Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes
Serial block-face scanning electron microscopy (SBF-SEM) was used here to study tobacco male meiosis. Three-dimensional ultrastructural analyses revealed that intercellular nuclear migration (INM) occurs in 90–100% of tobacco meiocytes. At the very beginning of meiosis, every meiocyte connected with...
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Frontiers Media S.A.
2021-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2021.672642/full |
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| author | Sergey Mursalimov Nobuhiko Ohno Nobuhiko Ohno Mami Matsumoto Sergey Bayborodin Elena Deineko |
| author_facet | Sergey Mursalimov Nobuhiko Ohno Nobuhiko Ohno Mami Matsumoto Sergey Bayborodin Elena Deineko |
| author_sort | Sergey Mursalimov |
| collection | DOAJ |
| description | Serial block-face scanning electron microscopy (SBF-SEM) was used here to study tobacco male meiosis. Three-dimensional ultrastructural analyses revealed that intercellular nuclear migration (INM) occurs in 90–100% of tobacco meiocytes. At the very beginning of meiosis, every meiocyte connected with neighboring cells by more than 100 channels was capable of INM. At leptotene and zygotene, the nucleus in most tobacco meiocytes approached the cell wall and formed nuclear protuberances (NPs) that crossed the cell wall through the channels and extended into the cytoplasm of a neighboring cell. The separation of NPs from the migrating nuclei and micronuclei formation were not observed. In some cases, the NPs and nuclei of neighboring cells appeared apposed to each other, and the gap between their nuclear membranes became invisible. At pachytene, NPs retracted into their own cells. After that, the INM stopped. We consider INM a normal part of tobacco meiosis, but the reason for such behavior of nuclei is unclear. The results obtained by SBF-SEM suggest that there are still many unexplored features of plant meiosis hidden by limitations of common types of microscopy and that SBF-SEM can turn over a new leaf in plant meiosis research. |
| first_indexed | 2024-04-11T18:13:14Z |
| format | Article |
| id | doaj.art-d1bee1cfbf324b11831acaefe3dca47f |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-04-11T18:13:14Z |
| publishDate | 2021-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-d1bee1cfbf324b11831acaefe3dca47f2022-12-22T04:10:03ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2021-05-011210.3389/fpls.2021.672642672642Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male MeiocytesSergey Mursalimov0Nobuhiko Ohno1Nobuhiko Ohno2Mami Matsumoto3Sergey Bayborodin4Elena Deineko5Laboratory of Plant Bioengineering, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaDepartment of Anatomy, Division of Histology and Cell Biology, School of Medicine, Jichi Medical University, Shimotsuke, JapanDivision of Ultrastructural Research, National Institute for Physiological Sciences, Okazaki, JapanSection of Electron Microscopy, Supportive Center for Brain Research, National Institute for Physiological Sciences, Okazaki, JapanLaboratory of Plant Bioengineering, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaLaboratory of Plant Bioengineering, Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, RussiaSerial block-face scanning electron microscopy (SBF-SEM) was used here to study tobacco male meiosis. Three-dimensional ultrastructural analyses revealed that intercellular nuclear migration (INM) occurs in 90–100% of tobacco meiocytes. At the very beginning of meiosis, every meiocyte connected with neighboring cells by more than 100 channels was capable of INM. At leptotene and zygotene, the nucleus in most tobacco meiocytes approached the cell wall and formed nuclear protuberances (NPs) that crossed the cell wall through the channels and extended into the cytoplasm of a neighboring cell. The separation of NPs from the migrating nuclei and micronuclei formation were not observed. In some cases, the NPs and nuclei of neighboring cells appeared apposed to each other, and the gap between their nuclear membranes became invisible. At pachytene, NPs retracted into their own cells. After that, the INM stopped. We consider INM a normal part of tobacco meiosis, but the reason for such behavior of nuclei is unclear. The results obtained by SBF-SEM suggest that there are still many unexplored features of plant meiosis hidden by limitations of common types of microscopy and that SBF-SEM can turn over a new leaf in plant meiosis research.https://www.frontiersin.org/articles/10.3389/fpls.2021.672642/fullvolume electron microscopy (vEM)cytomixismale meiosisNicotianaserial block-face scanning electron microscopynuclear migration |
| spellingShingle | Sergey Mursalimov Nobuhiko Ohno Nobuhiko Ohno Mami Matsumoto Sergey Bayborodin Elena Deineko Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes Frontiers in Plant Science volume electron microscopy (vEM) cytomixis male meiosis Nicotiana serial block-face scanning electron microscopy nuclear migration |
| title | Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes |
| title_full | Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes |
| title_fullStr | Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes |
| title_full_unstemmed | Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes |
| title_short | Serial Block-Face Scanning Electron Microscopy Reveals That Intercellular Nuclear Migration Occurs in Most Normal Tobacco Male Meiocytes |
| title_sort | serial block face scanning electron microscopy reveals that intercellular nuclear migration occurs in most normal tobacco male meiocytes |
| topic | volume electron microscopy (vEM) cytomixis male meiosis Nicotiana serial block-face scanning electron microscopy nuclear migration |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2021.672642/full |
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