Antimicrobial, modulatory and chemical analysis of the oil of Croton limae
Context: Croton sp. are plants with a well-reported antimicrobial activity. Croton limae A.P. Gomes, M.F. Sales P.E. Berry (Euphorbiaceae), known as ‘marmeleiro-prateado’, is commonly used to manage abdominal pain in Brazil. Objective: This work evaluates the phytochemical composition, antimicrobial...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-01-01
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| Series: | Pharmaceutical Biology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/13880209.2017.1355926 |
| _version_ | 1818249038797996032 |
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| author | Tiago Rodrigues Leite Maria Arlene Pessoa da Silva Antônio Carlito Bezerra dos Santos Henrique Douglas Melo Coutinho Antonia Eliene Duarte José Galberto Martins da Costa |
| author_facet | Tiago Rodrigues Leite Maria Arlene Pessoa da Silva Antônio Carlito Bezerra dos Santos Henrique Douglas Melo Coutinho Antonia Eliene Duarte José Galberto Martins da Costa |
| author_sort | Tiago Rodrigues Leite |
| collection | DOAJ |
| description | Context: Croton sp. are plants with a well-reported antimicrobial activity. Croton limae A.P. Gomes, M.F. Sales P.E. Berry (Euphorbiaceae), known as ‘marmeleiro-prateado’, is commonly used to manage abdominal pain in Brazil. Objective: This work evaluates the phytochemical composition, antimicrobial and modulatory activities of the essential oil of C. limae leaves (EOCL). Materials and methods: The minimum inhibitory concentration (MIC) and the modulation of the antibiotic activity were determined using a microdilution method. The concentration of EOCL ranged between 512 and 8 μg/mL. Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Candida tropicalis, C. krusei and C. albicans strains were used in the MIC and modulation assays. The antibiotics, amikacin, gentamicin and neomycin, and the antifungals, amphotericin B, benzoylmetronidazole and nystatin, were used in concentrations ranging between 2500 and 2.5 μg/mL. The phytochemical analysis of the EOCL was performed through gas chromatography coupled to a mass spectrometer (GC/MS). Results: Only Staphylococcus aureus was inhibited by a clinically relevant concentration of EOCL (MIC 512 μg/mL). Synergism between the EOCL and amikacin against S. aureus (9.76 μg/mL) and E. coli (39.062 μg/mL); neomycin against E. coli (2.44 μg/mL); and benzoylmetronidazole against C. krusei (256 μg/mL) were observed. The GC/MS analysis identified cedrol, eucalyptol and α-pinene as the main compounds of EOCL. Conclusion: EOCL inhibited the growth of S. aureus and potentiated the antibiotic and antifungal effects of drugs against all bacterial and Candida strains, respectively. |
| first_indexed | 2024-12-12T15:30:06Z |
| format | Article |
| id | doaj.art-d1e83fcca8b54d47bfca5d280211cc05 |
| institution | Directory Open Access Journal |
| issn | 1388-0209 1744-5116 |
| language | English |
| last_indexed | 2024-12-12T15:30:06Z |
| publishDate | 2017-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Pharmaceutical Biology |
| spelling | doaj.art-d1e83fcca8b54d47bfca5d280211cc052022-12-22T00:20:08ZengTaylor & Francis GroupPharmaceutical Biology1388-02091744-51162017-01-015512015201910.1080/13880209.2017.13559261355926Antimicrobial, modulatory and chemical analysis of the oil of Croton limaeTiago Rodrigues Leite0Maria Arlene Pessoa da Silva1Antônio Carlito Bezerra dos Santos2Henrique Douglas Melo Coutinho3Antonia Eliene Duarte4José Galberto Martins da Costa5Cariri Regional UniversityCariri Regional UniversityRegional University of CaririCariri Regional UniversityRegional University of CaririCariri Regional UniversityContext: Croton sp. are plants with a well-reported antimicrobial activity. Croton limae A.P. Gomes, M.F. Sales P.E. Berry (Euphorbiaceae), known as ‘marmeleiro-prateado’, is commonly used to manage abdominal pain in Brazil. Objective: This work evaluates the phytochemical composition, antimicrobial and modulatory activities of the essential oil of C. limae leaves (EOCL). Materials and methods: The minimum inhibitory concentration (MIC) and the modulation of the antibiotic activity were determined using a microdilution method. The concentration of EOCL ranged between 512 and 8 μg/mL. Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumonia, Candida tropicalis, C. krusei and C. albicans strains were used in the MIC and modulation assays. The antibiotics, amikacin, gentamicin and neomycin, and the antifungals, amphotericin B, benzoylmetronidazole and nystatin, were used in concentrations ranging between 2500 and 2.5 μg/mL. The phytochemical analysis of the EOCL was performed through gas chromatography coupled to a mass spectrometer (GC/MS). Results: Only Staphylococcus aureus was inhibited by a clinically relevant concentration of EOCL (MIC 512 μg/mL). Synergism between the EOCL and amikacin against S. aureus (9.76 μg/mL) and E. coli (39.062 μg/mL); neomycin against E. coli (2.44 μg/mL); and benzoylmetronidazole against C. krusei (256 μg/mL) were observed. The GC/MS analysis identified cedrol, eucalyptol and α-pinene as the main compounds of EOCL. Conclusion: EOCL inhibited the growth of S. aureus and potentiated the antibiotic and antifungal effects of drugs against all bacterial and Candida strains, respectively.http://dx.doi.org/10.1080/13880209.2017.1355926marmeleiro-prateadostaphylococcus aureusescherichia coli; pseudomonas aeruginosaklebsiella pneumoniacandida tropicaliscandida kruseicandida albicansessential leaf oilchemical constituents |
| spellingShingle | Tiago Rodrigues Leite Maria Arlene Pessoa da Silva Antônio Carlito Bezerra dos Santos Henrique Douglas Melo Coutinho Antonia Eliene Duarte José Galberto Martins da Costa Antimicrobial, modulatory and chemical analysis of the oil of Croton limae Pharmaceutical Biology marmeleiro-prateado staphylococcus aureus escherichia coli; pseudomonas aeruginosa klebsiella pneumonia candida tropicalis candida krusei candida albicans essential leaf oil chemical constituents |
| title | Antimicrobial, modulatory and chemical analysis of the oil of Croton limae |
| title_full | Antimicrobial, modulatory and chemical analysis of the oil of Croton limae |
| title_fullStr | Antimicrobial, modulatory and chemical analysis of the oil of Croton limae |
| title_full_unstemmed | Antimicrobial, modulatory and chemical analysis of the oil of Croton limae |
| title_short | Antimicrobial, modulatory and chemical analysis of the oil of Croton limae |
| title_sort | antimicrobial modulatory and chemical analysis of the oil of croton limae |
| topic | marmeleiro-prateado staphylococcus aureus escherichia coli; pseudomonas aeruginosa klebsiella pneumonia candida tropicalis candida krusei candida albicans essential leaf oil chemical constituents |
| url | http://dx.doi.org/10.1080/13880209.2017.1355926 |
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