Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs.
In order to monitor the occurrence of zoonotic agents in pig herds as well as to improve herd health management, the development of new cost-effective diagnostic methods for pigs is necessary. In this study, a protein microarray-based assay for the simultaneous detection of immunoglobulin G (IgG) an...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2019-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0217290 |
| _version_ | 1797661411825942528 |
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| author | Katharina Loreck Sylvia Mitrenga Diana Meemken Regina Heinze Annett Reissig Elke Mueller Ralf Ehricht Claudia Engemann Matthias Greiner |
| author_facet | Katharina Loreck Sylvia Mitrenga Diana Meemken Regina Heinze Annett Reissig Elke Mueller Ralf Ehricht Claudia Engemann Matthias Greiner |
| author_sort | Katharina Loreck |
| collection | DOAJ |
| description | In order to monitor the occurrence of zoonotic agents in pig herds as well as to improve herd health management, the development of new cost-effective diagnostic methods for pigs is necessary. In this study, a protein microarray-based assay for the simultaneous detection of immunoglobulin G (IgG) antibodies against different zoonotic agents and pathogens causing production diseases in pigs was developed. Therefore, antigens of ten different important swine pathogens (Toxoplasma gondii, Yersinia enterocolitica, Salmonella spp., Trichinella spp., Mycobacterium avium, Hepatitis E virus, Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, the porcine reproductive and respiratory syndrome virus, Influenza A virus) were spotted and covalently immobilized as 'antigen-spots' on microarray chips in order to test pig serum for the occurrence of antibodies. Pig serum was sampled at three German abattoirs and ELISA tests for the different pathogens were conducted with the purpose of creating a panel of reference samples for microarray analysis. To evaluate the accuracy of the antigens on the microarray, receiver operating characteristic (ROC) curve analysis using the ELISA test results as reference was performed for the different antigens. High area under curve values were achieved for the antigens of two zoonotic agents: Toxoplasma gondii (0.91), Yersinia enterocolitica (0.97) and for three production diseases: Actinobacillus pleuropneumoniae (0.77), Mycoplasma hyopneumoniae (0.94) and the porcine reproductive and respiratory syndrome virus (0.87). With the help of the newly developed microarray assay, collecting data on the occurrence of antibodies against zoonotic agents and production diseases in pig herds could be minimized to one measurement, resulting in an efficient screening test. |
| first_indexed | 2024-03-11T18:45:06Z |
| format | Article |
| id | doaj.art-d1c30f64dec748bcb7141cf0da98b846 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-11T18:45:06Z |
| publishDate | 2019-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-d1c30f64dec748bcb7141cf0da98b8462023-10-12T05:31:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032019-01-01145e021729010.1371/journal.pone.0217290Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs.Katharina LoreckSylvia MitrengaDiana MeemkenRegina HeinzeAnnett ReissigElke MuellerRalf EhrichtClaudia EngemannMatthias GreinerIn order to monitor the occurrence of zoonotic agents in pig herds as well as to improve herd health management, the development of new cost-effective diagnostic methods for pigs is necessary. In this study, a protein microarray-based assay for the simultaneous detection of immunoglobulin G (IgG) antibodies against different zoonotic agents and pathogens causing production diseases in pigs was developed. Therefore, antigens of ten different important swine pathogens (Toxoplasma gondii, Yersinia enterocolitica, Salmonella spp., Trichinella spp., Mycobacterium avium, Hepatitis E virus, Mycoplasma hyopneumoniae, Actinobacillus pleuropneumoniae, the porcine reproductive and respiratory syndrome virus, Influenza A virus) were spotted and covalently immobilized as 'antigen-spots' on microarray chips in order to test pig serum for the occurrence of antibodies. Pig serum was sampled at three German abattoirs and ELISA tests for the different pathogens were conducted with the purpose of creating a panel of reference samples for microarray analysis. To evaluate the accuracy of the antigens on the microarray, receiver operating characteristic (ROC) curve analysis using the ELISA test results as reference was performed for the different antigens. High area under curve values were achieved for the antigens of two zoonotic agents: Toxoplasma gondii (0.91), Yersinia enterocolitica (0.97) and for three production diseases: Actinobacillus pleuropneumoniae (0.77), Mycoplasma hyopneumoniae (0.94) and the porcine reproductive and respiratory syndrome virus (0.87). With the help of the newly developed microarray assay, collecting data on the occurrence of antibodies against zoonotic agents and production diseases in pig herds could be minimized to one measurement, resulting in an efficient screening test.https://doi.org/10.1371/journal.pone.0217290 |
| spellingShingle | Katharina Loreck Sylvia Mitrenga Diana Meemken Regina Heinze Annett Reissig Elke Mueller Ralf Ehricht Claudia Engemann Matthias Greiner Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. PLoS ONE |
| title | Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. |
| title_full | Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. |
| title_fullStr | Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. |
| title_full_unstemmed | Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. |
| title_short | Development of a miniaturized protein microarray as a new serological IgG screening test for zoonotic agents and production diseases in pigs. |
| title_sort | development of a miniaturized protein microarray as a new serological igg screening test for zoonotic agents and production diseases in pigs |
| url | https://doi.org/10.1371/journal.pone.0217290 |
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