Alteration of the aortic vascular reactivity associated to excessive consumption of Hibiscus sabdariffa Linnaeus: Preliminary findings
The moderate production of reactive oxidative species (ROS) is important because ROS act as second messengers. However, their depletion through the over-activity of the antioxidant system may lead to reductive stress (RS) which is characterized by an increase in reducing equivalents and an elevation...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-09-01
|
| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023072286 |
| _version_ | 1797669797707644928 |
|---|---|
| author | Linaloe Manzano-Pech Verónica Guarner-Lans María Elena Soto Eulises Díaz-Díaz Israel Pérez-Torres |
| author_facet | Linaloe Manzano-Pech Verónica Guarner-Lans María Elena Soto Eulises Díaz-Díaz Israel Pérez-Torres |
| author_sort | Linaloe Manzano-Pech |
| collection | DOAJ |
| description | The moderate production of reactive oxidative species (ROS) is important because ROS act as second messengers. However, their depletion through the over-activity of the antioxidant system may lead to reductive stress (RS) which is characterized by an increase in reducing equivalents and an elevation of some components of the antioxidant system disturbing redox homeostasis. Hibiscus sabdariffa Linnaeus (HSL) is a plant with antioxidant properties that provides compounds that favor the antioxidant system. However, excess chronic consumption could lead to the over expression of the antioxidant enzymatic system, and this could contribute to decrease ROS. Therefore, the objective of this study was to evaluate the alteration of the vascular reactivity associated to excessive and chronic consumption of HSL infusions at different percentages. 40 male Wistar rats were divided into 4 groups. Group 1 control (drinking tap water), group 2, 3 and 4, drinking water supplemented with 15, 30 and 60 g/L of HSL calyxes respectively. The systolic blood pressure (SBP), vascular reactivity, morphological changes, and different components of the enzymatic antioxidant system were evaluated in the thoracic aorta by spectrophotometry. We also determined glucose-6-phosphate dehydrogenase (G6PD), glutathione-S-transferase (GST), thioredoxin-reductase (TrxR), glutathione peroxidase (GPx) and glutathione reductase (GR) and some markers of the non-enzimatic system such as the NO3−/NO2− ratio, glutathione (GSH), selenium, thiols, lipoperoxidation (LPO), and 3-nitrityrosine (3-NT). Vasoconstriction was increased and vasorelaxation was decreased. These alterations were reversed by O2− and H2O2. There was an increase in the wall thickness and elastic fibers (p = 0.004 and p = 0.02, respectively) and in G6PD, GPX, TrxR (p = 0.02, p = 0.03, and p = 0.01 respectively). LPO, GSH (p = 0.01), and selenium (p = 0.04) were decreased. There was a decrease in thiols (p < 0.001), 3-NT (p = 0.04) and GST (p = 0.0005) in rats that received the infusion at 3 and 6%. The excess antioxidants provided by the HSL infusions at 3% and 6% modified vascular reactivity, increasing the enzymatic antioxidant system, and depleting ROS. |
| first_indexed | 2024-03-11T20:49:59Z |
| format | Article |
| id | doaj.art-d1ba0ad3f84f44d7ae5b7bf3d603e914 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-11T20:49:59Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d1ba0ad3f84f44d7ae5b7bf3d603e9142023-10-01T06:02:17ZengElsevierHeliyon2405-84402023-09-0199e20020Alteration of the aortic vascular reactivity associated to excessive consumption of Hibiscus sabdariffa Linnaeus: Preliminary findingsLinaloe Manzano-Pech0Verónica Guarner-Lans1María Elena Soto2Eulises Díaz-Díaz3Israel Pérez-Torres4Departments of Cardiovascular Biomedicine, Institute National of Cardiology Ignacio Chávez, Juan Badiano 1, Section XVI, Tlalpan, 14080, México, USAPhysiology Institute National of Cardiology Ignacio Chávez, Juan Badiano 1, Section XVI, Tlalpan 14080, México, USAImmunology, Institute National of Cardiology Ignacio Chávez, Juan Badiano 1, Section XVI, Tlalpan, 14080, México, USADepartment of Reproductive Biology, Institute National of medical Sciences and Nutrition Salvador Zubirán, Vasco de Quiroga 15, Section XVI, Tlalpan, 14000, México, USADepartments of Cardiovascular Biomedicine, Institute National of Cardiology Ignacio Chávez, Juan Badiano 1, Section XVI, Tlalpan, 14080, México, USA; Corresponding author.The moderate production of reactive