HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan <i>Anacyclus clavatus</i>
In this work, polyphenols were extracted from <i>Anacylus clavatus</i> flowers using a hydroethanolic solvent, and the obtained extract was studied for its total phenol and flavonoid contents and evaluated for its antioxidant and antibacterial capacities. The contents of total phenols an...
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MDPI AG
2022-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/24/3423 |
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| author | Mounia Chroho Mustapha Aazza Aziz Bouymajane Yassine Oulad El Majdoub Francesco Cacciola Luigi Mondello Touriya Zair Latifa Bouissane |
| author_facet | Mounia Chroho Mustapha Aazza Aziz Bouymajane Yassine Oulad El Majdoub Francesco Cacciola Luigi Mondello Touriya Zair Latifa Bouissane |
| author_sort | Mounia Chroho |
| collection | DOAJ |
| description | In this work, polyphenols were extracted from <i>Anacylus clavatus</i> flowers using a hydroethanolic solvent, and the obtained extract was studied for its total phenol and flavonoid contents and evaluated for its antioxidant and antibacterial capacities. The contents of total phenols and flavonoids were measured by employing gallic acid and quercetin as references, respectively, and the phenolic composition analysis was conducted using high-performance liquid chromatography combined with a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA/ESI-MS). The antioxidant capacity of the extracts was tested using a potassium ferric reducing antioxidant power (PFRAP) assay, and the antibacterial activity assay was carried out against Gram-negative bacteria (<i>Escherichia coli</i> and <i>Salmonella typhimirium</i>) and Gram-positive bacteria (<i>Staphyloccocus aureus</i> and <i>Listeria monocytogenes</i>) using the broth microdilution assay. The phenolic and flavonoid contents of the extracts equaled 9.53 ± 0.48 mg GAE/g dm and 1.31 ± 0.06 mg QE/g dm, respectively. The chromatographic analysis of the phenolic profile detected 26 phenolic compounds belonging to phenolic acids, flavones and flavonols, and with the caffeoylquinic acid derivatives being the major phenolic compounds present in 12 isomers. Only one organic compound, <i>viz.</i> citric acid, was found. The extracts exhibited interesting antioxidant activity. Bacteriostatic activity towards <i>Escherichia coli</i> and bactericidal activity against <i>Salmonella typhimirium</i>, <i>Staphyloccocus aureus</i> and <i>Listeria monocytogenes</i> were determined. This study revealed that <i>Anacyclus clavatus</i> flower extracts contain phenolic compounds with interesting bioactivities. |
| first_indexed | 2024-03-09T15:57:47Z |
| format | Article |
| id | doaj.art-d1cecdf92dda467eac6551e9922cdbeb |
| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-09T15:57:47Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-d1cecdf92dda467eac6551e9922cdbeb2023-11-24T17:27:00ZengMDPI AGPlants2223-77472022-12-011124342310.3390/plants11243423HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan <i>Anacyclus clavatus</i>Mounia Chroho0Mustapha Aazza1Aziz Bouymajane2Yassine Oulad El Majdoub3Francesco Cacciola4Luigi Mondello5Touriya Zair6Latifa Bouissane7Molecular Chemistry, Materials and Catalysis Laboratory, Faculty of Sciences and Technologies, Sultan Moulay Slimane University, BP 523, Beni-Mellal 23000, MoroccoLaboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, BP 11201, Meknes 50070, MoroccoTeam of Microbiology and Health, Laboratory of Chemistry-Biology Applied to the Environment, Faculty of Sciences, Moulay Ismail University, BP 11201, Meknes 50070, MoroccoDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyResearch Team Chemistry of Bioactive Molecules and Environment, Laboratory of Innovative Materials and Biotechnologies of Natural Resources, Faculty of Sciences, Moulay Ismail University, BP 11201, Meknes 50070, MoroccoMolecular Chemistry, Materials and Catalysis Laboratory, Faculty of Sciences and Technologies, Sultan Moulay Slimane University, BP 523, Beni-Mellal 23000, MoroccoIn this work, polyphenols were extracted from <i>Anacylus clavatus</i> flowers using a hydroethanolic solvent, and the obtained extract was studied for its total phenol and flavonoid contents and evaluated for its antioxidant and antibacterial capacities. The contents of total phenols and flavonoids were measured by employing gallic acid and quercetin as references, respectively, and the phenolic composition analysis was conducted using high-performance liquid chromatography combined with a photodiode array and electrospray ionization mass spectrometry (HPLC-PDA/ESI-MS). The antioxidant capacity of the extracts was tested using a potassium ferric reducing antioxidant power (PFRAP) assay, and the antibacterial activity assay was carried out against Gram-negative bacteria (<i>Escherichia coli</i> and <i>Salmonella typhimirium</i>) and Gram-positive bacteria (<i>Staphyloccocus aureus</i> and <i>Listeria monocytogenes</i>) using the broth microdilution assay. The phenolic and flavonoid contents of the extracts equaled 9.53 ± 0.48 mg GAE/g dm and 1.31 ± 0.06 mg QE/g dm, respectively. The chromatographic analysis of the phenolic profile detected 26 phenolic compounds belonging to phenolic acids, flavones and flavonols, and with the caffeoylquinic acid derivatives being the major phenolic compounds present in 12 isomers. Only one organic compound, <i>viz.</i> citric acid, was found. The extracts exhibited interesting antioxidant activity. Bacteriostatic activity towards <i>Escherichia coli</i> and bactericidal activity against <i>Salmonella typhimirium</i>, <i>Staphyloccocus aureus</i> and <i>Listeria monocytogenes</i> were determined. This study revealed that <i>Anacyclus clavatus</i> flower extracts contain phenolic compounds with interesting bioactivities.https://www.mdpi.com/2223-7747/11/24/3423<i>Anacyclus clavatus</i>flower ethanolic extracttotal polyphenol contentflavonoid contentphenolic compositionFRAP activity |
| spellingShingle | Mounia Chroho Mustapha Aazza Aziz Bouymajane Yassine Oulad El Majdoub Francesco Cacciola Luigi Mondello Touriya Zair Latifa Bouissane HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan <i>Anacyclus clavatus</i> Plants <i>Anacyclus clavatus</i> flower ethanolic extract total polyphenol content flavonoid content phenolic composition FRAP activity |
| title | HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan <i>Anacyclus clavatus</i> |
| title_full | HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan <i>Anacyclus clavatus</i> |
| title_fullStr | HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan <i>Anacyclus clavatus</i> |
| title_full_unstemmed | HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan <i>Anacyclus clavatus</i> |
| title_short | HPLC-PDA/ESI-MS Analysis of Phenolic Compounds and Bioactivities of the Ethanolic Extract from Flowers of Moroccan <i>Anacyclus clavatus</i> |
| title_sort | hplc pda esi ms analysis of phenolic compounds and bioactivities of the ethanolic extract from flowers of moroccan i anacyclus clavatus i |
| topic | <i>Anacyclus clavatus</i> flower ethanolic extract total polyphenol content flavonoid content phenolic composition FRAP activity |
| url | https://www.mdpi.com/2223-7747/11/24/3423 |
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