Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium Chloride
Abstract Silsesquioxane 3-n-propylpyridinium chloride (SiPy+Cl-) is a water-soluble polymer that can be used as a promising material with remarkable biological effects. SiPy+Cl- can form thin films on substrate surfaces and shows suitable adhesiveness. Besides, it has high affinity for metal ions. C...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2022-10-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100337&tlng=en |
| _version_ | 1798030478586937344 |
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| author | Flávia de Brito Pedroso Deize Basílio dos Santos de Aguiar Priscilla Salles de Brito Christiana Andrade Pessôa Sérgio Toshio Fujiwara Carla Cristine Kanunfre Ruben Auccaise Estrada Sinvaldo Baglie Jessica Mendes Nadal Andressa Novatski Paulo Vitor Farago Alessandro Dourado Loguércio |
| author_facet | Flávia de Brito Pedroso Deize Basílio dos Santos de Aguiar Priscilla Salles de Brito Christiana Andrade Pessôa Sérgio Toshio Fujiwara Carla Cristine Kanunfre Ruben Auccaise Estrada Sinvaldo Baglie Jessica Mendes Nadal Andressa Novatski Paulo Vitor Farago Alessandro Dourado Loguércio |
| author_sort | Flávia de Brito Pedroso |
| collection | DOAJ |
| description | Abstract Silsesquioxane 3-n-propylpyridinium chloride (SiPy+Cl-) is a water-soluble polymer that can be used as a promising material with remarkable biological effects. SiPy+Cl- can form thin films on substrate surfaces and shows suitable adhesiveness. Besides, it has high affinity for metal ions. Considering this effect as exchanger polymer and the well-known antimicrobial and cytotoxic features of copper, the aim of this study was to perform the copper functionalization of silsesquioxane 3-n-propylpyridinium chloride Cu-SiPy+Cl-. The SiPy+Cl- was obtained by the sol-gel processing method and the incorporation of copper (II) chloride was carried out by immobilization. Its characterization was performed by spectroscopic methods of Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). The FTIR spectrum of SiPy+Cl- showed symmetric and asymmetric stretching of Si-O-Si group and also exhibited vibrational bands of pyridinium ring. The FTIR spectrum of Cu-SiPy+Cl- assigned typical bands displacements of the metal coordination to nitrogen atoms. Spectroscopic data recorded by 13C and 29S NMR confirmed the chemical structures of SiPy+Cl- and Cu-SiPy+Cl-. Concerning to the in vitro susceptibility assay, the antimicrobial activity against Staphylococcus aureus, Escherichia coli and Streptococus mutans was achieved by the agar-well diffusion method. Cu-SiPy+Cl- showed similar cytotoxicity than SiPy+Cl- against Calu-3 and 3T3 cell lines by the MTT colorimetric assay. Further studies are required to investigate the use of Cu-SiPy+Cl- as a novel dental material. |
| first_indexed | 2024-04-11T19:42:10Z |
| format | Article |
| id | doaj.art-d1c1f75dfc124eb89603576d97fe6fee |
| institution | Directory Open Access Journal |
| issn | 1678-4324 |
| language | English |
| last_indexed | 2024-04-11T19:42:10Z |
| publishDate | 2022-10-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-d1c1f75dfc124eb89603576d97fe6fee2022-12-22T04:06:41ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1678-43242022-10-016510.1590/1678-4324-2022220228Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium ChlorideFlávia de Brito Pedrosohttps://orcid.org/0000-0002-3533-8867Deize Basílio dos Santos de Aguiarhttps://orcid.org/0000-0002-2059-6871Priscilla Salles de Britohttps://orcid.org/0000-0002-1419-8711Christiana Andrade Pessôahttps://orcid.org/0000-0001-8973-5908Sérgio Toshio Fujiwarahttps://orcid.org/0000-0001-5887-7575Carla Cristine Kanunfrehttps://orcid.org/0000-0002-2865-3084Ruben Auccaise Estradahttps://orcid.org/0000-0002-9602-6533Sinvaldo Bagliehttps://orcid.org/0000-0002-4688-6463Jessica Mendes Nadalhttps://orcid.org/0000-0002-2419-2110Andressa Novatskihttps://orcid.org/0000-0002-8327-6285Paulo Vitor Faragohttps://orcid.org/0000-0002-9934-4027Alessandro Dourado Loguérciohttps://orcid.org/0000-0001-9880-4856Abstract Silsesquioxane 3-n-propylpyridinium chloride (SiPy+Cl-) is a water-soluble polymer that can be used as a promising material with remarkable biological effects. SiPy+Cl- can form thin films on substrate surfaces and shows suitable adhesiveness. Besides, it has high affinity for metal ions. Considering this effect as exchanger polymer and the well-known antimicrobial and cytotoxic features of copper, the aim of this study was to perform the copper functionalization of silsesquioxane 3-n-propylpyridinium chloride Cu-SiPy+Cl-. The SiPy+Cl- was obtained by the sol-gel processing method and the incorporation of copper (II) chloride was carried out by immobilization. Its characterization was performed by spectroscopic methods of Fourier-transform infrared spectroscopy (FTIR) and nuclear magnetic resonance (NMR). The FTIR spectrum of SiPy+Cl- showed symmetric and asymmetric stretching of Si-O-Si group and also exhibited vibrational bands of pyridinium ring. The FTIR spectrum of Cu-SiPy+Cl- assigned typical bands displacements of the metal coordination to nitrogen atoms. Spectroscopic data recorded by 13C and 29S NMR confirmed the chemical structures of SiPy+Cl- and Cu-SiPy+Cl-. Concerning to the in vitro susceptibility assay, the antimicrobial activity against Staphylococcus aureus, Escherichia coli and Streptococus mutans was achieved by the agar-well diffusion method. Cu-SiPy+Cl- showed similar cytotoxicity than SiPy+Cl- against Calu-3 and 3T3 cell lines by the MTT colorimetric assay. Further studies are required to investigate the use of Cu-SiPy+Cl- as a novel dental material.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100337&tlng=enantimicrobial activitycell viabilitycopper functionalizationnovel dental materials. |
| spellingShingle | Flávia de Brito Pedroso Deize Basílio dos Santos de Aguiar Priscilla Salles de Brito Christiana Andrade Pessôa Sérgio Toshio Fujiwara Carla Cristine Kanunfre Ruben Auccaise Estrada Sinvaldo Baglie Jessica Mendes Nadal Andressa Novatski Paulo Vitor Farago Alessandro Dourado Loguércio Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium Chloride Brazilian Archives of Biology and Technology antimicrobial activity cell viability copper functionalization novel dental materials. |
| title | Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium Chloride |
| title_full | Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium Chloride |
| title_fullStr | Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium Chloride |
| title_full_unstemmed | Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium Chloride |
| title_short | Antibacterial and Cytotoxic Activities of Copper-Functionalized Silsesquioxane 3-n-Propylpyridinium Chloride |
| title_sort | antibacterial and cytotoxic activities of copper functionalized silsesquioxane 3 n propylpyridinium chloride |
| topic | antimicrobial activity cell viability copper functionalization novel dental materials. |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132022000100337&tlng=en |
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