Comparative Proteomics Study on the Postharvest Senescence of <i>Volvariella volvacea</i>
<i>Volvariella volvacea</i> is difficult to store after harvest, which restricts the production and circulation of <i>V. volvacea</i> fruiting bodies. Low-temperature storage is the traditional storage method used for most edible fungi. However, <i>V. volvacea</i>...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/8/819 |
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| author | Lei Zha Mingjie Chen Qian Guo Zongjun Tong Zhengpeng Li Changxia Yu Huanling Yang Yan Zhao |
| author_facet | Lei Zha Mingjie Chen Qian Guo Zongjun Tong Zhengpeng Li Changxia Yu Huanling Yang Yan Zhao |
| author_sort | Lei Zha |
| collection | DOAJ |
| description | <i>Volvariella volvacea</i> is difficult to store after harvest, which restricts the production and circulation of <i>V. volvacea</i> fruiting bodies. Low-temperature storage is the traditional storage method used for most edible fungi. However, <i>V. volvacea</i> undergoes autolysis at low temperatures. When fruiting bodies are stored at 15 °C (suitable temperature), <i>V. volvacea</i> achieves the best fresh-keeping effect. However, the molecular mechanism underlying the postharvest senescence of <i>V. volvacea</i> remains unclear. Based on this information, we stored <i>V. volvacea</i> fruiting bodies at 15 °C after harvest and then analyzed the texture and phenotype combined with the results of previous physiological research. Four time points (0, 24, 60, and 96 h) were selected for the comparative proteomics study of <i>V. volvacea</i> during storage at 15 °C. A variety of proteins showed differential expressions in postharvest <i>V. volvacea</i> at 15 °C. Further comparison of the gene ontology (GO) enrichment analysis and KEGG pathways performed at different sampling points revealed proteins that were significantly enriched at several time points. At the same time, we also analyzed differentially expressed proteins (DEPs) related to the RNA transport, fatty acid biosynthesis and metabolism, and amino acid biosynthesis and metabolism pathways, and discussed the molecular functions of the PAB1, RPG1, ACC1, ADH3, ADH2, ALD5, and SDH2 proteins in postharvest <i>V. volvacea</i> senescence. Our results showed that many biological processes of the postharvest senescence of <i>V. volvacea</i> changed. Most importantly, we found that most RNA transport-related proteins were down-regulated, which may lead to a decrease in related gene regulation. Our results also showed that the expression of other important proteins, such as the fatty acid metabolism related proteins increased; and changes in fatty acid composition affected the cell membrane, which may accelerate the ripening and perception of <i>V. volvacea</i> fruiting bodies. Therefore, our research provides a reference for further studies on the aging mechanism of <i>V. volvacea</i>. |
| first_indexed | 2024-03-09T04:15:26Z |
| format | Article |
| id | doaj.art-d1e1a27823374ec9b15ca3b3fe2b602b |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T04:15:26Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-d1e1a27823374ec9b15ca3b3fe2b602b2023-12-03T13:55:11ZengMDPI AGJournal of Fungi2309-608X2022-08-018881910.3390/jof8080819Comparative Proteomics Study on the Postharvest Senescence of <i>Volvariella volvacea</i>Lei Zha0Mingjie Chen1Qian Guo2Zongjun Tong3Zhengpeng Li4Changxia Yu5Huanling Yang6Yan Zhao7Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, ChinaInstitute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China<i>Volvariella volvacea</i> is difficult to store after harvest, which restricts the production and circulation of <i>V. volvacea</i> fruiting bodies. Low-temperature storage is the traditional storage method used for most edible fungi. However, <i>V. volvacea</i> undergoes autolysis at low temperatures. When fruiting bodies are stored at 15 °C (suitable temperature), <i>V. volvacea</i> achieves the best fresh-keeping effect. However, the molecular mechanism underlying the postharvest senescence of <i>V. volvacea</i> remains unclear. Based on this information, we stored <i>V. volvacea</i> fruiting bodies at 15 °C after harvest and then analyzed the texture and phenotype combined with the results of previous physiological research. Four time points (0, 24, 60, and 96 h) were selected for the comparative proteomics study of <i>V. volvacea</i> during storage at 15 °C. A variety of proteins showed differential expressions in postharvest <i>V. volvacea</i> at 15 °C. Further comparison of the gene ontology (GO) enrichment analysis and KEGG pathways performed at different sampling points revealed proteins that were significantly enriched at several time points. At the same time, we also analyzed differentially expressed proteins (DEPs) related to the RNA transport, fatty acid biosynthesis and metabolism, and amino acid biosynthesis and metabolism pathways, and discussed the molecular functions of the PAB1, RPG1, ACC1, ADH3, ADH2, ALD5, and SDH2 proteins in postharvest <i>V. volvacea</i> senescence. Our results showed that many biological processes of the postharvest senescence of <i>V. volvacea</i> changed. Most importantly, we found that most RNA transport-related proteins were down-regulated, which may lead to a decrease in related gene regulation. Our results also showed that the expression of other important proteins, such as the fatty acid metabolism related proteins increased; and changes in fatty acid composition affected the cell membrane, which may accelerate the ripening and perception of <i>V. volvacea</i> fruiting bodies. Therefore, our research provides a reference for further studies on the aging mechanism of <i>V. volvacea</i>.https://www.mdpi.com/2309-608X/8/8/819<i>V. volvacea</i>postharvestsenescencecomparative proteomics |
| spellingShingle | Lei Zha Mingjie Chen Qian Guo Zongjun Tong Zhengpeng Li Changxia Yu Huanling Yang Yan Zhao Comparative Proteomics Study on the Postharvest Senescence of <i>Volvariella volvacea</i> Journal of Fungi <i>V. volvacea</i> postharvest senescence comparative proteomics |
| title | Comparative Proteomics Study on the Postharvest Senescence of <i>Volvariella volvacea</i> |
| title_full | Comparative Proteomics Study on the Postharvest Senescence of <i>Volvariella volvacea</i> |
| title_fullStr | Comparative Proteomics Study on the Postharvest Senescence of <i>Volvariella volvacea</i> |
| title_full_unstemmed | Comparative Proteomics Study on the Postharvest Senescence of <i>Volvariella volvacea</i> |
| title_short | Comparative Proteomics Study on the Postharvest Senescence of <i>Volvariella volvacea</i> |
| title_sort | comparative proteomics study on the postharvest senescence of i volvariella volvacea i |
| topic | <i>V. volvacea</i> postharvest senescence comparative proteomics |
| url | https://www.mdpi.com/2309-608X/8/8/819 |
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