Repair of full-thickness articular cartilage defects with a 3DP-anchored three-phase complex
Introduction: To repair cartilage defect as well as the calcified cartilage layer (CCL) and bone tissue, there is need to fabricate a three-phase complex that mimics the natural cartilage tissue. Materials and methods: SF/Col-Ⅱ/HA scaffolds were constructed by low-temperature 3D printing, and to pre...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023083317 |
| _version_ | 1827616114534776832 |
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| author | Kai Sun Ruixin Li Meng Fan |
| author_facet | Kai Sun Ruixin Li Meng Fan |
| author_sort | Kai Sun |
| collection | DOAJ |
| description | Introduction: To repair cartilage defect as well as the calcified cartilage layer (CCL) and bone tissue, there is need to fabricate a three-phase complex that mimics the natural cartilage tissue. Materials and methods: SF/Col-Ⅱ/HA scaffolds were constructed by low-temperature 3D printing, and to prepare a three-phase complex. The microstructure were showed using a SEM image analysis program. To observe collagen and glycosaminoglycan expression and analyze morphometric parameters, HE staining was performed to reveal new cartilage. Immunohistochemical were performed to investigate the collagen content and defect repair status in the new cartilage group in vitro and vivo. Results: Physical and biochemical properties and biocompatibility of three-phase complex met the requirements of constructing tissue-engineered cartilage. The OD values increased gradually at different time points. With increasing culture time, the OD values showed an upward trend. The HE and immunohistochemical staining results showed that new cartilage had formed at the defect and new cartilage formation occurred during in vivo repair. Conclusion: 3DP-anchored three-phase complexes have good physical and biochemical properties and biocompatibility and thus represent an alternative cartilage tissue engineering material. |
| first_indexed | 2024-03-09T09:21:32Z |
| format | Article |
| id | doaj.art-d1ced04515d84ae48690e8ec6a4d2a36 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:21:32Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-d1ced04515d84ae48690e8ec6a4d2a362023-12-02T07:01:16ZengElsevierHeliyon2405-84402023-11-01911e21123Repair of full-thickness articular cartilage defects with a 3DP-anchored three-phase complexKai Sun0Ruixin Li1Meng Fan2Tianjin First Central Hospital, Address:Baoshan West Road No. 2, Xiqing District, Tianjin, 300192, ChinaAcademy of Military and Medical Sciences, ChinaTianjin First Central Hospital, China; Corresponding authorIntroduction: To repair cartilage defect as well as the calcified cartilage layer (CCL) and bone tissue, there is need to fabricate a three-phase complex that mimics the natural cartilage tissue. Materials and methods: SF/Col-Ⅱ/HA scaffolds were constructed by low-temperature 3D printing, and to prepare a three-phase complex. The microstructure were showed using a SEM image analysis program. To observe collagen and glycosaminoglycan expression and analyze morphometric parameters, HE staining was performed to reveal new cartilage. Immunohistochemical were performed to investigate the collagen content and defect repair status in the new cartilage group in vitro and vivo. Results: Physical and biochemical properties and biocompatibility of three-phase complex met the requirements of constructing tissue-engineered cartilage. The OD values increased gradually at different time points. With increasing culture time, the OD values showed an upward trend. The HE and immunohistochemical staining results showed that new cartilage had formed at the defect and new cartilage formation occurred during in vivo repair. Conclusion: 3DP-anchored three-phase complexes have good physical and biochemical properties and biocompatibility and thus represent an alternative cartilage tissue engineering material.http://www.sciencedirect.com/science/article/pii/S2405844023083317Three-phase complexPrintingAnchoringScaffoldsBiocompatibility |
| spellingShingle | Kai Sun Ruixin Li Meng Fan Repair of full-thickness articular cartilage defects with a 3DP-anchored three-phase complex Heliyon Three-phase complex Printing Anchoring Scaffolds Biocompatibility |
| title | Repair of full-thickness articular cartilage defects with a 3DP-anchored three-phase complex |
| title_full | Repair of full-thickness articular cartilage defects with a 3DP-anchored three-phase complex |
| title_fullStr | Repair of full-thickness articular cartilage defects with a 3DP-anchored three-phase complex |
| title_full_unstemmed | Repair of full-thickness articular cartilage defects with a 3DP-anchored three-phase complex |
| title_short | Repair of full-thickness articular cartilage defects with a 3DP-anchored three-phase complex |
| title_sort | repair of full thickness articular cartilage defects with a 3dp anchored three phase complex |
| topic | Three-phase complex Printing Anchoring Scaffolds Biocompatibility |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023083317 |
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