Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome

Human immunodeficiency virus (HIV)-1 has a unique integration profile in the human genome relative to murine and avian retroviruses. Equine infectious anemia virus (EIAV) is another well-studied lentivirus that can also be used as a promising retro-transfection vector, but its integration into its n...

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Main Authors: Qiang Liu, Xue-Feng Wang, Jian Ma, Xi-Jun He, Xiao-Jun Wang, Jian-Hua Zhou
Format: Article
Language:English
Published: MDPI AG 2015-06-01
Series:Viruses
Subjects:
Online Access:http://www.mdpi.com/1999-4915/7/6/2769
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author Qiang Liu
Xue-Feng Wang
Jian Ma
Xi-Jun He
Xiao-Jun Wang
Jian-Hua Zhou
author_facet Qiang Liu
Xue-Feng Wang
Jian Ma
Xi-Jun He
Xiao-Jun Wang
Jian-Hua Zhou
author_sort Qiang Liu
collection DOAJ
description Human immunodeficiency virus (HIV)-1 has a unique integration profile in the human genome relative to murine and avian retroviruses. Equine infectious anemia virus (EIAV) is another well-studied lentivirus that can also be used as a promising retro-transfection vector, but its integration into its native host has not been characterized. In this study, we mapped 477 integration sites of the EIAV strain EIAVFDDV13 in fetal equine dermal (FED) cells during in vitro infection. Published integration sites of EIAV and HIV-1 in the human genome were also analyzed as references. Our results demonstrated that EIAVFDDV13 tended to integrate into genes and AT-rich regions, and it avoided integrating into transcription start sites (TSS), which is consistent with EIAV and HIV-1 integration in the human genome. Notably, the integration of EIAVFDDV13 favored long interspersed elements (LINEs) and DNA transposons in the horse genome, whereas the integration of HIV-1 favored short interspersed elements (SINEs) in the human genome. The chromosomal environment near LINEs or DNA transposons potentially influences viral transcription and may be related to the unique EIAV latency states in equids. The data on EIAV integration in its natural host will facilitate studies on lentiviral infection and lentivirus-based therapeutic vectors.
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spelling doaj.art-d1d6f746d02a49c6b804c18dfaf673ad2022-12-21T23:30:12ZengMDPI AGViruses1999-49152015-06-01763241326010.3390/v7062769v7062769Characterization of Equine Infectious Anemia Virus Integration in the Horse GenomeQiang Liu0Xue-Feng Wang1Jian Ma2Xi-Jun He3Xiao-Jun Wang4Jian-Hua Zhou5State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, ChinaHuman immunodeficiency virus (HIV)-1 has a unique integration profile in the human genome relative to murine and avian retroviruses. Equine infectious anemia virus (EIAV) is another well-studied lentivirus that can also be used as a promising retro-transfection vector, but its integration into its native host has not been characterized. In this study, we mapped 477 integration sites of the EIAV strain EIAVFDDV13 in fetal equine dermal (FED) cells during in vitro infection. Published integration sites of EIAV and HIV-1 in the human genome were also analyzed as references. Our results demonstrated that EIAVFDDV13 tended to integrate into genes and AT-rich regions, and it avoided integrating into transcription start sites (TSS), which is consistent with EIAV and HIV-1 integration in the human genome. Notably, the integration of EIAVFDDV13 favored long interspersed elements (LINEs) and DNA transposons in the horse genome, whereas the integration of HIV-1 favored short interspersed elements (SINEs) in the human genome. The chromosomal environment near LINEs or DNA transposons potentially influences viral transcription and may be related to the unique EIAV latency states in equids. The data on EIAV integration in its natural host will facilitate studies on lentiviral infection and lentivirus-based therapeutic vectors.http://www.mdpi.com/1999-4915/7/6/2769equine infectious anemia virusintegration siteschromosomesRefSeq genesrepetitive elements
spellingShingle Qiang Liu
Xue-Feng Wang
Jian Ma
Xi-Jun He
Xiao-Jun Wang
Jian-Hua Zhou
Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome
Viruses
equine infectious anemia virus
integration sites
chromosomes
RefSeq genes
repetitive elements
title Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome
title_full Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome
title_fullStr Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome
title_full_unstemmed Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome
title_short Characterization of Equine Infectious Anemia Virus Integration in the Horse Genome
title_sort characterization of equine infectious anemia virus integration in the horse genome
topic equine infectious anemia virus
integration sites
chromosomes
RefSeq genes
repetitive elements
url http://www.mdpi.com/1999-4915/7/6/2769
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