A Poly(dA:dT) Tract in the <i>IGF1</i> Gene Is a Genetic Marker for Growth Traits in Pigs
Insulin-like growth factor 1 (<i>IGF1</i>) is an important regulator of body growth, development, and metabolism. The poly(dA:dT) tract affects the accessibility of transcription factor binding sites to regulate transcription. Therefore, this study assessed the effects of two poly(dA:dT)...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/2076-2615/12/23/3316 |
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| author | Weili Liao Yifei Wang Xiwu Qiao Xiaoke Zhang Haohui Deng Caihong Zhang Jiaqi Li Xiaolong Yuan Hao Zhang |
| author_facet | Weili Liao Yifei Wang Xiwu Qiao Xiaoke Zhang Haohui Deng Caihong Zhang Jiaqi Li Xiaolong Yuan Hao Zhang |
| author_sort | Weili Liao |
| collection | DOAJ |
| description | Insulin-like growth factor 1 (<i>IGF1</i>) is an important regulator of body growth, development, and metabolism. The poly(dA:dT) tract affects the accessibility of transcription factor binding sites to regulate transcription. Therefore, this study assessed the effects of two poly(dA:dT) tracts on the transcriptional activity of porcine <i>IGF1</i>. The luciferase assay results demonstrated that the poly(dA:dT) tract 2 (−264/−255) was a positive regulatory element for <i>IGF1</i> gene expression, and the activities between the different lengths of the poly(dA:dT) tract 2 were significant (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>p</mi><mo><</mo><mn>0.01</mn></mrow></semantics></math></inline-formula>). The transcription factor <i>C/EBPα</i> inhibited the transcription of <i>IGF1</i> by binding to tract 2, and the expression levels between the lengths of tract 2 after <i>C/EBPα</i> binding were also statistically different (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>p</mi><mo><</mo><mn>0.01</mn></mrow></semantics></math></inline-formula>). Only the alleles 10T and 11T were found in the tract 2 in commercial pig breeds, while the 9T, 10T, and 11T alleles were found in Chinese native pig breeds. The allele frequencies were in Hardy–Weinberg equilibrium in all pig breeds. The genotypes of tract 2 were significantly associated with the growth traits (days to 115 kg and average daily gain) (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>p</mi><mo><</mo><mn>0.05</mn></mrow></semantics></math></inline-formula>) in commercial pig breeds. Based on these findings, it can be concluded that the tract 2 mutation could be applied as a candidate genetic marker for growth trait selection in pig breeding programs. |
| first_indexed | 2024-03-09T17:55:38Z |
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| institution | Directory Open Access Journal |
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| publishDate | 2022-11-01 |
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| spelling | doaj.art-d1e89ec785ff42a396e661cdc5e74b0b2023-11-24T10:25:06ZengMDPI AGAnimals2076-26152022-11-011223331610.3390/ani12233316A Poly(dA:dT) Tract in the <i>IGF1</i> Gene Is a Genetic Marker for Growth Traits in PigsWeili Liao0Yifei Wang1Xiwu Qiao2Xiaoke Zhang3Haohui Deng4Caihong Zhang5Jiaqi Li6Xiaolong Yuan7Hao Zhang8Guangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangzhou Xustoms Technology Center, Guangzhou 510623, ChinaGuangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Laboratory of Lingnan Modern Agriculture, National Engineering Research Center for Breeding Swine Industry, Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaInsulin-like growth factor 1 (<i>IGF1</i>) is an important regulator of body growth, development, and metabolism. The poly(dA:dT) tract affects the accessibility of transcription factor binding sites to regulate transcription. Therefore, this study assessed the effects of two poly(dA:dT) tracts on the transcriptional activity of porcine <i>IGF1</i>. The luciferase assay results demonstrated that the poly(dA:dT) tract 2 (−264/−255) was a positive regulatory element for <i>IGF1</i> gene expression, and the activities between the different lengths of the poly(dA:dT) tract 2 were significant (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>p</mi><mo><</mo><mn>0.01</mn></mrow></semantics></math></inline-formula>). The transcription factor <i>C/EBPα</i> inhibited the transcription of <i>IGF1</i> by binding to tract 2, and the expression levels between the lengths of tract 2 after <i>C/EBPα</i> binding were also statistically different (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>p</mi><mo><</mo><mn>0.01</mn></mrow></semantics></math></inline-formula>). Only the alleles 10T and 11T were found in the tract 2 in commercial pig breeds, while the 9T, 10T, and 11T alleles were found in Chinese native pig breeds. The allele frequencies were in Hardy–Weinberg equilibrium in all pig breeds. The genotypes of tract 2 were significantly associated with the growth traits (days to 115 kg and average daily gain) (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>p</mi><mo><</mo><mn>0.05</mn></mrow></semantics></math></inline-formula>) in commercial pig breeds. Based on these findings, it can be concluded that the tract 2 mutation could be applied as a candidate genetic marker for growth trait selection in pig breeding programs.https://www.mdpi.com/2076-2615/12/23/3316<i>IGF1</i>luciferase assaytranscription regulationpoly(dA:dT) tracts |
| spellingShingle | Weili Liao Yifei Wang Xiwu Qiao Xiaoke Zhang Haohui Deng Caihong Zhang Jiaqi Li Xiaolong Yuan Hao Zhang A Poly(dA:dT) Tract in the <i>IGF1</i> Gene Is a Genetic Marker for Growth Traits in Pigs Animals <i>IGF1</i> luciferase assay transcription regulation poly(dA:dT) tracts |
| title | A Poly(dA:dT) Tract in the <i>IGF1</i> Gene Is a Genetic Marker for Growth Traits in Pigs |
| title_full | A Poly(dA:dT) Tract in the <i>IGF1</i> Gene Is a Genetic Marker for Growth Traits in Pigs |
| title_fullStr | A Poly(dA:dT) Tract in the <i>IGF1</i> Gene Is a Genetic Marker for Growth Traits in Pigs |
| title_full_unstemmed | A Poly(dA:dT) Tract in the <i>IGF1</i> Gene Is a Genetic Marker for Growth Traits in Pigs |
| title_short | A Poly(dA:dT) Tract in the <i>IGF1</i> Gene Is a Genetic Marker for Growth Traits in Pigs |
| title_sort | poly da dt tract in the i igf1 i gene is a genetic marker for growth traits in pigs |
| topic | <i>IGF1</i> luciferase assay transcription regulation poly(dA:dT) tracts |
| url | https://www.mdpi.com/2076-2615/12/23/3316 |
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