Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones
A classical strategy to analyse the protein content of a biological sample is the two-dimensional gel electrophoresis (2D-GE). This technique separates proteins by both isoelectric point and molecular weight, and images are taken for subsequent analyses. However, analyses of 2D-GE images require st...
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| Format: | Article |
| Language: | Spanish |
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Instituto Tecnológico de Costa Rica
2022-11-01
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| Series: | Tecnología en Marcha |
| Subjects: | |
| Online Access: | https://172.20.14.50/index.php/tec_marcha/article/view/6452 |
| _version_ | 1797652971437162496 |
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| author | José Arturo Molina-Mora Diana Chinchilla-Montero Carolina Castro-Peña Fernando García |
| author_facet | José Arturo Molina-Mora Diana Chinchilla-Montero Carolina Castro-Peña Fernando García |
| author_sort | José Arturo Molina-Mora |
| collection | DOAJ |
| description |
A classical strategy to analyse the protein content of a biological sample is the two-dimensional gel electrophoresis (2D-GE). This technique separates proteins by both isoelectric point and molecular weight, and images are taken for subsequent analyses. However, analyses of 2D-GE images require standardized image analysis due to susceptibility of gels to get deformed, presence of overlapping spots and stripes, fuzzy and unstained spots, and others. This represent a difficulty for final users (researchers), which demand for free and user-friendly solutions. We have previously reported the standardization of a protocol to analyse 2D-GE images, and in the current study we applied it to two new bacterial isolates Pseudomonas aeruginosa C25 and C50. We first extracted periplasmic proteins after exposure to antibiotics, and we then run a 2D-GE analysis. Images were analysed using our standardized protocol, achieving the identification of protein spots using CellProfiler after pre-processing step. Comparison between strains was done using differential spot analysis, revealing a specific pattern in the protein expression between bacteria. These results will help to study the biological meaning of these strains using proteomic profiling under different conditions.
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| first_indexed | 2024-03-11T16:37:30Z |
| format | Article |
| id | doaj.art-d1e073068657438e934ffbfebb5a94f8 |
| institution | Directory Open Access Journal |
| issn | 0379-3982 2215-3241 |
| language | Spanish |
| last_indexed | 2024-03-11T16:37:30Z |
| publishDate | 2022-11-01 |
| publisher | Instituto Tecnológico de Costa Rica |
| record_format | Article |
| series | Tecnología en Marcha |
| spelling | doaj.art-d1e073068657438e934ffbfebb5a94f82023-10-23T14:27:36ZspaInstituto Tecnológico de Costa RicaTecnología en Marcha0379-39822215-32412022-11-0135810.18845/tm.v35i8.6452Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clonesJosé Arturo Molina-MoraDiana Chinchilla-MonteroCarolina Castro-PeñaFernando García A classical strategy to analyse the protein content of a biological sample is the two-dimensional gel electrophoresis (2D-GE). This technique separates proteins by both isoelectric point and molecular weight, and images are taken for subsequent analyses. However, analyses of 2D-GE images require standardized image analysis due to susceptibility of gels to get deformed, presence of overlapping spots and stripes, fuzzy and unstained spots, and others. This represent a difficulty for final users (researchers), which demand for free and user-friendly solutions. We have previously reported the standardization of a protocol to analyse 2D-GE images, and in the current study we applied it to two new bacterial isolates Pseudomonas aeruginosa C25 and C50. We first extracted periplasmic proteins after exposure to antibiotics, and we then run a 2D-GE analysis. Images were analysed using our standardized protocol, achieving the identification of protein spots using CellProfiler after pre-processing step. Comparison between strains was done using differential spot analysis, revealing a specific pattern in the protein expression between bacteria. These results will help to study the biological meaning of these strains using proteomic profiling under different conditions. https://172.20.14.50/index.php/tec_marcha/article/view/64522D-GEImage analysisCellProfilerP. aeruginosa C25P. aeruginosa C50 |
| spellingShingle | José Arturo Molina-Mora Diana Chinchilla-Montero Carolina Castro-Peña Fernando García Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones Tecnología en Marcha 2D-GE Image analysis CellProfiler P. aeruginosa C25 P. aeruginosa C50 |
| title | Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones |
| title_full | Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones |
| title_fullStr | Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones |
| title_full_unstemmed | Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones |
| title_short | Two-dimensional gel electrophoresis image analysis of two Pseudomonas aeruginosa clones |
| title_sort | two dimensional gel electrophoresis image analysis of two pseudomonas aeruginosa clones |
| topic | 2D-GE Image analysis CellProfiler P. aeruginosa C25 P. aeruginosa C50 |
| url | https://172.20.14.50/index.php/tec_marcha/article/view/6452 |
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