antibiotics diaryl-porphyrins Photodynamic Antimicrobial Chemotherapy (PACT) <i>Staphylococcus aureus</i> |
| title | Photo-Inactivation of <i>Staphylococcus aureus</i> by Diaryl-Porphyrins |
| title_full | Photo-Inactivation of <i>Staphylococcus aureus</i> by Diaryl-Porphyrins |
| title_fullStr | Photo-Inactivation of <i>Staphylococcus aureus</i> by Diaryl-Porphyrins |
| title_full_unstemmed | Photo-Inactivation of <i>Staphylococcus aureus</i> by Diaryl-Porphyrins |
| title_short | Photo-Inactivation of <i>Staphylococcus aureus</i> by Diaryl-Porphyrins |
| title_sort | photo inactivation of i staphylococcus aureus i by diaryl porphyrins |
| topic | diaryl-porphyrins Photodynamic Antimicrobial Chemotherapy (PACT) <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/2079-6382/12/2/228 |
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