oxidative species (ROS) is important because ROS act as second messengers. However, their depletion through the over-activity of the antioxidant system may lead to reductive stress (RS) which is characterized by an increase in reducing equivalents and an elevation of some components of the antioxidant system disturbing redox homeostasis. Hibiscus sabdariffa Linnaeus (HSL) is a plant with antioxidant properties that provides compounds that favor the antioxidant system. However, excess chronic consumption could lead to the over expression of the antioxidant enzymatic system, and this could contribute to decrease ROS. Therefore, the objective of this study was to evaluate the alteration of the vascular reactivity associated to excessive and chronic consumption of HSL infusions at different percentages. 40 male Wistar rats were divided into 4 groups. Group 1 control (drinking tap water), group 2, 3 and 4, drinking water supplemented with 15, 30 and 60 g/L of HSL calyxes respectively. The systolic blood pressure (SBP), vascular reactivity, morphological changes, and different components of the enzymatic antioxidant system were evaluated in the thoracic aorta by spectrophotometry. We also determined glucose-6-phosphate dehydrogenase (G6PD), glutathione-S-transferase (GST), thioredoxin-reductase (TrxR), glutathione peroxidase (GPx) and glutathione reductase (GR) and some markers of the non-enzimatic system such as the NO3−/NO2− ratio, glutathione (GSH), selenium, thiols, lipoperoxidation (LPO), and 3-nitrityrosine (3-NT). Vasoconstriction was increased and vasorelaxation was decreased. These alterations were reversed by O2− and H2O2. There was an increase in the wall thickness and elastic fibers (p = 0.004 and p = 0.02, respectively) and in G6PD, GPX, TrxR (p = 0.02, p = 0.03, and p = 0.01 respectively). LPO, GSH (p = 0.01), and selenium (p = 0.04) were decreased. There was a decrease in thiols (p < 0.001), 3-NT (p = 0.04) and GST (p = 0.0005) in rats that received the infusion at 3 and 6%. The excess antioxidants provided by the HSL infusions at 3% and 6% modified vascular reactivity, increasing the enzymatic antioxidant system, and depleting ROS.http://www.sciencedirect.com/science/article/pii/S2405844023072286HypertensionVascular functionAortaReductive stressHSLAntioxidant system |
| spellingShingle | Linaloe Manzano-Pech Verónica Guarner-Lans María Elena Soto Eulises Díaz-Díaz Israel Pérez-Torres Alteration of the aortic vascular reactivity associated to excessive consumption of Hibiscus sabdariffa Linnaeus: Preliminary findings Heliyon Hypertension Vascular function Aorta Reductive stress HSL Antioxidant system |
| title | Alteration of the aortic vascular reactivity associated to excessive consumption of Hibiscus sabdariffa Linnaeus: Preliminary findings |
| title_full | Alteration of the aortic vascular reactivity associated to excessive consumption of Hibiscus sabdariffa Linnaeus: Preliminary findings |
| title_fullStr | Alteration of the aortic vascular reactivity associated to excessive consumption of Hibiscus sabdariffa Linnaeus: Preliminary findings |
| title_full_unstemmed | Alteration of the aortic vascular reactivity associated to excessive consumption of Hibiscus sabdariffa Linnaeus: Preliminary findings |
| title_short | Alteration of the aortic vascular reactivity associated to excessive consumption of Hibiscus sabdariffa Linnaeus: Preliminary findings |
| title_sort | alteration of the aortic vascular reactivity associated to excessive consumption of hibiscus sabdariffa linnaeus preliminary findings |
| topic | Hypertension Vascular function Aorta Reductive stress HSL Antioxidant system |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023072286 |
| work_keys_str_mv | AT linaloemanzanopech alterationoftheaorticvascularreactivityassociatedtoexcessiveconsumptionofhibiscussabdariffalinnaeuspreliminaryfindings AT veronicaguarnerlans alterationoftheaorticvascularreactivityassociatedtoexcessiveconsumptionofhibiscussabdariffalinnaeuspreliminaryfindings AT mariaelenasoto alterationoftheaorticvascularreactivityassociatedtoexcessiveconsumptionofhibiscussabdariffalinnaeuspreliminaryfindings AT eulisesdiazdiaz alterationoftheaorticvascularreactivityassociatedtoexcessiveconsumptionofhibiscussabdariffalinnaeuspreliminaryfindings AT israelpereztorres alterationoftheaorticvascularreactivityassociatedtoexcessiveconsumptionofhibiscussabdariffalinnaeuspreliminaryfindings |