Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome
Human immunodeficiency virus (HIV)-1 has a unique integration profile in the human genome relative to murine and avian retroviruses. Equine infectious anemia virus (EIAV) is another well-studied lentivirus that can also be used as a promising retro-transfection vector, but its integration into its n...
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MDPI AG
2015-06-01
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Online Access: | http://www.mdpi.com/1999-4915/7/6/2769 |
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author | Qiang Liu Xue-Feng Wang Jian Ma Xi-Jun He Xiao-Jun Wang Jian-Hua Zhou |
author_facet | Qiang Liu Xue-Feng Wang Jian Ma Xi-Jun He Xiao-Jun Wang Jian-Hua Zhou |
author_sort | Qiang Liu |
collection | DOAJ |
description | Human immunodeficiency virus (HIV)-1 has a unique integration profile in the human genome relative to murine and avian retroviruses. Equine infectious anemia virus (EIAV) is another well-studied lentivirus that can also be used as a promising retro-transfection vector, but its integration into its native host has not been characterized. In this study, we mapped 477 integration sites of the EIAV strain EIAVFDDV13 in fetal equine dermal (FED) cells during in vitro infection. Published integration sites of EIAV and HIV-1 in the human genome were also analyzed as references. Our results demonstrated that EIAVFDDV13 tended to integrate into genes and AT-rich regions, and it avoided integrating into transcription start sites (TSS), which is consistent with EIAV and HIV-1 integration in the human genome. Notably, the integration of EIAVFDDV13 favored long interspersed elements (LINEs) and DNA transposons in the horse genome, whereas the integration of HIV-1 favored short interspersed elements (SINEs) in the human genome. The chromosomal environment near LINEs or DNA transposons potentially influences viral transcription and may be related to the unique EIAV latency states in equids. The data on EIAV integration in its natural host will facilitate studies on lentiviral infection and lentivirus-based therapeutic vectors. |
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id | doaj.art-d1d6f746d02a49c6b804c18dfaf673ad |
institution | Directory Open Access Journal |
issn | 1999-4915 |
language | English |
last_indexed | 2024-12-13T21:53:39Z |
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spelling | doaj.art-d1d6f746d02a49c6b804c18dfaf673ad2022-12-21T23:30:12ZengMDPI AGViruses1999-49152015-06-01763241326010.3390/v7062769v7062769Characterization of Equine Infectious Anemia Virus Integration in the Horse GenomeQiang Liu0Xue-Feng Wang1Jian Ma2Xi-Jun He3Xiao-Jun Wang4Jian-Hua Zhou5State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaHuman immunodeficiency virus (HIV)-1 has a unique integration profile in the human genome relative to murine and avian retroviruses. Equine infectious anemia virus (EIAV) is another well-studied lentivirus that can also be used as a promising retro-transfection vector, but its integration into its native host has not been characterized. In this study, we mapped 477 integration sites of the EIAV strain EIAVFDDV13 in fetal equine dermal (FED) cells during in vitro infection. Published integration sites of EIAV and HIV-1 in the human genome were also analyzed as references. Our results demonstrated that EIAVFDDV13 tended to integrate into genes and AT-rich regions, and it avoided integrating into transcription start sites (TSS), which is consistent with EIAV and HIV-1 integration in the human genome. Notably, the integration of EIAVFDDV13 favored long interspersed elements (LINEs) and DNA transposons in the horse genome, whereas the integration of HIV-1 favored short interspersed elements (SINEs) in the human genome. The chromosomal environment near LINEs or DNA transposons potentially influences viral transcription and may be related to the unique EIAV latency states in equids. The data on EIAV integration in its natural host will facilitate studies on lentiviral infection and lentivirus-based therapeutic vectors.http://www.mdpi.com/1999-4915/7/6/2769equine infectious anemia virusintegration siteschromosomesRefSeq genesrepetitive elements |
spellingShingle | Qiang Liu Xue-Feng Wang Jian Ma Xi-Jun He Xiao-Jun Wang Jian-Hua Zhou Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome Viruses equine infectious anemia virus integration sites chromosomes RefSeq genes repetitive elements |
title | Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome |
title_full | Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome |
title_fullStr | Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome |
title_full_unstemmed | Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome |
title_short | Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome |
title_sort | characterization of equine infectious anemia virus integration in the horse genome |
topic | equine infectious anemia virus integration sites chromosomes RefSeq genes repetitive elements |
url | http://www.mdpi.com/1999-4915/7/6/2769 |
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