Effect of selenium nanoparticles on the quality and fertility of short-term preserved boar semen
This study was carried out to investigate the effect of different concentrations of selenium nanoparticles (Se-NPs) in the Beltsville Thawing Solution (BTS) extender on the semen quality and fertility of Hampshire crossbred pigs. For the study, semen was collected from four boars (10 ejaculates/boar...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2023.1333841/full |
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| author | Dipan Rudra Paul Dibyajyoti Talukdar Fazal Ali Ahmed K. Lalrintluanga Girin Kalita T. C. Tolenkhomba Himsikha Chakravarty Rahul Katiyar Gautam Khargharia Sourabh Deori |
| author_facet | Dipan Rudra Paul Dibyajyoti Talukdar Fazal Ali Ahmed K. Lalrintluanga Girin Kalita T. C. Tolenkhomba Himsikha Chakravarty Rahul Katiyar Gautam Khargharia Sourabh Deori |
| author_sort | Dipan Rudra Paul |
| collection | DOAJ |
| description | This study was carried out to investigate the effect of different concentrations of selenium nanoparticles (Se-NPs) in the Beltsville Thawing Solution (BTS) extender on the semen quality and fertility of Hampshire crossbred pigs. For the study, semen was collected from four boars (10 ejaculates/boar) by the gloved hand method. Each ejaculate was extended @ 1:2 with the BTS extender and split into four aliquots. The control (C) samples were without the supplementation of Se-NPs, whereas the other three were supplemented with 0.5 (T1), 1 (T2), and 2 μg ml−1 of Se-NPs (T3) and stored at 15°C in a BOD incubator. Extended semen was evaluated at 0 (immediately after dilution), 24, 48, 72, and 96 h of storage for sperm motility, live sperm, plasma membrane integrity, acrosome integrity, DNA integrity, and mitochondrial membrane potential (MMP). The mean percentage of sperm motility, live sperm, and sperm with intact plasma membrane and acrosome, and MMPs were significantly (p < 0.01) higher in all treated groups in comparison to control at 24, 48, 72, and 96 h of storage. Sperm with intact DNA in all treated groups increased significantly at 48 (p < 0.05), and 72 and 96 (p < 0.01) h of storage in comparison to the control group. The concentration of 1 μg ml−1 of Se-NPs was found to be the best among other concentrations. In each group, 10 sows were artificially inseminated with the liquid semen preserved for 72 h at 15°C. Supplementation of 1 μg ml−1 of Se-NPs yielded the highest conception rate in comparison to other groups. In conclusion, supplementation of 1 μg ml−1 of Se-NPs in the BTS extender resulted in the best semen quality and conception rate during the short-time liquid preservation of boar semen. |
| first_indexed | 2024-03-08T12:08:06Z |
| format | Article |
| id | doaj.art-d1bab0d5a6934129a1417a3814117a03 |
| institution | Directory Open Access Journal |
| issn | 2297-1769 |
| language | English |
| last_indexed | 2024-03-08T12:08:06Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj.art-d1bab0d5a6934129a1417a3814117a032024-01-23T04:24:48ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-01-011010.3389/fvets.2023.13338411333841Effect of selenium nanoparticles on the quality and fertility of short-term preserved boar semenDipan Rudra Paul0Dibyajyoti Talukdar1Fazal Ali Ahmed2K. Lalrintluanga3Girin Kalita4T. C. Tolenkhomba5Himsikha Chakravarty6Rahul Katiyar7Gautam Khargharia8Sourabh Deori9College of Veterinary Sciences & A.H, Central Agricultural University, Aizawl, Mizoram, IndiaCollege of Veterinary Sciences & A.H, Central Agricultural University, Aizawl, Mizoram, IndiaCollege of Veterinary Sciences & A.H, Central Agricultural University, Aizawl, Mizoram, IndiaCollege of Veterinary Sciences & A.H, Central Agricultural University, Aizawl, Mizoram, IndiaCollege of Veterinary Sciences & A.H, Central Agricultural University, Aizawl, Mizoram, IndiaCollege of Veterinary Sciences & A.H, Central Agricultural University, Aizawl, Mizoram, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, Meghalaya, IndiaDivision of Animal and Fisheries Sciences, ICAR Research Complex for NEH Region, Umiam, Meghalaya, IndiaThis study was carried out to investigate the effect of different concentrations of selenium nanoparticles (Se-NPs) in the Beltsville Thawing Solution (BTS) extender on the semen quality and fertility of Hampshire crossbred pigs. For the study, semen was collected from four boars (10 ejaculates/boar) by the gloved hand method. Each ejaculate was extended @ 1:2 with the BTS extender and split into four aliquots. The control (C) samples were without the supplementation of Se-NPs, whereas the other three were supplemented with 0.5 (T1), 1 (T2), and 2 μg ml−1 of Se-NPs (T3) and stored at 15°C in a BOD incubator. Extended semen was evaluated at 0 (immediately after dilution), 24, 48, 72, and 96 h of storage for sperm motility, live sperm, plasma membrane integrity, acrosome integrity, DNA integrity, and mitochondrial membrane potential (MMP). The mean percentage of sperm motility, live sperm, and sperm with intact plasma membrane and acrosome, and MMPs were significantly (p < 0.01) higher in all treated groups in comparison to control at 24, 48, 72, and 96 h of storage. Sperm with intact DNA in all treated groups increased significantly at 48 (p < 0.05), and 72 and 96 (p < 0.01) h of storage in comparison to the control group. The concentration of 1 μg ml−1 of Se-NPs was found to be the best among other concentrations. In each group, 10 sows were artificially inseminated with the liquid semen preserved for 72 h at 15°C. Supplementation of 1 μg ml−1 of Se-NPs yielded the highest conception rate in comparison to other groups. In conclusion, supplementation of 1 μg ml−1 of Se-NPs in the BTS extender resulted in the best semen quality and conception rate during the short-time liquid preservation of boar semen.https://www.frontiersin.org/articles/10.3389/fvets.2023.1333841/fullselenium nanoparticlesboar semenBTS extenderliquid preservationantioxidants |
| spellingShingle | Dipan Rudra Paul Dibyajyoti Talukdar Fazal Ali Ahmed K. Lalrintluanga Girin Kalita T. C. Tolenkhomba Himsikha Chakravarty Rahul Katiyar Gautam Khargharia Sourabh Deori Effect of selenium nanoparticles on the quality and fertility of short-term preserved boar semen Frontiers in Veterinary Science selenium nanoparticles boar semen BTS extender liquid preservation antioxidants |
| title | Effect of selenium nanoparticles on the quality and fertility of short-term preserved boar semen |
| title_full | Effect of selenium nanoparticles on the quality and fertility of short-term preserved boar semen |
| title_fullStr | Effect of selenium nanoparticles on the quality and fertility of short-term preserved boar semen |
| title_full_unstemmed | Effect of selenium nanoparticles on the quality and fertility of short-term preserved boar semen |
| title_short | Effect of selenium nanoparticles on the quality and fertility of short-term preserved boar semen |
| title_sort | effect of selenium nanoparticles on the quality and fertility of short term preserved boar semen |
| topic | selenium nanoparticles boar semen BTS extender liquid preservation antioxidants |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2023.1333841/full |
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