Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food
Hydroxylated polychlorinated biphenyls (OH-PCBs) are a group of metabolites biotransformed from polychlorinated biphenyls by animals with higher toxicities than their parent compounds. The present work developed and validated an analytical method for determinating penta-, hexa-, and hepta-chlorine s...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-10-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/27/21/7247 |
| _version_ | 1797467133169369088 |
|---|---|
| author | Wenyu Zhao Na Cui Yuan Huang Zihao Wang Peilong Wang Haijun Liang Ruiguo Wang Xiaoou Su |
| author_facet | Wenyu Zhao Na Cui Yuan Huang Zihao Wang Peilong Wang Haijun Liang Ruiguo Wang Xiaoou Su |
| author_sort | Wenyu Zhao |
| collection | DOAJ |
| description | Hydroxylated polychlorinated biphenyls (OH-PCBs) are a group of metabolites biotransformed from polychlorinated biphenyls by animals with higher toxicities than their parent compounds. The present work developed and validated an analytical method for determinating penta-, hexa-, and hepta-chlorine substituted OH-PCBs in animal-derived food based on ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) with isotope-dilution. The target analytes were extracted with a 50% n-hexane/dichloromethane (<i>v</i>/<i>v</i>), purified by sulfuric acid-silica gel, and separated by 5% hydrated silica gel, achieving a final concentration of 100 times before injection to LC–MS/MS. The limits of detection (LOD) and quantification (LOQ) for target OH-PCBs were within the ranges of 0.003–0.010 μg/kg and 0.009–0.030 μg/kg, respectively. Average recoveries ranged between 76.7% and 116.5%, with relative standard deviations of less than 18.4%. The proposed method is simple, time-saving, sensitive, and accurate, making it a powerful tool for risk monitoring of OH-PCBs in animal-derived food. |
| first_indexed | 2024-03-09T18:49:21Z |
| format | Article |
| id | doaj.art-d1c2fcda37b94bf990db8bad4bdfa343 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:49:21Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-d1c2fcda37b94bf990db8bad4bdfa3432023-11-24T06:01:04ZengMDPI AGMolecules1420-30492022-10-012721724710.3390/molecules27217247Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived FoodWenyu Zhao0Na Cui1Yuan Huang2Zihao Wang3Peilong Wang4Haijun Liang5Ruiguo Wang6Xiaoou Su7Institute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCHINA FEED Magazine Agency, Beijing 100710, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaInstitute of Quality Standards and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaHydroxylated polychlorinated biphenyls (OH-PCBs) are a group of metabolites biotransformed from polychlorinated biphenyls by animals with higher toxicities than their parent compounds. The present work developed and validated an analytical method for determinating penta-, hexa-, and hepta-chlorine substituted OH-PCBs in animal-derived food based on ultra-performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) with isotope-dilution. The target analytes were extracted with a 50% n-hexane/dichloromethane (<i>v</i>/<i>v</i>), purified by sulfuric acid-silica gel, and separated by 5% hydrated silica gel, achieving a final concentration of 100 times before injection to LC–MS/MS. The limits of detection (LOD) and quantification (LOQ) for target OH-PCBs were within the ranges of 0.003–0.010 μg/kg and 0.009–0.030 μg/kg, respectively. Average recoveries ranged between 76.7% and 116.5%, with relative standard deviations of less than 18.4%. The proposed method is simple, time-saving, sensitive, and accurate, making it a powerful tool for risk monitoring of OH-PCBs in animal-derived food.https://www.mdpi.com/1420-3049/27/21/7247hydroxylated polychlorinated biphenylsfooddeterminationmetaboliteUPLC–MS/MS |
| spellingShingle | Wenyu Zhao Na Cui Yuan Huang Zihao Wang Peilong Wang Haijun Liang Ruiguo Wang Xiaoou Su Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food Molecules hydroxylated polychlorinated biphenyls food determination metabolite UPLC–MS/MS |
| title | Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food |
| title_full | Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food |
| title_fullStr | Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food |
| title_full_unstemmed | Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food |
| title_short | Development of an Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry Method for Hydroxylated Polychlorinated Biphenyls in Animal-Derived Food |
| title_sort | development of an ultra performance liquid chromatography tandem mass spectrometry method for hydroxylated polychlorinated biphenyls in animal derived food |
| topic | hydroxylated polychlorinated biphenyls food determination metabolite UPLC–MS/MS |
| url | https://www.mdpi.com/1420-3049/27/21/7247 |
| work_keys_str_mv | AT wenyuzhao developmentofanultraperformanceliquidchromatographytandemmassspectrometrymethodforhydroxylatedpolychlorinatedbiphenylsinanimalderivedfood AT nacui developmentofanultraperformanceliquidchromatographytandemmassspectrometrymethodforhydroxylatedpolychlorinatedbiphenylsinanimalderivedfood AT yuanhuang developmentofanultraperformanceliquidchromatographytandemmassspectrometrymethodforhydroxylatedpolychlorinatedbiphenylsinanimalderivedfood AT zihaowang developmentofanultraperformanceliquidchromatographytandemmassspectrometrymethodforhydroxylatedpolychlorinatedbiphenylsinanimalderivedfood AT peilongwang developmentofanultraperformanceliquidchromatographytandemmassspectrometrymethodforhydroxylatedpolychlorinatedbiphenylsinanimalderivedfood AT haijunliang developmentofanultraperformanceliquidchromatographytandemmassspectrometrymethodforhydroxylatedpolychlorinatedbiphenylsinanimalderivedfood AT ruiguowang developmentofanultraperformanceliquidchromatographytandemmassspectrometrymethodforhydroxylatedpolychlorinatedbiphenylsinanimalderivedfood AT xiaoousu developmentofanultraperformanceliquidchromatographytandemmassspectrometrymethodforhydroxylatedpolychlorinatedbiphenylsinanimalderivedfood |