Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance
The resorption rate of autologous fat transfer (AFT) is 40–60% of the implanted tissue, requiring new surgical strategies for tissue reconstruction. We previously demonstrated in a rabbit model that AFT may be empowered by adipose-derived mesenchymal stromal/stem cells (AD-MSCs), which improve graft...
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MDPI AG
2022-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/18/2799 |
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| author | Maria Serena Piccinno Tiziana Petrachi Marco Pignatti Alba Murgia Giulia Grisendi Olivia Candini Elisa Resca Valentina Bergamini Francesco Ganzerli Alberto Portone Ilenia Mastrolia Chiara Chiavelli Ilaria Castelli Daniela Bernabei Mara Tagliazucchi Elisa Bonetti Francesca Lolli Giorgio De Santis Massimo Dominici Elena Veronesi |
| author_facet | Maria Serena Piccinno Tiziana Petrachi Marco Pignatti Alba Murgia Giulia Grisendi Olivia Candini Elisa Resca Valentina Bergamini Francesco Ganzerli Alberto Portone Ilenia Mastrolia Chiara Chiavelli Ilaria Castelli Daniela Bernabei Mara Tagliazucchi Elisa Bonetti Francesca Lolli Giorgio De Santis Massimo Dominici Elena Veronesi |
| author_sort | Maria Serena Piccinno |
| collection | DOAJ |
| description | The resorption rate of autologous fat transfer (AFT) is 40–60% of the implanted tissue, requiring new surgical strategies for tissue reconstruction. We previously demonstrated in a rabbit model that AFT may be empowered by adipose-derived mesenchymal stromal/stem cells (AD-MSCs), which improve graft persistence by exerting proangiogenic/anti-inflammatory effects. However, their fate after implantation requires more investigation. We report a xenograft model of adipose tissue engineering in which NOD/SCID mice underwent AFT with/without human autologous AD-MSCs and were monitored for 180 days (d). The effect of AD-MSCs on AFT grafting was also monitored by evaluating the expression of CD31 and F4/80 markers. Green fluorescent protein-positive AD-MSCs (AD-MSC-GFP) were detected in fibroblastoid cells 7 days after transplantation and in mature adipocytes at 60 days, indicating both persistence and differentiation of the implanted cells. This evidence also correlated with the persistence of a higher graft weight in AFT-AD-MSC compared to AFT alone treated mice. An observation up to 180 d revealed a lower resorption rate and reduced lipidic cyst formation in the AFT-AD-MSC group, suggesting a long-term action of AD-MSCs in support of AFT performance and an anti-inflammatory/proangiogenic activity. Together, these data indicate the protective role of adipose progenitors in autologous AFT tissue resorption. |
| first_indexed | 2024-03-10T00:27:03Z |
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| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:27:03Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-d1e7e99019fd4c70b0a55be18001f7b92023-11-23T15:32:24ZengMDPI AGCells2073-44092022-09-011118279910.3390/cells11182799Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation PerformanceMaria Serena Piccinno0Tiziana Petrachi1Marco Pignatti2Alba Murgia3Giulia Grisendi4Olivia Candini5Elisa Resca6Valentina Bergamini7Francesco Ganzerli8Alberto Portone9Ilenia Mastrolia10Chiara Chiavelli11Ilaria Castelli12Daniela Bernabei13Mara Tagliazucchi14Elisa Bonetti15Francesca Lolli16Giorgio De Santis17Massimo Dominici18Elena Veronesi19Technopole “Mario Veronesi”, Via 29 Maggio 6, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio 6, 41037 Mirandola, ItalyPlastic Surgery, IRCCS Azienda Ospedaliero-Universitaria Sant’Orsola di Bologna, Via Massarenti 9, 40138 Bologna, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio 6, 41037 Mirandola, ItalyDepartment of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Hospital of Modena, Via del Pozzo, 71, 41125 Modena, ItalyRigenerand Srl, Via Maestri del Lavoro 4, 41036 Medolla, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio 6, 41037 Mirandola, ItalyClinical and Experimental Medicine PhD Program, University