Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains
Staphylococcus epidermidis is a leading cause of nosocomial infections, majorly resistant to beta-lactam antibiotics, and may transfer several mobile genetic elements among the members of its own species, as well as to Staphylococcus aureus; however, a genetic exchange from S. aureus to S. epidermid...
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MDPI AG
2018-02-01
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| Series: | Toxins |
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| Online Access: | http://www.mdpi.com/2072-6651/10/3/93 |
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| author | Xavier Argemi Chimène Nanoukon Dissou Affolabi Daniel Keller Yves Hansmann Philippe Riegel Lamine Baba-Moussa Gilles Prévost |
| author_facet | Xavier Argemi Chimène Nanoukon Dissou Affolabi Daniel Keller Yves Hansmann Philippe Riegel Lamine Baba-Moussa Gilles Prévost |
| author_sort | Xavier Argemi |
| collection | DOAJ |
| description | Staphylococcus epidermidis is a leading cause of nosocomial infections, majorly resistant to beta-lactam antibiotics, and may transfer several mobile genetic elements among the members of its own species, as well as to Staphylococcus aureus; however, a genetic exchange from S. aureus to S. epidermidis remains controversial. We recently identified two pathogenic clinical strains of S. epidermidis that produce a staphylococcal enterotoxin C3-like (SEC) similar to that by S. aureus pathogenicity islands. This study aimed to determine the genetic environment of the SEC-coding sequence and to identify the mobile genetic elements. Whole-genome sequencing and annotation of the S. epidermidis strains were performed using Illumina technology and a bioinformatics pipeline for assembly, which provided evidence that the SEC-coding sequences were located in a composite pathogenicity island that was previously described in the S. epidermidis strain FRI909, called SePI-1/SeCI-1, with 83.8–89.7% nucleotide similarity. Various other plasmids were identified, particularly p_3_95 and p_4_95, which carry antibiotic resistance genes (hsrA and dfrG, respectively), and share homologies with SAP085A and pUSA04-2-SUR11, two plasmids described in S. aureus. Eventually, one complete prophage was identified, ΦSE90, sharing 30 out of 52 coding sequences with the Acinetobacter phage vB_AbaM_IME200. Thus, the SePI-1/SeCI-1 pathogenicity island was identified in two pathogenic strains of S. epidermidis that produced a SEC enterotoxin causing septic shock. These findings suggest the existence of in vivo genetic exchange from S. aureus to S. epidermidis. |
| first_indexed | 2024-04-11T20:48:41Z |
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| language | English |
| last_indexed | 2024-04-11T20:48:41Z |
| publishDate | 2018-02-01 |
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| series | Toxins |
| spelling | doaj.art-d1e49f12576d43b69d227221c128c5792022-12-22T04:03:57ZengMDPI AGToxins2072-66512018-02-011039310.3390/toxins10030093toxins10030093Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic StrainsXavier Argemi0Chimène Nanoukon1Dissou Affolabi2Daniel Keller3Yves Hansmann4Philippe Riegel5Lamine Baba-Moussa6Gilles Prévost7Service des Maladies Infectieuses et Tropicales, Hôpitaux Universitaires, Strasbourg 67000, FranceFaculté des Sciences et Techniques, Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey, Calavi, Cotonou 05 BP 1604, BeninFaculté des Sciences et Techniques, Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey, Calavi, Cotonou 05 BP 1604, BeninEA 7290, Virulence Bactérienne précoce, Université de Strasbourg, CHRU Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, Strasbourg 67000, FranceService des Maladies Infectieuses et Tropicales, Hôpitaux Universitaires, Strasbourg 67000, FranceEA 7290, Virulence Bactérienne précoce, Université de Strasbourg, CHRU Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, Strasbourg 67000, FranceFaculté des Sciences et Techniques, Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Université d’Abomey, Calavi, Cotonou 05 BP 1604, BeninEA 7290, Virulence Bactérienne précoce, Université de Strasbourg, CHRU Strasbourg, Fédération de Médecine Translationnelle de Strasbourg, Strasbourg 67000, FranceStaphylococcus epidermidis is a leading cause of nosocomial infections, majorly resistant to beta-lactam antibiotics, and may transfer several mobile genetic elements among the members of its own species, as well as to Staphylococcus aureus; however, a genetic exchange from S. aureus to S. epidermidis remains controversial. We recently identified two pathogenic clinical strains of S. epidermidis that produce a staphylococcal enterotoxin C3-like (SEC) similar to that by S. aureus pathogenicity islands. This study aimed to determine the genetic environment of the SEC-coding sequence and to identify the mobile genetic elements. Whole-genome sequencing and annotation of the S. epidermidis strains were performed using Illumina technology and a bioinformatics pipeline for assembly, which provided evidence that the SEC-coding sequences were located in a composite pathogenicity island that was previously described in the S. epidermidis strain FRI909, called SePI-1/SeCI-1, with 83.8–89.7% nucleotide similarity. Various other plasmids were identified, particularly p_3_95 and p_4_95, which carry antibiotic resistance genes (hsrA and dfrG, respectively), and share homologies with SAP085A and pUSA04-2-SUR11, two plasmids described in S. aureus. Eventually, one complete prophage was identified, ΦSE90, sharing 30 out of 52 coding sequences with the Acinetobacter phage vB_AbaM_IME200. Thus, the SePI-1/SeCI-1 pathogenicity island was identified in two pathogenic strains of S. epidermidis that produced a SEC enterotoxin causing septic shock. These findings suggest the existence of in vivo genetic exchange from S. aureus to S. epidermidis.http://www.mdpi.com/2072-6651/10/3/93pathogenicity islandenterotoxinStaphylococcus epidermidisStaphylococcus aureuspan-genomecore genome |
| spellingShingle | Xavier Argemi Chimène Nanoukon Dissou Affolabi Daniel Keller Yves Hansmann Philippe Riegel Lamine Baba-Moussa Gilles Prévost Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains Toxins pathogenicity island enterotoxin Staphylococcus epidermidis Staphylococcus aureus pan-genome core genome |
| title | Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains |
| title_full | Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains |
| title_fullStr | Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains |
| title_full_unstemmed | Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains |
| title_short | Comparative Genomics and Identification of an Enterotoxin-Bearing Pathogenicity Island, SEPI-1/SECI-1, in Staphylococcus epidermidis Pathogenic Strains |
| title_sort | comparative genomics and identification of an enterotoxin bearing pathogenicity island sepi 1 seci 1 in staphylococcus epidermidis pathogenic strains |
| topic | pathogenicity island enterotoxin Staphylococcus epidermidis Staphylococcus aureus pan-genome core genome |
| url | http://www.mdpi.com/2072-6651/10/3/93 |
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