of Modena and Reggio Emilia, 41125 Modena, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio 6, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio 6, 41037 Mirandola, ItalyDepartment of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Hospital of Modena, Via del Pozzo, 71, 41125 Modena, ItalyDepartment of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Hospital of Modena, Via del Pozzo, 71, 41125 Modena, ItalyDepartment of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Hospital of Modena, Via del Pozzo, 71, 41125 Modena, ItalyDepartment of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Hospital of Modena, Via del Pozzo, 71, 41125 Modena, ItalyHematological Diagnostics and Clinical Genomics Unit, Modena University Hospital, Via del Pozzo 71, 41125 Modena, ItalyDepartment of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Hospital of Modena, Via del Pozzo, 71, 41125 Modena, ItalyDepartment of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Hospital of Modena, Via del Pozzo, 71, 41125 Modena, ItalyDepartment of Medical and Surgical Sciences for Children & Adults, University of Modena and Reggio Emilia, Hospital of Modena, Via del Pozzo, 71, 41125 Modena, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio 6, 41037 Mirandola, ItalyTechnopole “Mario Veronesi”, Via 29 Maggio 6, 41037 Mirandola, ItalyThe resorption rate of autologous fat transfer (AFT) is 40–60% of the implanted tissue, requiring new surgical strategies for tissue reconstruction. We previously demonstrated in a rabbit model that AFT may be empowered by adipose-derived mesenchymal stromal/stem cells (AD-MSCs), which improve graft persistence by exerting proangiogenic/anti-inflammatory effects. However, their fate after implantation requires more investigation. We report a xenograft model of adipose tissue engineering in which NOD/SCID mice underwent AFT with/without human autologous AD-MSCs and were monitored for 180 days (d). The effect of AD-MSCs on AFT grafting was also monitored by evaluating the expression of CD31 and F4/80 markers. Green fluorescent protein-positive AD-MSCs (AD-MSC-GFP) were detected in fibroblastoid cells 7 days after transplantation and in mature adipocytes at 60 days, indicating both persistence and differentiation of the implanted cells. This evidence also correlated with the persistence of a higher graft weight in AFT-AD-MSC compared to AFT alone treated mice. An observation up to 180 d revealed a lower resorption rate and reduced lipidic cyst formation in the AFT-AD-MSC group, suggesting a long-term action of AD-MSCs in support of AFT performance and an anti-inflammatory/proangiogenic activity. Together, these data indicate the protective role of adipose progenitors in autologous AFT tissue resorption.https://www.mdpi.com/2073-4409/11/18/2799adipogenesisadipose stem cellsmesenchymal stem cells (MSCs)tissue engineering |
| spellingShingle | Maria Serena Piccinno Tiziana Petrachi Marco Pignatti Alba Murgia Giulia Grisendi Olivia Candini Elisa Resca Valentina Bergamini Francesco Ganzerli Alberto Portone Ilenia Mastrolia Chiara Chiavelli Ilaria Castelli Daniela Bernabei Mara Tagliazucchi Elisa Bonetti Francesca Lolli Giorgio De Santis Massimo Dominici Elena Veronesi Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance Cells adipogenesis adipose stem cells mesenchymal stem cells (MSCs) tissue engineering |
| title | Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance |
| title_full | Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance |
| title_fullStr | Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance |
| title_full_unstemmed | Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance |
| title_short | Human Adipose Mesenchymal Stromal/Stem Cells Improve Fat Transplantation Performance |
| title_sort | human adipose mesenchymal stromal stem cells improve fat transplantation performance |
| topic | adipogenesis adipose stem cells mesenchymal stem cells (MSCs) tissue engineering |
| url | https://www.mdpi.com/2073-4409/11/18/2799 |
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